Silver-loaded biomimetic hydroxyapatite grafted poly(ε-caprolactone) composite nanofibers: A cytotoxicity study

Journal of Biomedical Nanotechnology

Volumn 8, Issue 1, 2012, Pages 125-132

  Nirmala, R ^a   Kang, Hyung Sub ^a   Park, Hye Min ^a   Navamathavan, R ^a   Jeong, In Soo ^b   Kim, Hak Yong ^a  

^a Chonbuk National University   (South Korea)

^b Korea Institute of Science and Technology Information (KISTI)   (South Korea)

Author keywords

Biomimetic hydroxyapatite; Cell culture; Composite nanofibers; Cytotoxicity; Electrospinning; PCL; Silver nanoparticles

Indexed keywords

BIOMEDICAL APPLICATIONS; BONE REGENERATION; CAPROLACTONE; COMPOSITE NANOFIBERS; CYTOTOXIC EFFECTS; ELECTROSPINNING PROCESS; ENERGY DISPERSIVE X-RAY; HUMAN CELLS; IN-VITRO; PCL; PROPERTIES OF COMPOSITES; SEM IMAGE; SILVER NANOPARTICLES; STRUCTURE AND THERMAL PROPERTIES; TRANSMISSION ELECTRON MICROSCOPY TEM;

ADHESION; BIOMIMETICS; CELL CULTURE; CYTOTOXICITY; ELECTROSPINNING; HYDROXYAPATITE; MEDICAL APPLICATIONS; NANOFIBERS; NANOPARTICLES; SCAFFOLDS (BIOLOGY); SCANNING ELECTRON MICROSCOPY; THERMOGRAVIMETRIC ANALYSIS; TRANSMISSION ELECTRON MICROSCOPY; X RAY DIFFRACTION;

SILVER;

BIOMIMETIC MATERIAL; HYDROXYAPATITE; NANOFIBER; NANOMATERIAL; NANOPARTICLE; POLYCAPROLACTONE; SILVER;

ARTICLE; BONE REGENERATION; CELL ADHESION; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; ELECTROSPINNING; HUMAN; HUMAN CELL; IN VITRO STUDY; MORPHOLOGY; OSTEOBLAST; ROENTGEN SPECTROSCOPY; SCANNING ELECTRON MICROSCOPY; THERMOGRAVIMETRY; TRANSMISSION ELECTRON MICROSCOPY; X RAY DIFFRACTION;

ANALYSIS OF VARIANCE; ANIMALS; BIOCOMPATIBLE MATERIALS; CATTLE; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; DURAPATITE; ELASTIC MODULUS; HUMANS; MATERIALS TESTING; NANOFIBERS; OSTEOBLASTS; POLYESTERS; POROSITY; SILVER; TISSUE SCAFFOLDS;

EID: 84861466201     PISSN: 15507033     EISSN: 15507041     Source Type: Journal    
DOI: 10.1166/jbn.2012.1359     Document Type: Article

References (23)

1. K. Fujihara, M. Kotaki, and S. Ramakrishan, Guided bone regeneration membrane made of polycaprolactone/calcium carbonate composite nano-fibers. Biomaterials 26, 4139 (2005).
2. F. Yang, S. K. Both, X. Yang, X. F. Walboomers, and J. A. Jansen, Development of an electrospun nano-apatite/PCL composite membrane for GTR/GBR application. Acta Biomaterialia 5, 3295 (2009).
3. H. W. Kim, J. H. Song, and H. E. Kim, Nanofiber Generation of Gelatin-hydroxyapatite biomimetics for guided tissue regeneration. Adv. Funct. Mater. 15, 1988 (2005).
4. Y. You, W. H. Park, B. M. Ko, and B. M. Min, Effects of PVA sponge containing chitooligosaccharide in the early stage of wound healing. J. Mater. Sci. Mater. Med. 15, 297 (2004).
5. M. D. Dhanaraju, D. Gopinath, M. R. Ahmed, R. Jayakumar, and C. Vamsadhara, Characterization of polymeric poly(epsiloncaprolactone) injectable implant delivery system for the controlled delivery of contraceptive steroids. J. Biomed. Mater. Res. A 76, 63 (2006).
6. C. Li, C. Vepari, H. J. Jin, H. Kim, and D. Kaplan, Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials 27, 3115 (2006).
7. S. Liao, W. Wang, M. Uo, S. Ohkawa, T. Akasaka, K. Tamura, F. Cui, and F. Watari, A three-layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane for guided tissue regeneration. Biomaterials 26, 7564 (2005).
8. J. R. Venugopal, P. Vadgama, T. S. S. Kumar, and S. Ramakrishna, Biocomposite nanofibres and osteoblasts for bone tissue engineering. Nanotechnology 18, 055101 (2007).
9. Y. Zhang, J. R. Venugopal, A. E. Turki, S. Ramakrishna, B. Su, and C. T. Lim, Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering. Biomaterials 29, 4314 (2008).
10. Z. C. Xing, W. P. Chae, J. Y. Baek, M. J. Choi, Y. Jung, and I. K. Kang, In vitro assessment of antibacterial activity and cytocompatibility of silver-containing phbv nanofibrous scaffolds for tissue engineering. Biomacromolecules 11, 1248 (2010).
11. R. Nirmala, K. T. Nam, D. K. Park, B. W. Il, R. Navamathavan, and H. Y. Kim, Structural, thermal, mechanical and bioactivity evaluation of silver-loaded bovine bone hydroxyapatite grafted poly(ε-caprolactone) nanofibers via electrospinning. Surf. Coat. Technol. 205, 174 (2010).
12. R. Nirmala, F. A. Sheikh, M. A. Kanjwal, J. H. Lee, S. J. Park, R. Navamathavan, and H. Y. Kim, Synthesis and characterization of bovine femur bone hydroxyapatite containing silver nanoparticles for the biomedical applications. J. Nanopart. Res. 13, 1917 (2010).
13. S. S. L. Sobhana, J. Sundaraseelan, S. Sekar, T. P. Sastry, and A. B. Mandal, Gelatin-chitosan composite capped gold nanoparticles: A matrix for the growth of hydroxyapatite. J. Nanopart. Res. 11, 333 (2009).
14. S. Yang, H. C. Man, W. Xing, and X. Zheng, Adhesion strength of plasma-sprayed hydroxyapatite coatings on laser gas-nitrided pure titanium. Surf. Coat. Technol. 203, 3116 (2009).
15. N. A. M. Barakat, M. S. Khil, A. M. Omran, F. A. Sheik, and H. Y. Kim, Extraction of pure natural hydroxyapatite from the bovine bones bio waste by three different methods. J. Mater. Proc. Technol. 209, 3408 (2009).
16. N. Rameshbabu, T. S. Sampath Kumar, T. G. Prabhakar, V. S. Sastry, K. V. G. K. Murty, and K. Prasad Rao, Antibacterial nanosized silver substituted hydroxyapatite: Synthesis and characterization. J. Biomed. Mater. Res. A 80, 581 (2007).
17. K. Nomura, S. Fujii, Y. Ohki, K. Awazu, M. Fujimaki, J. Tominaga, N. Fukuda, T. Hirakawa, and C. Rockstuhl, Fabrication of inert silver nanoparticles with a thin silica coating. Jpn. J. Appl. Phys. 47, 8641 (2008).
18. Y. Chen, X. Zheng, Y. Xie, C. Ding, H. Ruan, and C. Fan, Anti-bacterial and cytotoxic properties of plasma sprayed silver-containing HA coatings. J. Mater. Sci. Mater. Med. 19, 3603 (2008).
19. S. M. Magana, P. Quintana, D. H. Aguilar, J. A. Toledo, C. A. Chavez, M. A. Cortes, L. Leon, Y. F. Pelegrin, T. Lopez, and R. M. T. Sanchez, Antibacterial activity of montmorillonites modified with silver. J. Mol. Catal. A 281, 191 (2008).
20. G. Balasundaram, M. Sato, and T. J. Webster, Using hydroxyapatite nanoparticles and decreased crystallinity to promote osteoblast adhesion similar to functionalizing with RGD. Biomaterials 27, 2798 (2006).
21. iaccompanied by ERK 1/2 activation in human osteoblast cells. FEBS Lett. 581, 5929 (2007).
22. N. Miura and Y. Shinohara, Cytotoxic effect and apoptosis induction by silver nanoparticles in HeLa cells. Biochem. Biophys. Res. Commun. 390, 733 (2009).
23. G. Fotakis and J. A. Timbrell, In vitro cytotoxicity assays: Comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicology Lett. 160, 171 (2006).