Characterization of the SpaCBA pilus fibers in the probiotic Lactobacillus rhamnosus GG

Applied and Environmental Microbiology

Volumn 78, Issue 7, 2012, Pages 2337-2344

  Reunanen, Justus ^a   von Ossowski, Ingemar ^a   Hendrickx, Antoni P A ^b,d   Palva, Airi ^a   de Vosa, Willem M ^a,c  

^a UNIVERSITY OF HELSINKI   (Finland)

^b UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^c WAGENINGEN UNIVERSITY   (Netherlands)

^d UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN; GENE CLUSTERS; GENOME SEQUENCES; GRAM-POSITIVE PATHOGENS; HOST TISSUES; LACTOBACILLUS RHAMNOSUS; PILUS FIBER; PROBIOTIC LACTOBACILLI; PROBIOTICS; THIN SECTION; UNIFORM DISTRIBUTION; WESTERN BLOTTING;

BODY FLUIDS; ELECTRON MICROSCOPY; GENES; TISSUE;

BACILLI;

FIMBRIA PROTEIN; PROBIOTIC AGENT;

BACTERIUM; CELL ORGANELLE; CLUSTER ANALYSIS; ELECTRON MICROSCOPY; GENE EXPRESSION; GENOME; PATHOGEN;

ARTICLE; BACTERIUM ADHERENCE; CHEMISTRY; ELECTRON MICROSCOPY; FIMBRIA; GENETICS; HUMAN; IMMUNOHISTOCHEMISTRY; LACTOBACILLUS RHAMNOSUS; METABOLISM; MUCUS; MULTIGENE FAMILY; PHYSIOLOGY; PROTEIN SUBUNIT; ULTRASTRUCTURE; WESTERN BLOTTING;

BACTERIAL ADHESION; BLOTTING, WESTERN; FIMBRIAE PROTEINS; FIMBRIAE, BACTERIAL; HUMANS; IMMUNOHISTOCHEMISTRY; LACTOBACILLUS RHAMNOSUS; MICROSCOPY, ELECTRON; MUCUS; MULTIGENE FAMILY; PROBIOTICS; PROTEIN SUBUNITS;

LACTOBACILLUS RHAMNOSUS; POSIBACTERIA;

EID: 84861113332     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.07047-11     Document Type: Article

References (31)

1. Bagnoli F, et al. 2008. A second pilus type in Streptococcus pneumoniae is prevalent in emerging serotypes and mediates adhesion to host cells. J. Bacteriol. 190:5480-5492.
2. Barocchi MA, et al. 2006. A pneumococcal pilus influences virulence and host inflammatory responses. Proc. Natl. Acad. Sci. U. S. A. 103:2857-2862.
3. Budzik JM, Marraffini LA, Schneewind O. 2007. Assembly of pili on the surface of Bacillus cereus vegetative cells. Mol. Microbiol. 66:495-510.
4. Budzik JM, et al. 2009. Intramolecular amide bonds stabilize pili on the surface of bacilli. Proc. Natl. Acad. Sci. U. S. A. 106:19992-19997.
5. Gaspar AH, Ton-That H. 2006. Assembly of distinct pilus structures on the surface of Corynebacterium diphtheriae. J. Bacteriol. 188:1526-1533.
6. Guttilla IK, et al. 2009. Acyl enzyme intermediates in sortase-catalyzed pilus morphogenesis in gram-positive bacteria. J. Bacteriol. 191:5603-5612.
7. Hendrickx AP, et al. 2008. Expression of two distinct types of pili by a hospital-acquired Enterococcus faecium isolate. Microbiology 154:3212-3223.
8. Hendrickx AP, Budzik JM, Oh SY, Schneewind O. 2011. Architects at the bacterial surface-sortases and the assembly of pili with isopeptide bonds. Nat. Rev. Microbiol. 9:166-176.
9. Hendrickx AP, Willems RJ, Bonten MJ, van Schaik W. 2009. LPxTG surface proteins of enterococci. Trends Microbiol. 17:423-430.
10. Kang HJ, Coulibaly F, Clow F, Proft T, Baker EN. 2007. Stabilizing isopeptide bonds revealed in gram-positive bacterial pilus structure. Science 318:1625-1628.
11. Kang HJ, Paterson NG, Gaspar AH, Ton-That H, Baker EN. 2009. The Corynebacterium diphtheriae shaft pilin SpaA is built of tandem Ig-like modules with stabilizing isopeptide and disulfide bonds. Proc. Natl. Acad. Sci. U. S. A. 106:16967-16971.
12. Kankainen M, et al. 2009. Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human-mucus binding protein. Proc. Natl. Acad. Sci. U. S. A. 106:17193-17198.
13. Kline KA, Falker S, Dahlberg S, Normark S, Henriques-Normark B. 2009. Bacterial adhesins in host-microbe interactions. Cell Host Microbe 5:580-592.
14. Lauer P, et al. 2005. Genome analysis reveals pili in group B Streptococcus. Science 309:105.
15. Mandlik A, Das A, Ton-That H. 2008. The molecular switch that activates the cell wall anchoring step of pilus assembly in gram-positive bacteria. Proc. Natl. Acad. Sci. U. S. A. 105:14147-14152.
16. Mandlik A, Swierczynski A, Das A, Ton-That H. 2008. Pili in Grampositive bacteria: assembly, involvement in colonization and biofilm development. Trends Microbiol. 16:33-40.
17. Mishra A, Das A, Cisar JO, Ton-That H. 2007. Sortase-catalyzed assembly of distinct heteromeric fimbriae in Actinomyces naeslundii. J. Bacteriol. 189:3156-3165.
18. Mora M, et al. 2005. Group A Streptococcus produce pilus-like structures containing protective antigens and Lancefield T antigens. Proc. Natl. Acad. Sci. U. S. A. 102:15641-15646.
19. Nallapareddy SR, et al. 2006. Endocarditis and biofilm-associated pili of Enterococcus faecalis. J. Clin. Investig. 116:2799-2807.
20. O'Connell Motherway M, et al. 2011. Functional genome analysis of Bifidobacterium breve UCC2003 reveals type IVb tight adherence (Tad) pili as an essential and conserved host-colonization factor. Proc. Natl. Acad. Sci. U. S. A. 108:11217-11222.
21. Proft T, Baker EN. 2009. Pili in Gram-negative and Gram-positive bacteria-structure, assembly and their role in disease. Cell. Mol. Life Sci. 66:613-635.
22. Quigley BR, Zahner D, Hatkoff M, Thanassi DG, Scott JR. 2009. Linkage of T3 and Cpa pilins in the Streptococcus pyogenes M3 pilus. Mol. Microbiol. 72:1379-1394.
23. Rosini R, et al. 2006. Identification of novel genomic islands coding for antigenic pilus-like structures in Streptococcus agalactiae. Mol. Microbiol. 61:126-141.
24. Scott JR, Zahner D. 2006. Pili with strong attachments: Gram-positive bacteria do it differently. Mol. Microbiol. 62:320-330.
25. Swaminathan A, et al. 2007. Housekeeping sortase facilitates the cell wall anchoring of pilus polymers in Corynebacterium diphtheriae. Mol. Microbiol. 66:961-974.
26. Swierczynski A, Ton-That H. 2006. Type III pilus of corynebacteria: pilus length is determined by the level of its major pilin subunit. J. Bacteriol. 188:6318-6325.
27. Telford JL, Barocchi MA, Margarit I, Rappuoli R, Grandi G. 2006. Pili in gram-positive pathogens. Nat. Rev. Microbiol. 4:509-519.
28. Ton-That H, Schneewind O. 2003. Assembly of pili on the surface of Corynebacterium diphtheriae. Mol. Microbiol. 50:1429-1438.
29. Ton-That H, Schneewind O. 2004. Assembly of pili in Gram-positive bacteria. Trends Microbiol. 12:228-234.
30. von Ossowski I, et al. 2010. Mucosal adhesion properties of the probiotic Lactobacillus rhamnosus GG SpaCBA and SpaFED pilin subunits. Appl. Environ. Microbiol. 76:2049-2057.
31. Yeung MK, Donkersloot JA, Cisar JO, Ragsdale PA. 1998. Identification of a gene involved in assembly of Actinomyces naeslundii T14V type 2 fimbriae. Infect. Immun. 66:1482-1491.