Comparison of the Cytobrush ®, dermatological curette and oral CDx ® brush test as methods for obtaining samples of RNA for molecular analysis of oral cytology

Cytopathology

Volumn 23, Issue 3, 2012, Pages 192-197

  Reboiras Lopez M D ^a   Perez Sayans M ^a   Somoza Martin J M ^a   Gayoso Diz, P ^b   Barros Angueira, F ^c   Gandara Rey J M ^a   Garcia Garcia A ^a  

^a UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

^b HOSPITAL CLÍNICO UNIVERSITARIO   (Spain)

^c University Hospital of Santiago de Compostela   (Spain)

Author keywords

Curette; Cytobrush; Oral CDx; Oral exfoliative cytology; Oral squamous cell carcinoma; Quantitative real time polymerase chain reaction; RNA

Indexed keywords

ABELSON KINASE; RNA;

ADULT; ARTICLE; BIOPSY DEVICE; CLINICAL EFFECTIVENESS; COMPARATIVE STUDY; CONTROLLED STUDY; DEMOGRAPHY; EARLY DIAGNOSIS; FEMALE; GENE EXPRESSION; HUMAN; HUMAN EXPERIMENT; HUMAN TISSUE; MALE; MEDICAL RESEARCH; NON INVASIVE PROCEDURE; NORMAL HUMAN; ORAL BIOPSY; ORAL EXFOLIATIVE CYTOLOGY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA ANALYSIS; SAMPLING; SMOKING;

ADULT; CARCINOMA, SQUAMOUS CELL; CYTOLOGICAL TECHNIQUES; EARLY DETECTION OF CANCER; FEMALE; GENE DOSAGE; GENES, ABL; GENETIC MARKERS; HUMANS; MALE; MOUTH MUCOSA; REAL-TIME POLYMERASE CHAIN REACTION; RNA; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, RNA; SMOKING; YOUNG ADULT;

EID: 84861137292     PISSN: 09565507     EISSN: 13652303     Source Type: Journal    
DOI: 10.1111/j.1365-2303.2011.00860.x     Document Type: Article

References (29)

1. International agency for research on cancer World Health Organization: the GLOBOCAN 2008 database, 2010. [].
2. Navone R, Burlo P, Pich A et al. The impact of liquid-based oral cytology on the diagnosis of oral squamous dysplasia and carcinoma. Cytopathology 2007;18:356-60.
3. Sciubba JJ. Improving detection of precancerous and cancerous oral lesions. Computer-assisted analysis of the oral brush biopsy. U.S. Collaborative OralCDx Study Group. J Am Dent Assoc 1999;130:1445-57.
4. Driemel O, Kunkel M, Hullmann M et al. Diagnosis of oral squamous cell carcinoma and its precursor lesions. J Dtsch Dermatol Ges 2007;5:1095-100.
5. Navone R, Marsico A, Reale I et al. Usefulness of oral exfoliative cytology for the diagnosis of oral squamous dysplasia and carcinoma. Minerva Stomatol 2004;53:77-86.
6. Navone R. Cytology of the oral cavity: a re-evaluation. Pathologica 2009;101:6-8.
7. Kuffer R, Lombardi T. Premalignant lesions of the oral mucosa. A discussion about the place of oral intraepithelial neoplasia (OIN). Oral Oncol 2002;38:125-30.
8. Epstein JB, Zhang L, Rosin M. Advances in the diagnosis of oral premalignant and malignant lesions. J Can Dent Assoc 2002;68:617-21.
9. Acha A, Ruesga MT, Rodriguez MJ, Martinez de Pancorbo MA, Aguirre JM. Applications of the oral scraped (exfoliative) cytology in oral cancer and precancer. Med Oral Patol Oral Cir Bucal 2005;10:95-102.
10. Diniz-Freitas M, Garcia-Garcia A, Crespo-Abelleira A, Martins-Carneiro JL, Gandara-Rey JM. Applications of exfoliative cytology in the diagnosis of oral cancer. Med Oral 2004;9:355-61.
11. Spivack SD, Hurteau GJ, Jain R et al. Gene-environment interaction signatures by quantitative mRNA profiling in exfoliated buccal mucosal cells. Cancer Res 2004;64:6805-13.
12. Perez-Sayans M, Somoza-Martin J, Barros-Angueira F et al. Exfoliative cytology for diagnosing oral cancer. Biotech Histochem 2009;25:1-11.
13. Alberts B, Bray D, Hopkin K et al. Del DNA a la proteína: cómo leen las células el genoma. In: Introducción a la Biología Celular., 2nd edn. Alberts B, Bray D, Hopkin K et al., (eds). Editorial Médica Paramericana S.A.; Buenos Aires 2007: pp. 229-66.
14. Mehrotra R, Gupta A, Singh M, Ibrahim R. Application of cytology and molecular biology in diagnosing premalignant or malignant oral lesions. Mol Cancer 2006;5:11-20.
15. Ballantyne J. Validity of messenger RNA expression analyses of human saliva. Clin Cancer Res 2007;13:1350-1.
16. Laine ML, Farre MA, Crusius JB, van Winkelhoff AJ, Pena AS. The mouthwash: a non-invasive sampling method to study cytokine gene polymorphisms. J Periodontol 2000;71:1315-8.
17. Ogden GR, Cowpe JG, Green M. Cytobrush and wooden spatula for oral exfoliative cytology. A comparison. Acta Cytol 1992;36:706-10.
18. Driemel O, Kunkel M, Hullmann M et al. Performance of conventional oral brush biopsies. HNO 2008;56:205-10.
19. Mollaoglu N, Wilson MJ, Cowpe JG. Extraction of DNA from oral cytological samples by scraping and smear method suitable for restriction site mutation analysis: a pilot study. Diagn Cytopathol 2001;25:83-5.
20. Navone R, Pentenero M, Rostan I et al. Oral potentially malignant lesions: first-level micro-histological diagnosis from tissue fragments sampled in liquid-based diagnostic cytology. J Oral Pathol Med 2008;37:358-63.
21. Kosicki DM, Riva C, Pajarola GF, Burkhardt A, Gratz KW. OralCDx brush biopsy - a tool for early diagnosis of oral squamous cell carcinoma. Schweiz Monatsschr Zahnmed 2007;117:227.
22. Trullenque-Eriksson A, Munoz-Corcuera M, Campo-Trapero J, Cano-Sanchez J, Bascones-Martinez A. Analysis of new diagnostic methods in suspicious lesions of the oral mucosa. Med Oral Patol Oral Cir Bucal 2009;14:E210-6.
23. Mehrotra R, Hullmann M, Smeets R, Reichert TE, Driemel O. Oral cytology revisited. J Oral Pathol Med 2009;38:1616.
24. Spira A, Beane J, Schembri F et al. Noninvasive method for obtaining RNA from buccal mucosa epithelial cells for gene expression profiling. BioTechniques 2004;36:484-7.
25. Schwartz JL, Panda S, Beam C, Bach LE, Adami GR. RNA from brush oral cytology to measure squamous cell carcinoma gene expression. J Oral Pathol Med 2008;37:70-7.
26. Klaassen I, Copper MP, Brakenhoff RH, Smeets SJ, Snow GB, Braakhuis BJ. Exfoliated oral cells messenger RNA: suitability for biomarker studies. Cancer Epidemiol Biomarkers Prev 1998;7:469-72.
27. Perez-Sayans M, Reboiras-Lopez MD, Somoza-Martin JM et al. Measurement of ATP6V1C1 expression in brush cytology samples as a diagnostic and prognostic marker in oral squamous cell carcinoma. Cancer Biol Ther 2010;9:1-8.
28. Pentenero M, Giaretti W, Navone R et al. DNA aneuploidy and dysplasia in oral potentially malignant disorders: association with cigarette smoking and site. Oral Oncol 2009;45:887-90.
29. Habis AH, Vernon SD, Lee DR, Verma M, Unger E. Molecular quality of exfoliated cervical cells: implications for molecular epidemiology and biomarker discovery. Cancer Epidemiol Biomarkers Prev 2004;13:492-6.