Molecular identification and antibiotic control of endophytic bacterial contaminants from micropropagated Aglaonema cultures

Plant Cell, Tissue and Organ Culture

Volumn 110, Issue 1, 2012, Pages 53-62

  Fang, Jong Yi ^a   Hsu, Yu Rong ^a  

^a NATIONAL PINGTUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Taiwan)

Author keywords

Aglaonema; Antibiotic control; Endophytic bacteria; In vitro contaminant; Molecular identification

Indexed keywords

16S RIBOSOMAL RNA; AGLAONEMA; ANTIBIOTIC TREATMENT; ANTIMICROBIAL SUSCEPTIBILITY; BACTERIAL CONTAMINANTS; BACTERIAL CONTAMINATION; BACTERIAL GROWTH; BACTERIAL SPECIES; CULTURE MEDIUM; ENDOPHYTIC BACTERIA; GROWTH SUBSTRATES; IN-VITRO; IRRIGATION WATERS; LOW CONCENTRATIONS; MICROPROPAGATION; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR IDENTIFICATION; NODAL EXPLANTS; PARTIAL SEQUENCES; SOIL AND WATER; STOCK PLANTS;

ANTIBIOTICS; CONTAMINATION; ECOLOGY; GENES; IDENTIFICATION (CONTROL SYSTEMS); IMPURITIES; IRRIGATION; PLANTS (BOTANY); RNA; WATER SUPPLY;

BACTERIA;

AGLAONEMA; BACTERIA (MICROORGANISMS);

EID: 84861758480     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-012-0129-6     Document Type: Article

References (53)

1. Attree SM, Sheffield E (1986) An evaluation of ficoll density gradient centrifugation as a method for eliminating microbial contamination and purifying plant protoplasts. Plant Cell Rep 5: 288-291.
2. Bacon CW, Glenn AE, Hinton DM (2002) Isolation, in planta detection and culture of endophytic bacteria and fungi. In: Hurst CJ, Crawford RL, McInerney MJ, Knudsen GR, Stetzenbach LD (eds) Manual of environmental microbiology, 2nd edn. ASM Press, Washington, pp 542-553.
3. Bauer RW, Kirby MDK, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by standard single disc diffusion method. Am J Clin Pathol 45: 493-496.
4. Bosshard PP, Abels S, Zbinden R, Bottger EC, Altwegg M (2003) Ribosomal DNA sequencing for identification of aerobic gram-positive rods in the clinical laboratory (an 18-Month Evaluation). J Clin Microbiol 41: 4134-4140.
5. Brunner I, Echegaray A, Rubluo A (1995) Isolation and characterization of bacterial contaminants from Dieffenbachia amoena Bull, Anthurium andreanum Linden and Spathiphyllum sp. shoot cultured in vitro. Sci Hortic 62: 103-111.
6. Buckley PM, DeWilde TN, Reed BM (1995) Characterization and identification of bacteria isolated from micropropagated mint plants. In Vitro Cell Dev Biol-Plant 31P: 58-64.
7. Cerritos R, Vinuesa P, Eguiarte LE, Herrera-Estrella L, Alcaraz-Peraza LD, Arvizu-Gómez JL, Olmedo G, Ramirez E, Siefert JL, Souza V (2008) Bacillus coahuilensis sp. nov., a moderately halophilic species from a desiccaion lagoon in the Cuatro Ciénegas Valley in Coahuila, Mexico. Int J Syst Evol Microbiol 58: 919-923.
8. Chen WL, Yeh DM (2007) Elimination of in vitro contamination, shoot multiplication, and ex vitro rooting of Aglaonema. HortScience 42: 629-632.
9. Chen J, Henny RJ, McConnell DB (2002) Development of new foliage plant cultivars. In: Janick J, Whipkey A (eds) Trends in new crops and new uses. Timber Press, Portland, pp 466-472.
10. Clinical and Laboratory Standards Institute (2007) Disk diffusion supplemental tables. Document M100-S17. CLSI, Wayne.
11. Dias SCF, Costa FEC, Andreote FD, Lacava PT, Teixeira MA, Assumpçao LC, Araújo WL, Azevedo JL, Melo IS (2009) Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion. World J Microbiol Biotechnol 25: 189-195.
12. Duncan DB (1955) Multiple range and multiple F-tests. Biometrics 11: 1-42.
13. Falkiner FR (1988) Strategy for the selection of antibiotics for use against common bacterial pathogens and endophytes of plants. Acta Hortic 225: 53-56.
14. Fisse L, Batalle A, Pera J (1987) Endogenous bacteria elimination in ornamental plants. Acta Hortic 212: 87-90.
15. Grisham MP, Pan Y-B, Richard EP Jr (2007) Early detection of Leifsonia xyli subsp. xyli in sugarcane leaves by real-time polymerase chain reaction. Plant Dis 91: 430-434.
16. Gunson HE, Spencer-Phillips PTN (1994) Latent bacterial infections: epiphytes and endophytes as contaminants of micropropagated plants. In: Nicholas JR (ed) Physiology, growth and development of plants in culture. Kluwer Academic Publishers, Dordrecht, pp 379-396.
17. Hallmann J, Quadt-Hallmann A, Mahaffe WF, Kloepper JW (1997) Bacterial endophytes in agricultural crops. Can J Microbiol 43: 895-914.
18. Herman EB (2004) Recent advances in plant tissue culture VIII. Microbial contaminants in plant tissue cultures: solutions and opportunities 1996-2003. Agritech Consultants Inc, Shrub Oak.
19. Hirano SS, Upper CD (1990) Population biology and epidemiology of Pseudomonas syringae. Annu Rev Phytopathol 28: 155-177.
20. Horsch RB, King J (1983) A covert contaminant of cultured plant cells: elimination of a Hyphomicrobium spp. from cultures of Datura innoxia (Mill.). Plant Cell Tissue Organ Cult 2: 21-28.
21. Hung PQ, Kumar SM, Govindsamy V, Annapurna K (2007) Isolation and characterization of endophytic bacteria from wild and cultivated soybean varieties. Biol Fertil Soils 44: 155-162.
22. Kneifel W, Leonhardt W (1992) Testing of different antibiotics against gram positive and gram negative bacteria isolated from plant tissue cultures. Plant Cell Tissue Organ Cult 29: 139-144.
23. Kritzinger EM, Vuuren RJV, Woodward B, Rong IH, Spreeth MH, Slabbert MM (1998) Elimination of external and internal contaminants in rhizomes of Zantedeschia aethiopica with commercial fungicides and antibiotics. Plant Cell Tissue Organ Cult 52: 61-65.
24. Kunisaki JT (1980) In vitro propagation of Anthurium andreanum Lind. HortScience 15: 508-509.
25. Lata H, Li XC, Silva B, Moraes RM, Halda-Alija L (2006) Identification of IAA-producing endophytic bacteria from micropropagated Echinaceae plants using 16S rRNA sequencing. Plant Cell Tissue Organ Cult 85: 353-359.
26. Leifert C, Cassells AC (2001) Microbial hazards in plant tissue and cell cultures. In Vitro Cell Develop Biol Plant 37: 133-138.
27. Leifert C, Waites WM (1992) Bacterial growth in plant tissue culture media. J Appl Bacteriol 72: 460-466.
28. Leifert C, Ritchie JY, Waites WM (1991) Contaminants of plant tissue and cell cultures. World J Microbiol Biotechnol 7: 452-469.
29. Leifert C, Camotta H, Waites WM (1992) Effect of combinations of antibiotics on micropropagated Clematis, Delphinium, Hosta, Iris and Photinia. Plant Cell Tissue Organ Cult 29: 153-160.
30. Leifert C, Morris CE, Waites WM (1994) Ecology of microbial saprophytes and pathogens in tissue culture and field-grown plants: reasons for contamination problems in vitro. Crit Rev Plant Sci 13: 139-183.
31. Liu T-H, Hsu N-W, Wu R-Y (2005) Control of leaf-tip necrosis of micropropagated ornamental statice by elimination of endophytic bacteria. In Vitro Cell Develop Biol Plant 41: 546-549.
32. Lodewyckx C, Vangronsveld J, Porteous Moore F, Moore ERB, Taghavi S, van der Lelie D (2002) Endophytic bacteria and their potential applications. Crit Rev Plant Sci 21: 583-606.
33. Misra P, Gupta N, Toppo DD, Pandey V, Mishra MK, Tuli R (2010) Establishment of long-term proliferating shoot cultures of elite Jatropha curcas L. by controlling endophytic bacterial contamination. Plant Cell Tissue Organ Cult 100: 189-197.
34. ™). Plant Cell Tissue Organ Cult 102: 365-372.
35. Mizra MS, Ahmad W, Latif F, Haurat J, Bally R, Normand P, Malik KA (2001) Isolation, partial characterization, and the effect of plant growth-promoting bacteria (PGPB) on micropropagated sugarcane in vitro. Plant Soil 237: 47-54.
36. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.
37. NCCLS (1997) Performance Standards for Antimicrobial disk susceptibility tests: approved standard M2-A7. National Committee for Clinical Laboratory Standards, Wayne.
38. Nowak J, Asiedu SK, Bensalim S, Richards J, Stewart A, Smith C, Stevens D, Sturz AV (1998) From laboratory to applications: challenges and progress with in vitro dual cultures of potato and beneficial bacteria. Plant Cell Tissue Organ Cult 52: 97-103.
39. Nuebel U, Engelen B, Felske A, Snaidr J, Weishuber A, Amann RI (1996) Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J Bacteriol 178: 5636-5643.
40. Reed BM, Buckley PM, DeWilde TN (1995) Detection and eradication of endophytic bacteria from micropropagated mint plants. In Vitro Cell Develop Biol Plant 31: 53-57.
41. SAS Institute Inc. (1999) Version 8. 02. SAS Institute, Cary.
42. Seabrook JM, Farrell G (1993) City water can contaminate tissue culture stock plants. HortScience 28: 628-629.
43. Shehata AM, Wannarat W, Skirvin RM, Norton MA (2010) The dual role of carbenicillin in shoot regeneration and somatic embryogenesis of horseradish (Armoracia rusticana) in vitro. Plant Cell Tissue Organ Cult 102: 397-402.
44. Shen H, Li Z, Han D, Yang F, Huang Q, Ran L (2010) Detection of indigenous endophytic bacteria in Eucalyptus urophylla in vitro conditions. Front Agric China 4: 37-41.
45. Sun L, Qiu F, Zhang X, Dai X, Dong X, Song W (2008) Endophytic bacterial diversity in rice (Oryza sativa L.) roots estimated by 16S rDNA sequence analysis. Microbiol Ecol 55: 415-424.
46. Thomas P (2004) In vitro decline in plant cultures: detection of a legion of covert bacteria as the cause for degenetation of long-term micropropagated troploid watermelon cultures. Plant Cell Tissue Organ Cult 77: 173-179.
47. Thomas P, Prakash GS (2004) Sanitizing long-term micropropagated grapes from covert and endophytic bacteria and preliminary field testing of plants after 8 years in vitro. In Vitro Cell Develop Biol Plant 40: 603-607.
48. Thomas P, Kumari S, Swarna GK, Prakash DP, Dinesh MR (2007) Ubiquitous presence of fastidious endophytic bacteria in field shoots and index-negative apparently clean shoot-tip cultures of papaya. Plant Cell Rep 26: 1491-1499.
49. Thomas P, Swarna GK, Patil P, Rawal RD (2008) Ubiquitous presence of normally non-culturable endophytic bacteria in field shoot-tips of banana and their gradual activation to quiescent cultivable form in tissue cultures. Plant Cell Tissue Organ Cult 93: 39-54.
50. Thomas P, Goplakrishnan C, Krishnareddy M (2011) Soft rot inciting Pectobacterium carotovorum (syn. Erwinia carotovora) is unlikely to be transmitted as a latent pathogen in micropropagated banana. Plant Cell Tissue Organ Cult 105: 423-429.
51. Wackerow-Kouzova ND (2007) Ochrobactrum intermedium ANKI, a nitrogen-fixing bacterium able to decolorize azobenzene. Appl Biochem Microbiol 43: 403-406.
52. Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173: 697-703.
53. Yu Z, Morrison M (2004) Comparison of different hypervariabile regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturating gradient gel electrophoresis. Appl Environ Microbiol 70: 4800-4806.