Development and validation of a three-dimensional in vitro model for uterine leiomyoma and patient-matched myometrium

Fertility and Sterility

Volumn 97, Issue 6, 2012, Pages 1287-1293

  Malik, Minnie ^a   Catherino, William H ^a,b  

^a UNIFORMED SERVICES UNIVERSITY OF THE HEALTH SCIENCES   (United States)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

3D culture; extracellular matrix; myometrium; uterine leiomyoma

Indexed keywords

BETA1 INTEGRIN; COLLAGEN 1A1; COLLAGEN TYPE 1; DERMATOPONTIN; FIBRONECTIN; INTEGRIN; PROTEIN; TRANSFORMING GROWTH FACTOR BETA3; UNCLASSIFIED DRUG; VERSICAN;

APOPTOSIS; ARTICLE; CELL COMMUNICATION; CELL CULTURE; CELL STRUCTURE; CONTROLLED STUDY; DOWN REGULATION; EXTRACELLULAR MATRIX; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; HYSTERECTOMY; IMMUNOFLUORESCENCE; IN VITRO STUDY; MYOMETRIUM; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPINDLE CELL; THREE DIMENSIONAL CULTURE SYSTEM; UNIVERSITY HOSPITAL; UTERUS MYOMA; VALIDATION PROCESS; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE, TRANSFORMED; CHONDROITIN SULFATE PROTEOGLYCANS; COLLAGEN TYPE I; EXTRACELLULAR MATRIX PROTEINS; FEMALE; FIBRONECTINS; FLUOROIMMUNOASSAY; HUMANS; LEIOMYOMA; MYOMETRIUM; PRIMARY CELL CULTURE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSFORMING GROWTH FACTOR BETA3; UTERINE NEOPLASMS; VERSICANS;

EID: 84861632122     PISSN: 00150282     EISSN: 15565653     Source Type: Journal    
DOI: 10.1016/j.fertnstert.2012.02.037     Document Type: Article

References (36)

1. M. Malik, J. Norian, D. McCarthy-Keith, J. Britten, and W.H. Catherino Why leiomyomas are called fibroids: the central role of extracellular matrix in symptomatic women Semin Reprod Med 28 2010 169 179
2. W.H. Catherino Insights and advances in uterine leiomyomas Semin Reprod Med 28 2010 167 168
3. Lethaby A, Vollenhoven B. Fibroids (uterine myomatosis, leiomyomas). Clin Evid (Online). Published online January 22, 2011.
4. B. Weigelt, and M.J. Bissell Unraveling the microenvironmental influences on the normal mammary gland and breast cancer Semin Cancer Biol 18 2008 311 321
5. J. Lee, M.J. Cuddihy, and N.A. Kotov Three-dimensional cell culture matrices: state of the art Tissue Eng Part B Rev 14 2008 61 86
6. H.A. Adissu, E.K. Asem, and S.A. Lelièvre Three-dimensional cell culture to model epithelia in the female reproductive system Reprod Sci 14 2007 11 19
7. M. Malik, J. Webb, and W.H. Catherino Retinoic acid treatment of human leiomyoma cells transformed the cell phenotype to one strongly resembling myometrial cells Clin Endocrinol 69 2008 462 470
8. S.J. Gunst, and W. Zhang Actin cytoskeletal dynamics in smooth muscle: a new paradigm for the regulation of smooth muscle contraction Am J Physiol Cell Physiol 295 2008 C576 C587
9. T. Lecuit, and P.F. Lenne Cell surface mechanics and the control of cell shape, tissue patterns and morphogenesis Nat Rev Mol Cell Biol 8 2007 633 644
10. V.M. Weaver, A.H. Fischer, O.W. Peterson, and M.J. Bissell The importance of the microenvironment in breast cancer progression: recapitulation of mammary tumorigenesis using a unique human mammary epithelial cell model and a three-dimensional culture assay Biochem Cell Biol 74 1996 833 851
11. J. Debnath, and J.S. Brugge Modelling glandular epithelial cancers in three dimensional cultures Nat Rev 5 2005 675 688
12. M. Malik, and W.H. Catherino Novel method to characterize primary cultures of leiomyoma and myometrium with the use of confirmatory biomarker gene arrays Fertil Steril 87 2007 1166 1172
13. M.A. Wozniak, and P.J. Keely Use of three-dimensional collagen gels to study mechanotransduction in T47D breast epithelial cells Biol Proced Online 7 2005 144 161
14. M.W. Pfaffl A new mathematical model for relative quantification in real-time RT-PCR Nucleic Acids Res 29 2001 e45
15. F. Hertelendy, and T. Zakar Regulation of myometrial smooth muscle functions Curr Pharm Des 10 2004 2499 2517
16. L. Palmberg, and J. Thyberg Uterine smooth muscle cells in primary culture Cell Tissue Res 246 1986 253 262
17. W.H. Catherino, and M. Malik Uterine leiomyomas express a molecular pattern that lowers retinoic acid exposure Fertil Steril 87 2007 1388 1398
18. J.M. Norian, M. Malik, C.Y. Parker, D. Joseph, P.C. Leppert, and J.H. Segars Transforming growth factor β3 regulates the versican variants in the extracellular matrix-rich uterine leiomyomas Reprod Sci 16 2009 1153 1164
19. W.H. Catherino, M. Locastro, M. Gilden, J. Britten, and M. Malik Leiomyoma growth mediated by disruptions in injury response and angiogenesis Fertil Steril 96 2011 S51
20. M. Malik, D. Jardine, C.M. Owen, D. McCarthy-Keith, J.H. Segars, and W.H. Catherino Human leiomyoma cell proliferation and extracellular matrix expression is inhibited by integrin signaling Fertil Steril 92 2009 S2
21. D.S. Joseph, M. Malik, S. Nurudeen, and W.H. Catherino Myometrial cells undergo fibrotic transformation under the influence of transforming growth factor beta-3 Fertil Steril 93 2010 1500 1508
22. W.H. Catherino, C. Prupas, J.C. Tsibris, P.C. Leppert, M. Payson, and L.K. Nieman Strategy for elucidating differentially expressed genes in leiomyomata identified by microarray technology Fertil Steril 80 2003 282 290
23. J.C. Tsibris, J. Segars, D. Coppola, S. Mane, G.D. Wilbanks, and W.F. O'Brien Insights from gene arrays on the development and growth regulation of uterine leiomyomata Fertil Steril 78 2002 114 121
24. J.C. Tsibris, J. Segars, S. Enkemann, D. Coppola, G.D. Wilbanks, and W.F. O'Brien New and old regulators of uterine leiomyoma growth from screening with DNA arrays Fertil Steril 80 2003 279 281
25. T.M. Roth, C. Klett, and B.D. Cowan Expression profile of several genes in human myometrium and uterine leiomyoma Fertil Steril 87 2007 635 641
26. I.K. Dimitrova, J.K. Richer, M.C. Rudolph, N.S. Spoelstra, E.M. Reno, and T.M. Medina Gene expression profiling of multiple leiomyomata uteri and matched normal tissue from a single patient Fertil Steril 91 2009 2650 2663
27. S. Li, J. Lao, B.P. Chen, Y.S. Li, Y. Zhao, and J. Chu Genomic analysis of smooth muscle cells in 3-dimensional collagen matrix FASEB J 17 2003 97 99
28. K. Kuroda, O. Okamoto, and H. Shinkai Dermatopontin expression is decreased in hypertrophic scar and systemic sclerosis skin fibroblasts and is regulated by transforming growth factor-β1, interleukin-4, and matrix collagen J Invest Dermatol 112 1999 706 710
29. W.H. Catherino, P.C. Leppert, M.H. Stenmark, M. Payson, C. Potlog-Nahari, and L.K. Nieman Reduced dermatopontin expression is a molecular link between uterine leiomyomas and keloids Genes Chromosomes Cancer 40 2004 204 217
30. E. Levens, X. Luo, L. Ding, R.S. Williams, and N. Chegini Fibromodulin is expressed in leiomyoma and myometrium and regulated by gonadotropin-releasing hormone analogue therapy and TGF-beta through Smad and MAPK-mediated signalling Mol Hum Reprod 11 2005 489 494
31. R. Rogers, J. Norian, M. Malik, G. Christman, M. Abu-Asab, and F. Chen Mechanical homeostasis is altered in uterine leiomyoma Am J Obstet Gynecol 198 2008 474.e1 474.e11
32. S.K. Akiyama Integrins in cell adhesion and signaling Hum Cell 9 1996 181 186
33. A.L. Berrier, and K.M. Yamada Cell-matrix adhesion J Cell Physiol 213 2007 565 573
34. M.A. Schwartz Integrins and extracellular matrix in mechanotransduction Cold Spring Harb Perspect Biol 2 2010 a005066
35. D.G. Stupack The biology of integrins Oncology 21 2007 6 12
36. D.G. Stupack, X.S. Puente, S. Boutsaboualoy, C.M. Storgard, and D.A. Cheresh Apoptosis of adherent cells by recruitment of caspase-8 to unligated integrins J Cell Biol 155 2001 459 470