EGFR mutations detection on liquid-based cytology: Is microscopy still necessary?

Journal of Clinical Pathology

Volumn 65, Issue 6, 2012, Pages 561-564

  Malapelle, Umberto ^a   De Rosa, Nicla ^b   Bellevicine, Claudio ^a   Rocco, Danilo ^b   Vitiello, Fabiana ^b   Piantedosi, Franco Vito ^b   Illiano, Alfonso ^b   Nappi, Oscar ^c   Troncone, Giancarlo ^a,d  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b AORN Vincenzo Monaldi   (Italy)

^c CARDARELLI HOSPITAL   (Italy)

^d CEINGE BIOTECNOLOGIE AVANZATE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; VASCULOTROPIN RECEPTOR;

ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; CYTOLOGY; DNA EXTRACTION; GENE MUTATION; GENE SEQUENCE; HUMAN; HUMAN CELL; LASER CAPTURE MICRODISSECTION; LIQUID BASED CYTOLOGY; PAPANICOLAOU TEST; PRIORITY JOURNAL; STANDARDIZATION;

ADENOCARCINOMA; CARCINOMA, NON-SMALL-CELL LUNG; CARCINOMA, SQUAMOUS CELL; CYTODIAGNOSIS; CYTOLOGICAL TECHNIQUES; DIAGNOSIS, DIFFERENTIAL; DNA MUTATIONAL ANALYSIS; DNA, NEOPLASM; HUMANS; LASER CAPTURE MICRODISSECTION; LUNG NEOPLASMS; MICROSCOPY; MUTATION; RECEPTOR, EPIDERMAL GROWTH FACTOR;

EID: 84861808093     PISSN: 00219746     EISSN: 14724146     Source Type: Journal    
DOI: 10.1136/jclinpath-2011-200659     Document Type: Article

References (9)

1. Malapelle U, Bellevicine C, Zeppa P, et al. Cytology-based gene mutation tests to predict response to anti-epidermal growth factor receptor therapy: a review. Diagn Cytopathol 2011;39:703-10.
2. Clark DP. Seize the opportunity: underutilization of fine-needle aspiration biopsy to inform targeted cancer therapy decisions. Cancer 2009;117:289-97.
3. Malapelle U, de Rosa N, Rocco D, et al. EGFR and KRAS mutations detection on lung cancer liquid-based cytology: a pilot study. J Clin Pathol 2012;65:87-91.
4. Savic S, Tapia C, Grilli B, et al. Comprehensive epidermal growth factor receptor gene analysis from cytological specimens of non-small-cell lung cancers. Br J Cancer 2008;98:154-60.
5. Smith GD, Chadwick BE, Willmore-Payne C, et al. Detection of epidermal growth factor receptor gene mutations in cytology specimens from patients with non-small cell lung cancer utilising high-resolution melting amplicon analysis. J Clin Pathol 2008;61:487-93. (Pubitemid 351518232)
6. Rosell R, Moran T, Queralt C, et al. Screening for epidermal growth factor receptor mutations in lung cancer. N Engl J Med 2009;361:958-67.
7. Nicholson AG, Gonzalez D, Shah P, et al. Refining the diagnosis and EGFR status of non-small cell lung carcinoma in biopsy and cytologic material, using a panel of mucin staining, TTF-1, cytokeratin 5/6, and P63, and EGFR mutation analysis. J Thorac Oncol 2010;5:436-41.
8. Tanaka T, Nagai Y, Miyazawa H, et al. Reliability of the peptide nucleic acid-locked nucleic acid polymerase chain reaction clamp-based test for epidermal growth factor receptor mutations integrated into the clinical practice for non-small cell lung cancers. Cancer Sci 2007;98:246-52. (Pubitemid 44941878)
9. Fassina A, Gazziero A, Zardo D, et al. Detection of EGFR and KRAS mutations on trans-thoracic needle aspiration of lung nodules by high resolution melting analysis. J Clin Pathol 2009;62:1096-102.