Using bleach-chase to measure protein half-lives in living cells

Nature Protocols

Volumn 7, Issue 4, 2012, Pages 801-811

  Geva Zatorsky, Naama ^a   Issaeva, Irina ^a   Mayo, Avi ^a   Cohen, Ariel ^a   Dekel, Erez ^a   Danon, Tamar ^a   Cohen, Lydia ^a   Liron, Yuvalal ^a   Alon, Uri ^a   Eden, Eran ^a,b  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b MeMed Diagnostics   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BLEACHING; CELL ASSAY; CELL CLONE; CELL GROWTH; CELL LINE; CHROMOSOMAL LOCALIZATION; CLINICAL PROTOCOL; DILUTION; FLUORESCENCE MICROSCOPY; HALF LIFE TIME; IMAGE ANALYSIS; INTRACELLULAR SIGNALING; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN METABOLISM;

BACTERIAL PROTEINS; BLEACHING AGENTS; GENES, REPORTER; HALF-LIFE; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PROTEINS; PROTEOLYSIS; TIME-LAPSE IMAGING;

EID: 84861782481     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2012.028     Document Type: Article

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