Murine 3T3-L1 Adipocyte cell differentiation model: Validated reference genes for qPCR gene expression analysis

PLoS ONE

Volumn 7, Issue 5, 2012, Pages

  Arsenijevic, Tatjana ^a   Grégoire, Françoise ^a   Delforge, Valérie ^b   Delporte, Christine ^a   Perret, Jason ^a  

^a UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^b Nutrisub ASBL   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA;

ADIPOCYTE; ANIMAL CELL; ARTICLE; ATP SYNTHASE H+ TRANSPORTING MITOCHONDRIAL F1 COMPLEX BETA SUBUNIT GENE; BETA ACTIN GENE; CELL CLONE; CELL DIFFERENTIATION; CELL EXPANSION; CELL STRAIN 3T3; DEVELOPMENTAL STAGE; GENE; GENE EXPRESSION; GENETIC STABILITY; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE GENE; MITOSIS; MOUSE; NON POU DOMAIN CONTAINING OCTAMER BINDING PROTEIN GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REFERENCE GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RIBOSOMAL PROTEIN L13A GENE; VALIDATION STUDY; YWHAZ GENE;

3T3-L1 CELLS; ADIPOCYTES; ANIMALS; CELL DIFFERENTIATION; GENE EXPRESSION; MICE; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS;

MURINAE;

EID: 84861316382     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0037517     Document Type: Article

References (37)

1. Waki H, Tontonoz P, (2007): Endocrine functions of adipose tissue. Annu.Rev Pathol. 2: 31-56.
2. Visscher TL, Seidell JC, Molarius A, van der KD, Hofman A, Witteman JC, (2001): A comparison of body mass index, waist-hip ratio and waist circumference as predictors of all-cause mortality among the elderly: the Rotterdam study. Int.J Obes.Relat Metab Disord. 25: 1730-1735.
3. Farmer SR, (2006): Transcriptional control of adipocyte formation. Cell Metab 4: 263-273.
4. MacDougald OA, Lane MD, (1995): Transcriptional regulation of gene expression during adipocyte differentiation. Annu.Rev.Biochem. 64: 345-373.
5. Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, et al. (2004): Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques 37: 112-119.
6. Dheda K, Huggett JF, Chang JS, Kim LU, Bustin SA, et al. (2005): The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal.Biochem. 344: 141-143.
7. Hendriks-Balk MC, Michel MC, Alewijnse AE, (2007): Pitfalls in the normalization of real-time polymerase chain reaction data. Basic Res Cardiol. 102: 195-197.
8. MacDougald OA, Lane MD, (1995): Adipocyte differentiation. When precursors are also regulators. Curr.Biol. 5: 618-621.
9. Poulos SP, Dodson MV, Hausman GJ, (2010): Cell line models for differentiation: preadipocytes and adipocytes. Experimental Biology and Medicine 235: 1185-1193.
10. Cowherd RM, Lyle RE, McGehee J, (1999): Molecular regulation of adipocyte differentiation. Seminars in Cell & Developmental Biology 10: 3-10.
11. Ntambi JM, Young-Cheul K, (2000): Adipocyte Differentiation and Gene Expression. The Journal of Nutrition 130: 3122S-3126S.
12. Bustin SA, (2002): Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol 29: 23-39.
13. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, et al. (2002): Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3: RESEARCH0034-1-RESEARCH0034-11.
14. Pfaffl MW, (2001): A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29: e45.
15. Bustin SA, (2010): Why the need for qPCR publication guidelines?-The case for MIQE. Methods 50: 217-226.
16. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, et al. (2009): The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin.Chem. 55: 611-622.
17. Andersen CL, Jensen JL, Orntoft TF, (2004): Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64: 5245-5250.
18. Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP, (2004): Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol.Lett. 26: 509-515.
19. Stern-Straeter J, Bonaterra GA, Hormann K, Kinscherf R, Goessler UR, (2009): Identification of valid reference genes during the differentiation of human myoblasts. BMC.Mol Biol. 10: 66-74.
20. Stephens AS, Stephens SR, Morrison NA, (2011): Internal control genes for quantitative RT-PCR expression analysis in mouse osteoblasts, osteoclasts and macrophages. BMC.Res.Notes 4: 410-419.
21. Radonic A, Thulke S, Mackay IM, Landt O, Siegert W, et al. (2004): Guideline to reference gene selection for quantitative real-time PCR. Biochemical and Biophysical Research Communications 313: 856-862.
22. Maess MB, Sendelbach S, Lorkowski S, (2010): Selection of reliable reference genes during THP-1 monocyte differentiation into macrophages. BMC.Mol Biol. 11: 90.
23. Ferguson BS, Nam H, Hopkins RG, Morrison RF, (2010): Impact of reference gene selection for target gene normalization on experimental outcome using real-time qRT-PCR in adipocytes. PLoS.One. 5: e15208.
24. de Jonge HJ, Fehrmann RS, de Bont ES, Hofstra RM, Gerbens F, et al. (2007): Evidence based selection of housekeeping genes. PLoS.One. 2: e898.
25. Guenin S, Mauriat M, Pelloux J, Van Wuytswinkel O, Bellini C, et al. (2009): Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references. J Exp.Bot. 60: 487-493.
26. Bruge F, Venditti E, Tiano L, Littarru GP, Damiani E, (2011): Reference gene validation for qPCR on normoxia- and hypoxia-cultured human dermal fibroblasts exposed to UVA: Is +{pipe}-actin a reliable normalizer for photoaging studies? Journal of Biotechnology 156: 153-162.
27. Derveaux S, Vandesompele J, Hellemans J, (2010): How to do successful gene expression analysis using real-time PCR. Methods 50: 227-230.
28. Sugden K, Pariante CM, McGuffin P, Aitchison KJ, D'Souza UM, (2010): Housekeeping gene expression is affected by antidepressant treatment in a mouse fibroblast cell line. J Psychopharmacol. 24: 1253-1259.
29. Gabrielsson BG, Karlsson AC, Lönn M, Olofsson LE, Johansson JM, et al. (2004): Molecular characterization of a local sulfonylurea system in human adipose tissue. Molecular and Cellular Biochemistry 258: 65-71.
30. Gorzelniak K, Janke J, Engeli S, Sharma AM, (2001): Validation of Endogenous Controls for Gene Expression Studies in Human Adipocytes and Preadipocytes. Horm Metab Res 33: 625, 627.
31. Catalan V, Gomez-Ambrosi J, Rotellar F, Silva C, Rodriguez A, et al. (2007): Validation of Endogenous Control Genes in Human Adipose Tissue: Relevance to Obesity and Obesity-associated Type 2 Diabetes Mellitus. Horm Metab Res 39: 495, 500.
32. Barroso I, Benito B, Garci-Jimenez C, Hernandez A, Obregon MJ, et al. (1999): Norepinephrine, tri-iodothyronine and insulin upregulate glyceraldehyde-3-phosphate dehydrogenase mRNA during Brown adipocyte differentiation. Eur J Endocrinol 141: 169-179.
33. Kim I, Yang D, Tang X, Carroll J, (2011): Reference gene validation for qPCR in rat carotid body during postnatal development. BMC Research Notes 4: 440.
34. Solanas M, Moral R, Escrich E, (2001): Unsuitability of Using Ribosomal RNA as Loading Control for Northern Blot Analyses Related to the Imbalance between Messenger and Ribosomal RNA Content in Rat Mammary Tumors. Analytical Biochemistry 288: 99-102.
35. Samaras K, Botelho N, Chisholm D, Lord R, (2010): Subcutaneous and Visceral Adipose Tissue FTO Gene Expression and Adiposity, Insulin Action, Glucose Metabolism, and Inflammatory Adipokines in Type 2 Diabetes Mellitus and in Health. Obesity Surgery 20: 108-113.
36. Jacobs C, Onnockx S, Vandenbroere I, Pirson I, (2004): Endogenous SHIP2 does not localize in lipid rafts in 3T3-L1 adipocytes. FEBS Letters 565: 70-74.
37. Maroufi A, Van Bockstaele E, De Loose M, (2010): Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR. BMC Molecular Biology 11: 15.