Autoantibody detection using indirect immunofl uorescence on HEp-2 cells;Autoantikörpernachweis mittels indirekter Immunfluoreszenz an HEp-2-Zellen

LaboratoriumsMedizin

Volumn 36, Issue 3, 2012, Pages 135-141

  Sack, Ulrich ^a   Conrad, Karsten ^b   Csernok, Elena ^c   Frank, Ingrid ^d   Hiepe, Falk ^e   Krieger, Thorsten ^f   Kromminga, Arno ^g   Von Landenberg, Philipp ^h   Messer, Gerald ⁱ   Witte, Torsten ^j   Mierau, Rudolf ^k  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^c UNIVERSITY HOSPITAL SCHLESWIG HOLSTEIN   (Germany)

^d Labor Dr Tiller   (Germany)

^e CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^f UNIVERSITY OF HAMBURG   (Germany)

^g Labor Lademannbogen   (Germany)

^h JOHANNES GUTENBERG UNIVERSITY   (Germany)

ⁱ LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^j HANNOVER MEDICAL SCHOOL   (Germany)

^k Labor an der Rheumaklinik Aachen   (Germany)

more..

Author keywords

antinuclear antibodies; autoantibodies; autoimmune diseases; HEp 2 cells; immunofluorescence; laboratory diagnostics; methods; microscopy; standardization

Indexed keywords

AUTOANTIBODY;

ANTIBODY DETECTION; ANTIBODY SCREENING; ARTICLE; DIAGNOSTIC PROCEDURE; FLUORESCENCE MICROSCOPE; HEP 2 CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; PATHOLOGY; STATISTICAL ANALYSIS;

ANAS;

EID: 84861380309     PISSN: 03423026     EISSN: 14390477     Source Type: Journal    
DOI: 10.1515/labmed-2011-0016     Document Type: Article

References (10)

1. Holborow EJ, Weir DM, Johnson GD. A serum factor in lupus erythematosus with affinity for tissue nuclei. Br Med J 1957;2:732-4.
2. Hahon N, Eckert HL, Stewart J. Evaluation of cellular substrates for antinuclear antibody determinations. J Clin Microbiol 1975;2:42-5.
3. Richtlinie 98/79/EG des Europ ä ischen Parlaments und des Rates vom 27. Oktober 1998 ü ber In-vitro-Diagnostika. Amtsblatt der Europ ä ischen Gemeinschaften L 331/1, 7.12.98 DE.
4. Sack U, Conrad K, Csernok E, Frank I, Haas M, Krieger T, et al. Standardization of Autoimmune Diagnostics in Germany: Activities of the German Group in the European Autoimmune Standardization Initiative. Ann N Y Acad Sci 2007;1109:31-6.
5. Kang I, Siperstein R, Quan T, Breitenstein ML. Utility of age, gender, ANA titer and pattern as predictors of anti-ENA and -dsDNA antibodies. Clin Rheumatol 2004;23:509-15.
6. Hiemann R, Conrad K, Roggenbuck D, Gro ßmann K, Michel J, Anderer U, et al. Die HEp-2-Zelle als Target für multiparametrische Autoantik örperanalytik-Automatisierung und Standardisierung. In: Conrad K, Lehmann W, Sack U, Schedler U, editors. Multiparameteranalytik-Methoden, Applikationen, Perspektiven. Lengerich: Pabst Science Publishers, Lengerich, 2008:98-117.
7. Kahn E, Vejux A, Méné trier F, Maiza C, Hammann A, Sequeira-Le Grand A, et al. Analysis of CD36 expression on human monocytic cells and atherosclerotic tissue sections with quantum dots: Investigation by flow cytometry and spectral imaging microscopy. Anal Quant Cytol Histol 2006;28: 14-26.
8. Hiemann R, Hilger N, Weigert M, Sack U, Michel J, Andere U. Image based description of immunofluorescence on HEp-2 cells. Cytometry Part A 2007;71A:60.
9. Sack U, Knoechner S, Warschkau H, Pigla U, Emmrich F, Kamprad M. Computer-assisted classification of HEp-2 immunofluorescence patterns in autoimmune diagnostics. Autoimmun Rev 2003;2:298-304.
10. Soda P. Early Experiences in the Staining Pattern Classification of HEp-2 Slides. Proceedings of the Twentieth IEEE International Symposium on Computer-Based Medical Systems (CBMS ' 07), 2007.