The use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins

Electrophoresis

Volumn 33, Issue 9-10, 2012, Pages 1349-1355

  Ma, Xiao ^a   Wildgruber, Robert ^b   Bauer, Johann ^c   Weber, Gerhard ^b   Infanger, Manfred ^d   Grosse, Jirka ^e   Grimm, Daniela ^a,d  

^a AARHUS UNIVERSITY   (Denmark)

^b FFE Service GmbH   (Germany)

^c MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^d OTTO VON GUERICKE UNIVERSITY   (Germany)

^e UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

Cytoskeletal proteins; Glycolytic enzymes; Isoelectric point; Phosphoglycerate kinase; Polypeptide chain cleavage

Indexed keywords

ELECTROPHORESIS; ENZYMES; QUALITY CONTROL;

CYTOSKELETAL PROTEINS; FREE-FLOW ELECTROPHORESIS; GLYCOLYTIC ENZYME; ISO-ELECTRIC POINTS; PH GRADIENTS; PHOSPHOGLYCERATE KINASE; POLYPEPTIDE CHAIN CLEAVAGE; POLYPEPTIDES CHAINS; PREFRACTIONATION; SIGMOIDS;

ENDOTHELIAL CELLS;

ACTIN; CELL PROTEIN; CYTOPLASM PROTEIN; CYTOPLASMIC ACTIN; ENDOTHELIAL CELL PROTEIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MYOSIN; TRIOSEPHOSPHATE ISOMERASE; UNCLASSIFIED DRUG; VIMENTIN;

ARTICLE; BIOTECHNOLOGY; ENDOTHELIUM CELL; FREE FLOW ISOELECTRIC FOCUSING; HUMAN; HUMAN CELL; ISOELECTRIC FOCUSING; PH; PROTEIN ANALYSIS; PROTEIN ISOLATION; SEPARATION TECHNIQUE; SIGMOID PH GRADIENT;

EID: 84861683765     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.201100598     Document Type: Article

References (35)

1. Ly, L., Wasinger, V. C., Proteomics 2011, 11, 513-534.
2. Celis, J. E., Olsen, E., Electrophoresis 1994, 15, 309-344.
3. Zola, H., Neoh, S. H., Mantzioris, B. X., Webster, J., Loughnan, M. S., J. Immunol. Meth. 1990, 135, 247-255.
4. Malmstroem, J., Lee, H., Nesvizhskii, A. I., Shteynberg, D., Mohanty, S., Brunner, E., Ye, M., Weber, G., Eckerskorn, C., Aebersold, R., J. Proteome Res. 2006, 5, 2241-2249.
5. Rabilloud, T., Proteomics 2002, 2, 3-10.
6. Thorsell, A., Portelius, E., Blennow, K., Westman-Brinkmalm, A., Rapid Commun. Mass Spectrometry 2007, 21, 771-778.
7. Lim, P., North, R., Vigh, G., Electrophoresis 2007, 28, 1851-1859.
8. Moritz, R. L., Skandarajah, A. R., Ji, H., Simpson, R. J., Comp. Funct. Genom. 2005, 6, 236-243.
9. Nissum, M., Foucher, A. L., Expert Rev. Proteomics 2008, 5, 571-587.
10. Choi, C. W., Hong, Y. S., Kim, S. I., J. Microbiol. 2008, 46, 448-455.
11. Obermaier, C., Jankowski, V., Schmutzler, C., Bauer, J., Wildgruber, R., Infanger, M., Köhrle, J., Krause, E., Weber, G., Grimm, D., Electrophoresis 2005, 26, 2109-2116.
12. Weber, P., Weber, G., Posch, A., Eckerskorn, C., Free-Flow IEF, Lab Manuals, Cold Spring Harbor Laboratory Press, Plainview, New York 2003.
13. Pietsch, J., Kussian, R., Sickmann, A., Bauer, J., Weber, G., Nissum, M., Westphal, K., Egli, M., Grosse, J., Schoenberger, J., Wildgruber, R., Infanger, M., Grimm, D., Proteomics 2010, 10, 904-913.
14. Pietsch, J., Sickmann, A., Weber G., Bauer, J., Egli, M., Wildgruber, R., Infanger, M., Grimm, D. Proteomics 2011, 11, 2095-2104.
15. Edgell, C. J., Mc Donald, C. C., Graham, J. B., Proc. Natl. Acad. Sci U S A 1983, 80, 3734-3737.
16. Infanger, M., Kossmehl, P., Shakibaei, M., Baatout, S., Witzing, A., Grosse, J., Bauer, J., Cogoli, A., Faramarzi, S., Derradji, H., Neefs, M., Paul, M., Grimm, D., Apoptosis 2006, 11, 749-764.
17. Grosse, J., Wehland, M., Pietsch, J., Ma, X., Ulbrich, C., Schulz, H., Saar, K., Hübner, N., Hauslage, J., Hemmersbach, R., Braun, M., van Loon, J., Vagt, N., Infanger, M., Eilles, C., Egli, M., Richter, P., Baltz, T., Einspanier, R., Sharbati, S., Grimm, D., FASEB J 2012, 26, 639-655.
18. Grimm, D., Bauer, J., Kossmehl, P., Shakibaei, M., Schonberger, J., Pickenhahn, H., Schulze-Tanzil, G., Vetter, R., Eilles, C., Paul, M., Cogoli, A., FASEB J. 2002, 16, 604-616.
19. Grimm, D., Infanger, M., Westphal, K., Ulbrich, C., Pietsch, J., Kossmehl, P., Vadrucci, S., Baatout, S., Flick, B., Paul, M., Bauer, J., Tissue Eng. Part A 2009, 15, 2267-2275.
20. Grimm, D., Bauer, J., Ulbrich, C., Westphal, K., Wehland, M., Infanger, M., Aleshcheva, G., Pietsch, J., Ghardi, M., Beck, M., El-Saghire, H., de Saint-Georges, L., Baatout, S., Tissue Eng. Part A 2010, 16, 1559-1573.
21. Infanger, M., Ulbrich, C., Baatout, S., Wehland, M., Kreutz, R., Bauer, J., Grosse, J., Vadrucci, S., Cogoli, A., Derradji, H., Neefs, M., Küsters, S., Spain, M., Paul, M., Grimm, D., J. Cell. Biochem. 2007, 101, 1439-1455.
22. Ulbrich, C., Westphal, K., Baatout, S., Wehland, M., Bauer, J., Flick, B., Infanger, M., Kreutz, R., Vadrucci, S., Egli, M., Cogoli, A., Derradji, H., Pietsch, J., Paul, M., Grimm, D., J Cell. Biochem. 2008, 104, 1324-1341.
23. White, R. J., Averner, M., Nature 2001, 409, 1115-1118.
24. Pietsch, J., Bauer, J., Egli, M., Infanger, M., Wise, P., Ulbrich, C., Grimm, D., Curr. Mol. Med. 2011, 11, 350-364.
25. Caspers, M. L., Posey, Y., Brown, R. K., Anal. Biochem. 1977, 79, 166-180.
26. Brown, R. K., Caspers M. L., Lull, J. M., Vinogradov, S. N., Felgenhauer, K., Nekic, M., J. Chromatograph. 1977, 131, 223-232.
27. Righetti, P. G., Isoelectric Focusing: Theory, Methodology and Applications, Elsevier, Amsterdam 1983, pp. 283-287.
28. Righetti, P. G., Immobilized pH Gradients: Theory and Methodology, Elsevier, Amsterdam 1990.
29. Kossmehl, P., Kurth, E., Faramarzi, S., Habighorst, B., Shakibaei, M., Wehland, M., Kreutz, R., Infanger, M., Danser, A. H., Grosse, J., Paul, M., Grimm, D., Apoptosis 2006, 11, 347-358.
30. Rothermund, L., Kreutz, R., Kossmehl, P., Fredersdorf, S., Shakibaei, M., Schulze-Tanzil, G., Paul, M., Grimm, D., Am. J. Hypertens. 2002, 15, 644-652.
31. Riecke, K., Grimm, D., Shakibaei, M., Kossmehl, P., Schulze-Tanzil, G., Paul, M., Stahlmann, R., Arch. Toxicol. 2002, 76, 360-366.
32. Lavastre, V., Binet, F., Moisan, E., Chiasson, S., Girard, D., Brit. J. Haematol. 2007, 138, 545-554.
33. Carlile, G. W., Chalmers-Redman, R. M.E., Tatton, N. A., Pong, A., Borden, K. E., Tatton, G. T., Mol. Pharmacol. 2000, 57, 2-12.
34. Grigorieva, J. A., Dainiak, M. B., Katrukha, A. G., Muronetz, V. I., Archiv. Biochem. Biophys. 1999, 369, 252-260.
35. Fokina, K. V., Dainyak, M. B., Nagradova, N. K., Muronetz, V. I., Archiv. Biochem. Biophys. 1997, 345, 185-192.