Development of a thermostable β-glucuronidase-based reporter system for monitoring gene expression in hyperthermophiles

Biotechnology and Bioengineering

Volumn 109, Issue 7, 2012, Pages 1881-1886

  Honarbakhsh, Maryam ^a   Villafane, Aramis A ^b   Ruhl, Ilona ^c   Sannino, David ^a   Bini, Elisabetta ^a  

^a RUTGERS UNIVERSITY   (United States)

^b UNIVERSITY OF PUERTO RICO   (Puerto Rico)

^c PRINCETON UNIVERSITY   (United States)

Author keywords

glucuronidase; Archaea; Reporter gene; Sulfolobus

Indexed keywords

ARCHAEA; CODING GENES; GENETIC STUDIES; GLUCURONIDASE; HYPERTHERMOPHILES; IN-VIVO; MESOPHILIC; PROOF OF CONCEPT; REPORTER GENE; REPORTER SYSTEMS; SULFOLOBUS; SULFOLOBUS SOLFATARICUS; TEMPERATURE RANGE;

GENE EXPRESSION;

PLANTS (BOTANY);

BETA GLUCURONIDASE;

ARTICLE; BACTERIAL STRAIN; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; FUSION GENE; GENE AMPLIFICATION; GENE EXPRESSION REGULATION; HYPERTHERMOPHILE; IN VIVO STUDY; MOLECULAR CLONING; NONHUMAN; PH MEASUREMENT; PLASMID; REPORTER GENE; SULFOLOBUS SOLFATARICUS; THERMOSTABILITY;

CLONING, MOLECULAR; GENE EXPRESSION PROFILING; GENES, REPORTER; GLUCURONIDASE; PROMOTER REGIONS, GENETIC; PROTEIN STABILITY; RECOMBINANT PROTEINS; SULFOLOBUS SOLFATARICUS; TEMPERATURE;

SULFOLOBUS; SULFOLOBUS SOLFATARICUS;

EID: 84861190252     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24432     Document Type: Article

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