Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Applied and Environmental Microbiology

Volumn 78, Issue 10, 2012, Pages 3759-3762

  Waters, Deborah M ^a,c,e   Murray, Patrick G ^a,b   Miki, Yuta ^c   Martínez, Angel T ^c   Tuohy, Maria G ^a   Faulds, Craig B ^c,d  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

^b Athletic Rehabilitation Therapy Ireland   (Ireland)

^c CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^d VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^e UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYL ESTERASE; HIGHER TEMPERATURES; OVER-EXPRESSION; POTENTIAL APPLICATIONS; TALAROMYCES EMERSONII;

ENZYMES; ESCHERICHIA COLI; ESTERS; GENE ENCODING;

CLONING;

ACETYLESTERASE; ACETYLXYLAN ESTERASE;

BIOFUEL; BIOMASS; CLONAL GROWTH; COLIFORM BACTERIUM; ENZYME ACTIVITY; FUNGUS; HIGH TEMPERATURE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PH; SEQUENCE ALIGNMENT; TALAROMYCES; TEMPERATURE;

ACETYLESTERASE; AMINO ACID SEQUENCE; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; TALAROMYCES; TEMPERATURE;

ESCHERICHIA COLI; TALAROMYCES EMERSONII;

EID: 84861125185     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.05659-11     Document Type: Article

References (23)

1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Altschul SF, et al. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
3. Biely P, Wong KKY, Suckling ID, Spániková S. 2003. Transacetylations to carbohydrates catalyzed by acetylxylan esterase in the presence of organic solvent. BBA Gen. Sub. 1623:62-71.
4. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
5. Chesson A, Gordon AH, Lomax JA. 1983. Substituent groups linked by alkali-labile bonds to arabinose and xylose residues of legume, grass and cereal straw cell walls and their fate during digestion by rumen microorganisms. J. Sci. Food Agric. 34:1330-1340.
6. Chomczynski P, Sacchi N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
7. Chung H-J, Park S-M, Kim H-R, Yang M-S, Kim D-H. 2002. Cloning the gene encoding acetyl xylan esterase from Aspergillus ficuum and its expression in Pichia pastoris. Enzyme. Microb. Tech. 31:384-391.
8. Coughlan M, Ljungdahl L. 1988. Comparative biochemistry of fungal and bacterial cellulolytic enzyme systems, p 11-30. In Aubert J-P, Beguin P, Millet J (ed), Biochemistry and genetics of cellulose degradation. Academic Press, London, United Kingdom.
9. Elving PJ, Markowitz JM, Rosenthal I. 1956. Preparation of buffer systems of constant ionic strength. Anal. Chem. 28:1179-1180.
10. Folan MA, Coughlan MP. 1979. The saccharifying ability of the cellulase complex of Talaromyces emersonii and comparison with that of other fungal species. Int. J. Biochem. 10:505.
11. Higgins DG. 1994. CLUSTAL W: multiple alignment of DNA and protein sequences. Methods Mol. Biol. 25:307-318.
12. Hu X, Heath C, Taylor M, Tuffin M, Cowan D. 2012. A novel, extremely alkaliphilic and cold-active esterase from Antarctic desert soil. Extremophiles 16:79-86.
13. Huang Y-C, Chen G-H, Chen Y-F, Chen W-L, Yang C-H. 2010. Heterologous expression of thermostable acetylxylan esterase gene from Thermobifida fusca and its synergistic action with xylanase for the production of xylooligosaccharides. Biochem. Biophys. Res. Commun. 400:718-723.
14. Johnson KG, Fontana JD, MacKenzie CR. 1988. Measurement of acetylxylan esterase in Streptomyces. Methods Enzymol. 68:551-560.
15. Krell HW, Sandermann H. 1984. Plant biochemistry of xenobiotics. Eur. J. Biochem. 143:57-62.
16. Moloney AP, Considine PJ, Coughlan MP. 1983. Cellulose hydrolysis by the cellulases produced by Talaromyces emersonii when grown on different inducing substrates. Biotechnol. Bioeng. 25:1169-1173.
17. Moloney AP, McCrae SI, Wood TM, Coughlan MP. 1985. Isolation and characterization of the 1,4-β-D-glucan glucanohydrolases of Talaromyces emersonii. Biochem. J. 225:365-374.
18. Moure A, Gullón P, Domínguez H, Parajó JC. 2006. Advances in the manufacture, purification and applications of xylo-oligosaccharides as food additives and nutraceuticals. Process Biochem. 41:1913-1923.
19. Murray PG, Collins CM, Grassick A, Tuohy MG. 2003. Molecular cloning, transcriptional, and expression analysis of the first cellulase gene (cbh2), encoding cellobiohydrolase II, from the moderately thermophilic fungus Talaromyces emersonii and structure prediction of the gene product. Biochem. Biophys. Res. Commun. 301:280-286.
20. Raeder U, Broda P. 1985. Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1:17-20.
21. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
22. Sedlmeyer FB. 2011. Xylan as by-product of biorefineries: characteristics and potential use for food applications. Food Hydrocolloids 25:1891-1898.
23. Tuohy MG, Coughlan MP. 1992. Production of thermostable xylandegrading enzymes by Talaromyces emersonii. Bioresource Technol. 39: 131-137.