High-throughput screening of cohesin mutant libraries on cellulose microarrays

Methods in Enzymology

Volumn 510, Issue , 2012, Pages 453-463

  Slutzki, Michal ^a   Ruimy, Vered ^a   Morag, Ely ^b   Barak, Yoav ^a   Haimovitz, Rachel ^a   Lamed, Raphael ^c   Bayer, Edward A ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b Designer Energy   (Israel)

^c TEL AVIV UNIVERSITY   (Israel)

Author keywords

CBM; Cellulosome; Cohesin dockerin interaction; ELISA; Protein microarray

Indexed keywords

CARBOHYDRATE BINDING PROTEIN; CARRIER PROTEINS AND BINDING PROTEINS; CELLULOSE; CELLULOSOME; COHESIN; DNA; DOCKERIN; MUTANT PROTEIN; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; DNA EXTRACTION; ENZYME SPECIFICITY; HIGH THROUGHPUT SCREENING; MICROARRAY ANALYSIS; MOLECULAR CLONING; MOLECULAR DYNAMICS; MUTAGENESIS; PEPTIDE LIBRARY; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

EID: 84861112226     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-415931-0.00024-0     Document Type: Chapter

References (16)

1. Y. Barak, T. Handelsman, D. Nakar, A. Mechaly, R. Lamed, Y. Shoham, and E.A. Bayer Matching fusion protein systems for affinity analysis of two interacting families of proteins: The cohesin-dockerin interaction J. Mol. Recogn. 18 2005 491 501
2. E.A. Bayer, and R. Lamed The cellulose paradox: Pollutant par excellence and/or a reclaimable natural resource? Biodegradation 3 1992 171 188
3. E.A. Bayer, E. Morag, and R. Lamed The cellulosome - A treasure-trove for biotechnology Trends Biotechnol. 12 1994 378 386
4. E.A. Bayer, P.M. Coutinho, and B. Henrissat Cellulosome-like sequences in Archaeoglobus fulgidus: An enigmatic vestige of cohesin and dockerin domains FEBS Lett. 463 1999 277 280
5. E.A. Bayer, J.-P. Belaich, Y. Shoham, and R. Lamed The cellulosomes: Multi-enzyme machines for degradation of plant cell wall polysaccharides Annu. Rev. Microbiol. 58 2004 521 554
6. E.A. Bayer, R. Lamed, B.A. White, and H.J. Flint From cellulosomes to cellulosomics Chem. Rec. 8 2008 364 377
7. S. Chitayat, J.J. Adams, H.S. Furness, E.A. Bayer, and S.P. Smith The solution structure of the C-terminal modular pair from Clostridium perfringens reveals a non-cellulosomal dockerin module J. Mol. Biol. 381 2008 1202 1212
8. S. Chitayat, K. Gregg, J.J. Adams, E. Ficko-Blean, E.A. Bayer, A.B. Boraston, and S.P. Smith Three-dimensional structure of a putative non-cellulosomal cohesin module from a Clostridium perfringens family 84 glycoside hydrolase J. Mol. Biol. 375 2008 20 28
9. B. Friguet, L. Djavadi-Ohaniance, and M.E. Goldberg Some monoclonal antibodies raised with a native protein bind preferentially to the denatured antigen Mol. Immunol. 21 1984 673 677
10. R. Georgescu, G. Bandara, and L. Sun Saturation mutagenesis F.H. Arnold, G. Georgiou, Directed Evolution Library Creation: Methods and Protocols 2003 Humana Press Inc. Totowa, NJ
11. R. Haimovitz, Y. Barak, E. Morag, M. Voronov-Goldman, Y. Shoham, R. Lamed, and E.A. Bayer Cohesin-dockerin microarray: Diverse specificities between two complementary families of interacting protein modules Proteomics 8 2008 968 979
12. E. Morag, A. Lapidot, D. Govorko, R. Lamed, M. Wilchek, E.A. Bayer, and Y. Shoham Expression, purification and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum Appl. Environ. Microbiol. 61 1995 1980 1986
13. K. Ofir, Y. Berdichevsky, I. Benhar, R. Azriel-Rosenfeld, R. Lamed, Y. Barak, E.A. Bayer, and E. Morag Versatile protein microarray based on carbohydrate-binding modules Proteomics 5 2005 1806 1814
14. K. Ohmiya, K. Sakka, T. Kimura, and K. Morimoto Application of microbial genes to recalcitrant biomass utilization and environmental conservation J. Biosci. Bioeng. 95 2003 549 561
15. A. Peer, S.P. Smith, E.A. Bayer, R. Lamed, and I. Borovok Non-cellulosomal cohesin and dockerin-like modules in the three domains of life FEMS Microbiol. Lett. 291 2009 1 16
16. Slutzki, M., Barak, Y., Reshef, D., Schueler-Furman, O., Lamed, R., and Bayer, E. A. (in press). Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions. Methods Enzymol. Chapter 22