One-step labeling of degenerative neurons in unfixed brain tissue samples using Fluoro-Jade C

Journal of Neuroscience Methods

Volumn 208, Issue 1, 2012, Pages 40-43

  Gu, Qiang ^a   Schmued, Larry C ^a   Sarkar, Sumit ^a   Paule, Merle G ^a   Raymick, Bryan ^a  

^a NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH   (United States)

Author keywords

Fluorescence labeling; Fluoro Jade; Neurodegeneration; Neuronal death

Indexed keywords

DYE; FLUORO JADE C; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; CELL LABELING; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; INCUBATION TIME; MICROSCOPY; NERVE DEGENERATION; NONHUMAN; PH; PRIORITY JOURNAL; RAT;

ANIMALS; BRAIN; FLUORESCENT DYES; NEURODEGENERATIVE DISEASES; ORGANIC CHEMICALS; RATS; RATS, SPRAGUE-DAWLEY; STAINING AND LABELING; TISSUE FIXATION;

EID: 84861213655     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2012.04.012     Document Type: Article

References (15)

1. Anderson K.J., Miller K.M., Fugaccia I., Scheff S.W. Regional distribution of Fluoro-Jade B staining in the hippocampus following traumatic brain injury. Exp Neurol 2005, 193:125-130.
2. Chidlow G., Wood J.P., Sarvestani G., Manavis J., Casson R.J. Evaluation of Fluoro-Jade C as a marker of degenerating neurons in the rat retina and optic nerve. Exp Eye Res 2009, 88:426-437.
3. de Olmos J.S., Beltramino C.A., de Olmos de Lorenzo S. Use of an amino-cupric-silver technique for the detection of early and semiacute neuronal degeneration caused by neurotoxicants, hypoxia, and physical trauma. Neurotoxicol Teratol 1994, 16:545-561.
4. Emgård M., Karlsson J., Hansson O., Brundin P. Patterns of cell death and dopaminergic neuron survival in intrastriatal nigral grafts. Exp Neurol 1999, 160:279-288.
5. Garcia J.H., Kamijyo Y. Cerebral infarction: evolution of histopathological changes after occlusion of a middle cerebral artery in primates. J Neuropathol Exp Neurol 1974, 33:408-421.
6. Gavrieli Y., Sherman Y., Ben-Sasson S.A. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 1992, 119:493-501.
7. Kokaia Z., Andsberg G., Yan Q., Lindvall O. Rapid alterations of BDNF protein levels in the rat brain after focal ischemia: evidence for increased synthesis and anterograde axonal transport. Exp Neurol 1998, 154:289-301.
8. Lillie R.D. Histopathologic technical and practical histochemistry 1976, McGraw-Hill, New York. 4th ed.
9. Pérez-González R., Antequera D., Vargas T., Spuch C., Bolós M., Carro E. Leptin induces proliferation of neuronal progenitors and neuroprotection in a mouse model of Alzheimer's disease. J Alzheimers Dis 2011, 24(Suppl. 2):17-25.
10. Sarkar S., Schmued L. Fluoro-Jade dyes: fluorochromes for the histochemical localization of degenerative neurons. Fundamental neuropathology for pathologists and toxicologists: principles and techniques 2011, 171-179. John Wiley & Sons, Hoboken.
11. Schmued L.C., Albertson C., Slikker W. Fluoro-Jade: a novel fluorochrome for the sensitive and reliable histochemical localization of neuronal degeneration. Brain Res 1997, 751:37-46.
12. Schmued L.C., Hopkins K.J. Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration. Brain Res 2000, 874:123-130.
13. Schmued L.C., Stowers C.C., Scallet A.C., Xu L. Fluoro-Jade C results in ultra high resolution and contrast labeling of degenerating neurons. Brain Res 2005, 1035:24-31.
14. Siesjo B.K. Cell damage in the brain: a speculative synthesis. J Cereb Blood Flow Metab 1981, 1:155-185.
15. Yuan J., Horvitz H.R. A first insight into the molecular mechanisms of apoptosis. Cell 2004, 116(2 Suppl.):53-56.