Detection of Candida and Aspergillus species DNA using broad-range real-time PCR for fungal endophthalmitis

Graefe's Archive for Clinical and Experimental Ophthalmology

Volumn 250, Issue 3, 2012, Pages 391-398

  Sugita, Sunao ^a   Kamoi, Koju ^a   Ogawa, Manabu ^a   Watanabe, Ken ^a   Shimizu, Norio ^a   Mochizuki, Manabu ^a  

^a TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

Author keywords

Endophthalmitis; Fungal infection; Polymerase chain reaction

Indexed keywords

ANTIBIOTIC AGENT; CORTICOSTEROID; FLUCONAZOLE; FUNGAL DNA; RNA 18S; ANTIFUNGAL AGENT;

ADULT; AGED; AQUEOUS HUMOR; ARTICLE; ASPERGILLOSIS; ASPERGILLUS; CANDIDA; CANDIDIASIS; CLINICAL ARTICLE; CLINICAL TRIAL; CONTROLLED STUDY; DNA DETERMINATION; EYE INFLAMMATION; FEMALE; FUNGAL ENDOPHTHALMITIS; FUNGAL GENOME; GENE DOSAGE; HUMAN; IDIOPATHIC DISEASE; MALE; NONHUMAN; PRIORITY JOURNAL; PROSPECTIVE STUDY; QUANTITATIVE DIAGNOSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE; UVEITIS; VITREOUS BODY; CASE REPORT; ENDOPHTHALMITIS; FUNGAL EYE INFECTION; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MIDDLE AGED; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

AGED; AGED, 80 AND OVER; ANTIFUNGAL AGENTS; AQUEOUS HUMOR; ASPERGILLOSIS; ASPERGILLUS; CANDIDA; CANDIDIASIS; DNA, FUNGAL; ENDOPHTHALMITIS; EYE INFECTIONS, FUNGAL; FEMALE; HUMANS; MALE; MIDDLE AGED; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY; VITREOUS BODY;

EID: 84860992201     PISSN: 0721832X     EISSN: 1435702X     Source Type: Journal    
DOI: 10.1007/s00417-011-1819-1     Document Type: Article

References (21)

1. Edwards JE Jr, Foos RY, Montgomerie JZ, Guze LB (1974) Ocular manifestations of Candida septicemia: review of seventysix cases of hematogenous Candida endophthalmitis. Medicine 53: 47-75
2. Rao NA, Hidayat AA (2001) Endogenous mycotic endophthalmitis: variations in clinical and histopathologic changes in candidiasis compared with aspergillosis. Am J Ophthalmol 132: 244-251
3. Klotz SA, Penn CC, Negvesky GJ, Butrus SI (2000) Fungal and parasitic infection of the eye. Clin Microbiol Rev 13: 662-685
4. Walsh TJ, Anaissie EJ, Denning DW, Herbrecht R, Kontoyiannis DP, Marr KA, Morrison VA, Segal BH, Steinbach WJ, Stevens DA, van Burik JA, Wingard JR, Patterson TF, Infectious Diseases Society of America (2008) Treatment of aspergillosis; clinical practice guidelines of the Infectious Disease Society of America. Clin Infect Dis 46: 327-360
5. Kunimoto DY, Das T, Sharma S, Jalali S, Majji AB, Gopinathan U, Athmanathan S, Rao TN (1999) Microbiologic spectrum and susceptibility of isolates: part I. Postoperative endophthalmitis. Endophthalmitis Research Group. Am J Ophthalmol 128: 240-242
6. Puliafito CA, Baker AS, Haaf J, Foster CS (1982) Infectious endophthalmitis. Review of 36 cases. Ophthalmology 89: 921-929
7. Lohmann CP, Linde HJ, Reischl U (2000) Improved detection of microorganisms by polymerase chain reaction in delayed endophthalmitis after cataract surgery. Ophthalmology 107: 1047-1051
8. Ferrer C, Colom F, Frasés S, Mulet E, Abad JL, Alió JL (2001) Detection and identification of fungal pathogens by PCR and by ITS2 and 5.8S ribosomal DNA typing in ocular infections. J Clin Microbiol 39: 2873-2879
9. Anand AR, Madhavan HN, Sudha NV, Therese KL (2001) Polymerase chain reaction in the diagnosis of Aspergillus endophthalmitis. Indian J Med Res 114: 133-140
10. Jaeger EE, Carroll NM, Choudhury S, Dunlop AA, Towler HM, Matheson MM, Adamson P, Okhravi N, Lightman S (2000) Rapid detection and identification of Candida, Aspergillus, and Fusarium species in ocular samples using nested PCR. J Clin Microbiol 38: 2902-2908
11. Sugita S, Shimizu N, Watanabe K, Katayama M, Horie S, Ogawa M, Takase H, Sugamoto Y, Mochizuki M (2011) Diagnosis of bacterial endophthalmitis by broad-range quantitative polymerase chain reaction. Br J Ophthalmol 95: 345-349
12. Kami M, Fukui T, Ogawa S, Kazuyama Y, Machida U, Tanaka Y, Kanda Y, Kashima T, Yamazaki Y, Hamaki T, Mori S, Akiyama H, Mutou Y, Sakamaki H, Osumi K, Kimura S, Hirai H (2001) Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis. Clin Infect Dis 33: 1504-1512
13. Akler ME, Vellend H, McNeely DM, Walmsley SL, Gold WL (1995) Use of fluconazole in the treatment of Candida endophthalmitis. Clin Infect Dis 20: 657-664
14. Ito M, Yokoi T, Sugita S, Shinohara N, Nishina S, Azuma N (2010) Endogenous Candida chorioretinitis in a healthy infant. Jpn J Ophthalmol 54: 629-631
15. Tarai B, Gupta A, Ray P, Shivaprakash MR, Chakrabarti A (2006) Polymerase chain reaction for early diagnosis of post-operative fungal endophthalmitis. Indian J Med Res 123: 671-678
16. Hidalgo JA, Alangaden GJ, Eliott D, Akins RA, Puklin J, Abrams G, Vazquez JA (2000) Fungal endophthalmitis diagnosis by detection of Candida albicans DNA in intraocular fluid by use of a species-specific polymerase chain reaction assay. J Infect Dis 181: 1198-1201
17. Shen X, Xu G (2009) Vitrectomy for endogenous fungal endophthalmitis. Ocul Immunol Inflamm 17: 148-152
18. Moinfar N, Smiddy WE, Miller D, Miller D, Herschel K (2007) Posttraumatic Aspergillus terreus endophthalmitis masquerading as dispersed lens fragments. J Cataract Refract Surg 33: 739-740
19. Kramer M, Kramer MR, Blau H, Bishara J, Axer-Siegel R, Weinberger D (2006) Intravitreal voriconazole for the treatment of endogenous Aspergillus endophthalmitis. Ophthalmology 113: 1184-1186
20. Kalina PH, Campbell RJ (1991) Aspergillus terreus endophthalmitis in a patient with chronic lymphocytic leukemia. Arch Ophthalmol 109: 102-103
21. Vollmer T, Störmer M, Kleesiek K, Dreier J (2008) Evaluation of novel broad-range real-time PCR assay for rapid detection of human pathogenic fungi in various clinical specimens. J Clin Microbiol 46: 1919-1926