Generation of transgenic zebrafish with liver-specific expression of EGFP-Lc3: A new in vivo model for investigation of liver autophagy

Biochemical and Biophysical Research Communications

Volumn 422, Issue 2, 2012, Pages 268-273

  Cui, Jianzhou ^a   Sim, Tina Huey Fen ^a   Gong, Zhiyuan ^a   Shen, Han Ming ^a,b  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Autophagy; Lc3; Liver; MTOR; Torin1; Zebrafish

Indexed keywords

CHLOROQUINE; ENHANCED GREEN FLUORESCENT PROTEIN; LC3 PROTEIN; PROTEIN; TORIN 1; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; AUTOPHAGOSOME; AUTOPHAGY; CONTROLLED STUDY; EMBRYO DEVELOPMENT; HUMAN; IN VIVO STUDY; LARVA; LIVER AUTOPHAGY; LIVER CELL; LYSOSOME; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; UPREGULATION; ZEBRA FISH;

AMINO ACID SEQUENCE; ANIMALS; ANIMALS, GENETICALLY MODIFIED; AUTOPHAGY; CHLOROQUINE; GREEN FLUORESCENT PROTEINS; LIVER; MICE; MICROTUBULE-ASSOCIATED PROTEINS; MODELS, ANIMAL; MOLECULAR SEQUENCE DATA; NAPHTHYRIDINES; UP-REGULATION; ZEBRAFISH;

ANIMALIA; DANIO RERIO; MUS;

EID: 84861658299     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.04.145     Document Type: Article

References (37)

1. Klionsky D.J., Emr S.D. Autophagy as a regulated pathway of cellular degradation. Science 2000, 290:1717-1721.
2. Maiuri M.C., Zalckvar E., Kimchi A., Kroemer G. Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat. Rev. Mol. Cell Biol. 2007, 8:741-752.
3. Xie Z., Klionsky D.J. Autophagosome formation: core machinery and adaptations. Nat. Cell Biol. 2007, 9:1102-1109.
4. Yang Z., Klionsky D.J. Eaten alive: a history of macroautophagy. Nat. Cell Biol. 2010, 12:814-822.
5. Geng J., Klionsky D.J. The Atg8 and Atg12 ubiquitin-like conjugation systems in macroautophagy. EMBO Rep. 2008, 9:859-864.
6. Mizushima N., Yoshimori T., Levine B. Methods in mammalian autophagy research. Cell 2010, 140:313-326.
7. Ingham P.W. Zebrafish genetics and its implications for understanding vertebrate development. Hum. Mol. Genet. 1997, 6:1755-1760.
8. Wixon J. Featured organism: Danio rerio, the zebrafish. Yeast 2000, 17:225-231.
9. Liu S., Leach S.D. Zebrafish models for cancer. Annu. Rev. Pathol. 2011, 6:71-93.
10. Feitsma H., Cuppen E. Zebrafish as a cancer model. Mol. Cancer Res. 2008, 6:685-694.
11. Amatruda J.F., Shepard J.L., Stern H.M., Zon L.I. Zebrafish as a cancer model system. Cancer Cell 2002, 1:229-231.
12. Lam S.H., Gong Z. Modeling liver cancer using zebrafish: a comparative oncogenomics approach. Cell Cycle 2006, 5(6):573-577.
13. Lam S.H., Wu Y.L., Vega V.B., Miller L.D., Spitsbergen J., Tong Y., Zhan H., Govindarajan K.R., Lee S., Mathavan S., Murthy K.R., Buhler D.R., Liu E.T., Gong Z. Conservation of gene expression signatures between zebrafish and human liver tumors and tumor progression. Nat. Biotechnol. 2006, 24:73-75.
14. Stoletov K., Klemke R. Catch of the day: zebrafish as a human cancer model. Oncogene 2008, 27:4509-4520.
15. Flemingab A., Rubinszteina D.C. Zebrafish as a model to understand autophagy and its role in neurological disease. Biochim. Biophys. Acta 1812, 2010:520-526.
16. Dowling J.J., Low S.E., Busta A.S., Feldman E.L. Zebrafish MTMR14 is required for excitation-ontraction coupling, developmental motor function and the regulation of autophagy. Hum. Mol. Genet. 2010, 19:2668-2681.
17. Yabu T., Imamura S., Mizusawa N., Touhata K., Yamashita M. Induction of autophagy by amino acid starvation in fish cells. Mar. Biotechnol. (NY) 2012.
18. Y. Ding, X. Sun, X. Xu, TOR-autophagy signaling in adult zebrafish models of cardiomyopathy, Autophagy 8 (2012).
19. Z. Hu, J. Zhang, Q. Zhang, Expression pattern and functions of autophagy-related gene atg5 in zebrafish organogenesis, Autophagy 7 (2011).
20. He C., Bartholomew C.R., Zhou W., Klionsky D.J. Assaying autophagic activity in transgenic GFP-Lc3 and GFP-Gabarap zebrafish embryos. Autophagy 2009, 5:520-526.
21. Rabinowitz J.D., White E. Autophagy and metabolism. Science 2010, 330:1344-1348.
22. Rautou P.-E., Mansouri A., Lebrec D., Durand F., Valla D., Moreau R. Autophagy in liver diseases. J. Hepat. 2010, 53:1123-1134.
23. Takamura A., Komatsu M., Hara T., Sakamoto A., Kishi C., Waguri S., Eishi Y., Hino O., Tanaka K., Mizushima N. Autophagy-deficient mice develop multiple liver tumors. Genes Develop. 2011, 25:795-800.
24. Yue Z., Jin S., Yang C., Levine A.J., Heintz N. Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor. Proc. Natl. Acad. Sci. 2003, 100:15077.
25. Qu X., Yu J., Bhagat G., Furuya N., Hibshoosh H., Troxel A., Rosen J., Eskelinen E.L., Mizushima N., Ohsumi Y. Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene. J. Clin. Invest. 2003, 112:1809-1820.
26. Westerfield N. The Zebrafish Book 1995, University of Oregon Press, Eugene, USA, 1995.
27. He C., Klionsky D.J. Analyzing autophagy in zebrafish. Autophagy 2010, 6:642-644.
28. Kabeya Y., Mizushima N., Ueno T., Yamamoto A., Kirisako T., Noda T., Kominami E., Ohsumi Y., Yoshimori T. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 2000, 19:5720-5728.
29. Emelyanov A., Gao Y., Naqvi N.I., Parinov S. Trans-kingdom transposition of the maize dissociation element. Genetics 2006, 174:1095-1104.
30. Ellett F., Lieschke G.J. Zebrafish as a model for vertebrate hematopoiesis. Curr. Opin. Pharmacol. 2010, 10:563-570.
31. Kimura S., Noda T., Yoshimori T. Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Autophagy 2007, 3:452.
32. Mizushima N., Yamamoto A., Matsui M., Yoshimori T., Ohsumi Y. In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker. Mol. Biol. Cell 2004, 15:1101-1111.
33. Li Z., Huang X., Zhan H., Zeng Z., Li C., Spitsbergen J.M., Meierjohann S., Schartl M., Gong Z. Inducible and repressable oncogene-addicted hepatocellular carcinoma in Tet-on xmrk transgenic zebrafish. J. Hepatol. 2012, 56:419-425.
34. Nguyen A.T., Emelyanov A., Koh C.H.V., Spitsbergen J.M., Parinov S., Gong Z. An inducible krasV12 transgenic zebrafish model for liver tumorigenesis and chemical drug screening. Dis. Model. Mech. 2012, 5:63.
35. Nguyen A.T., Emelyanov A., Koh C.H.V., Spitsbergen J.M., Lam S.H., Mathavan S., Parinov S., Gong Z. A high level of liver-specific expression of oncogenic KrasV12 drives robust liver tumorigenesis in transgenic zebrafish. Dis. Model. Mech. 2011, 4:801.
36. Eskelinen E.L. The dual role of autophagy in cancer. Curr. Opin. Pharmacol. 2011, 11:294-300.
37. Gong Z., Koh C.H.V., Nguyen A.T., Zhan H., Li Z., Lam S.H., Spitsbergen J.M., Emelyanov A., Parinov S. The zebrafish model for liver carcinogenesis. Mol. Genet. Liver Neopl. 2011, 197-218.