Comparison of three methods of DNA extraction from human bones with different degrees of degradation

International Journal of Legal Medicine

Volumn 126, Issue 1, 2012, Pages 173-178

  Jakubowska, Joanna ^a,b   Maciejewska, Agnieszka ^b   Pawłowski, Ryszard ^b,c  

^a UNIVERSITY OF GDA SK   (Poland)

^b MEDICAL UNIVERSITY OF GDANSK   (Poland)

^c INSTITUTE OF FORENSIC RESEARCH   (Poland)

Author keywords

Bones; Crystal aggregates; DNA extraction; Inhibition; Real time PCR; Total demineralization

Indexed keywords

DNA;

ARTICLE; BONE; CHEMISTRY; COMPARATIVE STUDY; FEMALE; FORENSIC GENETICS; HISTOLOGY; HUMAN; ISOLATION AND PURIFICATION; MALE; METHODOLOGY; MULTIPLEX POLYMERASE CHAIN REACTION; PHYSICAL ANTHROPOLOGY; POLYMERASE CHAIN REACTION; TIME;

BONE AND BONES; DECALCIFICATION TECHNIQUE; DNA; FEMALE; FORENSIC ANTHROPOLOGY; FORENSIC GENETICS; HUMANS; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION; TIME FACTORS;

EID: 84861118873     PISSN: 09379827     EISSN: 14371596     Source Type: Journal    
DOI: 10.1007/s00414-011-0590-5     Document Type: Article

References (19)

1. Zupanic Pajnic I, Gornjak Pogorelc B, Balazic J (2010) Molecular genetic identification of skeletal remains from the SecondWorldWar Konfin I mass grave in Slovenia. Int J Legal Med 124:307-317.
2. Budowle B, Bieber F, Eisenberg A (2005) Forensic aspects of mass disasters: Strategic considerations for DNA-based human identification. Leg Med 7:230-243. (Pubitemid 40828862)
3. Holland MM, Cave CA, Holland CA, Bille TW(2003) Development of a quality, high throughput DNA analysis procedure for skeletal samples to assist with the identification of victims from the World Trade Center attacks. Croat Med J 44:264-272. (Pubitemid 36828142)
4. Primorac D (2004) The role of DNA technology in identification of skeletal remains discovered in mass graves. Forensic Sci Int 146S:163-164.
5. Bogdanowicz W, Allen M, Branicki W et al (2009) Genetic identification of putative remains of the famous astronomer Nicolaus Copernicus. PNAS 106:12279-12282.
6. Coble MD, Loreille OM, Wadhams MJ et al (2009) Mystery solved: the identification of the two missing Romanov children using DNA analysis. PLoS ONE 4(3):e4838. doi:10.1371/journal. pone.0004838.
7. Gill P, Ivanov PL, Kimpton C et al (1994) Identification of the remains of the Romanov family by DNA analysis. Nat Genetics 6:130-135. (Pubitemid 24188592)
8. Mulligan C (2005) Isolation and analysis of DNA from archaeological, clinical, and natural history specimen. Methods Enzymol 395:87-103. (Pubitemid 40754364)
9. Rohland N, Hofreiter M (2007) Ancient DNA extraction from bones and teeth. Nat Protoc 2:1756-1762.
10. Nilsson M, Possnert G, Edlund H et al (2010) Analysis of the putative remains of a European patron saint-St. Birgitta. PLoS ONE 5(2):e8986. doi:10.1371/journal.pone.0008986.
11. Salamon M, Tuross N, Arensburg B, Weiner S (2005) Relatively well preserved DNA is present in the crystal aggregates of fossil bones. PNAS 102:13783-13788. (Pubitemid 41377656)
12. Weiner S, Price P (1986) Disaggregation of bones into crystals. Calcif Tissue Int 39:365-375.
13. Hochmeister M, Budowle B (1991) Typing of DNA extracted from compact bone tissue from human remains. J Forensic Sci 36:1649-1661.
14. Loreille O, Diegoli TM, Irwin JA et al (2007) High efficiency DNA extraction from bone by total demineralization. Forensic Sci Int Genet 1:191-195. (Pubitemid 46760405)
15. Davoren J, Vanek D, Konjhodzić R et al (2007) Highly effective DNA extraction method for nuclear short tandem repeat testing of skeletal remains from mass graves. Croat Med J 48:478-485. (Pubitemid 47330048)
16. Putkonen MT, Palo JU, Cano JM et al (2010) Factors affecting the STR amplification success in poorly preserved bone samples. Investig Genet 1:9.
17. Rohland N, Hofreiter M (2007) Comparison and optimization of ancient DNA extraction. Biotechniques 42:343-352.
18. Schwark T, Heinrich A, Preuße-Prange A, von Wurmb-Schwark N (2010) Reliable genetic identification of burnt human remains. Forensic Sci Int Genet. doi:10.1016/j.fsigen.2010. 08.008.
19. Zołedziewska M, Gronkiewicz S, Dobosz T (2002) Comparison of various decalcificators in preparation of DNA from human rib bones. Anthropol Rev 65:75-80.