Marker-free transgenic durum wheat cv. Karim expressing the AlSAP gene exhibits a high level of tolerance to salinity and dehydration stresses

Molecular Breeding

Volumn 30, Issue 1, 2012, Pages 521-533

  Ben Saad, Rania ^a   Ben Ramdhan, Walid ^a   Zouari, Nabil ^a   Azaza, Jalel ^a   Mieulet, Delphine ^b   Guiderdoni, Emmanuel ^b,c   Ellouz, Radhouane ^a   Hassairi, Afif ^a,c  

^a UNIVERSITY OF SFAX   (Tunisia)

^b UMR AGAP   (France)

^c KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

Abiotic stresses; Aeluropus littoralis; AlSAP; Durum wheat; In embryo transformation; Marker free transgenic plants

Indexed keywords

GENE EXPRESSION; GRAIN (AGRICULTURAL PRODUCT); PLANTS (BOTANY);

ABIOTIC STRESS; AELUROPUS LITTORALI; ALSAP; DROUGHT STRESS; DURUM WHEATS; IN-EMBRYO TRANSFORMATION; MARKER-FREE; MARKER-FREE TRANSGENIC PLANT; TRANSGENEIC PLANTS; TRANSGENICS;

DROUGHT;

AELUROPUS LITTORALIS; NICOTIANA TABACUM; TRITICUM AESTIVUM; TRITICUM TURGIDUM SUBSP. DURUM;

EID: 84861725507     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11032-011-9641-3     Document Type: Article

References (22)

1. Ayers RS, Westcot DW (1985) Water quality for agriculture. FAO Irrigation Drainage Paper 29: 1-174.
2. Ben Saad R, Zouari N, Ben Ramdhan W, Azaza J, Meynard D, Guiderdoni E, Hassairi A (2010) Improved drought and salt stress tolerance in transgenic tobacco overexpressing a novel A20/AN1 zinc-finger "AlSAP" gene isolated from the halophyte grass Aeluropus littoralis. Plant Mol Biol 72: 171-190.
3. Chen H, Nelson RS, Sherwood JL (1994) Enhanced recovery of transformants of Agrobacterium tumifaciens after freeze-thaw transformation and drug selection. Biotechniques 16: 664-668.
4. FAO (2009) Crop prospects and food situation. No. 2 April 2009.
5. Garfinkel DJ, Nester EW (1980) Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. J Bacteriol 144: 732-743.
6. Hassairi A, Ben Saad R, Zouari N, Ben Ramdhan W, Azaza J (2008) A method for in embryo transformation with Agrobacterium of wheat and production of transgenic fertile plants over-expressing the AlSAP gene, isolated from the halophyte grass A. littoralis, Tunisian. Patent SN08356 17-09-2008.
7. Hood EE, Gelvin SB, Melchers S, Hoekema A (1993) New Agrobacterium helper plasmids for gene transfer to plant. Transgenic Res 2: 208-218.
8. Kanneganti V, Gupta AK (2008) Overexpression of OsiSAP8, a member of stress associated protein (SAP) gene family of rice confers tolerance to salt, drought and cold stress in transgenic tobacco and rice. Plant Mol Biol 66: 445-462.
9. Katerji N, van Hoorn JW, Hamdy A, Mastrorilli M, Nachit MM, Oweis T (2005) Salt tolerance analysis of chickpea, faba bean and durum wheat varieties. Agric Water Manage 72: 195-207.
10. Katerji N, Mastrorilli M, van Hoornc JW, Lahmerd FZ, Hamdyd A, Oweise T (2009) Durum wheat and barley productivity in saline-drought environments. Eur J Agron 3: 1-9.
11. Katiyar-Agarwal S, Kapoor A, Grover A (2002) Binary cloning vectors for efficient genetic transformation of rice. Curr Sci 7: 873-876.
12. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8: 4321-4325.
13. Ohira K, Ojima K, Fujiwara A (1973) Studies on the nutrition of rice cell culture 1: a simple defined medium for rapid growth in suspension culture. Plant Cell Physiol 14: 1113-1121.
14. Rezgui M, Ben Mechlia N, Bizid E, Kalboussi R, Hayouni R (2000) Étude de la stabilité du rendement de blé dur dans différentes régions de la Tunisie. In: L'amélioration du blé dur dans la région méditerranéenne: nouveaux défis. Options méditerranéennes Sér. A: Séminaires Méditerranéennes 40: 167-172.
15. Sakiss N, Ennabli N, Slimani MS, Baccour H (1994) La pluviométrie en Tunisie a-t-elle changé depuis 2000 ans? Recherche de tendance et de cycles dans les séries pluviométriques. Institut National de la Météorologie, 283 p.
16. Savé R, Alegre L, Pery M, Terradas J (1993) Ecophysiology of after-fire resprouts of Arbutus unedo L. Orsis 8: 107-119.
17. Sreenivasulu N, Varshney R, Kavi Kishor P, Weschke W (2004) Functional genomics for tolerance to abiotic stress in cereals. In: Gupta, Pushpendra Kumar, Varshney RK (eds) Cereal genomics. Kluwer Academic Publishers, Dordrecht, pp 483-514.
18. Supartana P, Shimizu T, Nogawa M, Shioiri H, Nakajima T, Haramoto N, Nozue M, Kojima M (2006) Development of simple and efficient in-planta transformation method for wheat (Triticum aestivum L.) using Agrobacterium tumefaciens. J Biosci Bioeng 102: 162-170.
19. Turner NC (1981) Techniques and experimental approaches for the measurement of plant water status. Plant Soil 58: 339-366.
20. Vij S, Tyagi AK (2007) Emerging trends in the functional genomics of the abiotic stressresponse in crop plants. Plant Biotechnol J 5: 361-380.
21. Wang H, Qi M, Cutler AJ (1993) A simple method of preparing plant samples for PCR. Nucleic Acid Res 21: 4153-4154.
22. Zouari N, Ben Saad R, Legavre Th, Azaza J, Sabau X, Jaoua M, Masmoudi K, Hassairi A (2007) Identification and sequencing of ESTs from the halophyte grass Aeluropus littoralis. Gene 404: 61-69.