Evaluation of the MALDI TOF-MS method for identification of Candida strains isolated from blood cultures

Diagnostic Microbiology and Infectious Disease

Volumn 73, Issue 1, 2012, Pages 65-67

  Yaman, Görkem ^a   Akyar, Işin ^a   Can, Simge ^b  

^a ACIBADEM UNIVERSITY   (Turkey)

^b Acibadem Labmed   (Turkey)

Author keywords

Blood cultures; Candida; ITS region sequence; MALDI TOF MS; VITEK 2

Indexed keywords

FUNGAL DNA; INTERNAL TRANSCRIBED SPACER;

ARTICLE; BLOOD CULTURE; CANDIDA; CANDIDA ALBICANS; CANDIDA DUBLINIENSIS; CANDIDA GLABRATA; CANDIDA KRUSEI; CANDIDA LAMBICA; CANDIDA PARAPSILOSIS; CANDIDA TROPICALIS; CLAVISPORA LUSITANIAE; CONTROLLED STUDY; DNA SEQUENCE; FUNGAL STRAIN; FUNGUS IDENTIFICATION; FUNGUS ISOLATION; INTERMETHOD COMPARISON; KLUYVEROMYCES MARXIANUS; LABORATORY AUTOMATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SPECIES IDENTIFICATION; TEST RETEST RELIABILITY;

BLOOD; CANDIDA; CANDIDEMIA; HUMANS; MICROBIOLOGICAL TECHNIQUES; MYCOLOGY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS;

EID: 84860762971     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2012.01.013     Document Type: Article

References (17)

1. Asadzadeh M., Ahmad S., Al-Sweih N., Khan Z.U. Rapid molecular differentiation and genotypic heterogeneity among Candida parapsilosis and Candida orthopsilosis strains isolated from clinical specimens in Kuwait. J. Med. Microbiol. 2009, 58:745-752.
2. Cuenca-Estrella M., Bernal-Martinez L., Buitrago M.J., Castelli M.V., Gomez-Lopez A., Zaragoza O., et al. Update on the epidemiology and diagnosis of invasive fungal infection. Int. J. Antimicrob. Agents 2008, 32:143-147.
3. Dhiman N., Hall L., Wohlfiel S.L., Buckwalter S.P., Wengenack N.L. Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast. J. Clin. Microbiol. 2011, 49:1614-1616.
4. Freydiere A.M., Guinet R., Boiron P. Yeast identification in the clinical microbiology laboratory: phenotypical methods. Med Mycol. 2001, 39:9-33.
5. Marinach-Patrice C., Fekkar A., Atanasova R., Gomes J., Djamdjian L., Brossas J.Y., et al. Rapid species diagnosis for invasive candidiasis using mass spectrometry. PLoS One 2010, e8862:5.
6. Marklein G., Josten M., Klanke U., Müller E., Horre R., Maier T., et al. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates. J. Clin. Microbiol. 2009, 47:2912-2917.
7. Morrell M., Fraser V.J., Kollef M.H. Delaying the empiric treatment of Candida bloodstream infection until positive blood culture results are obtained: a potential risk factor for hospital mortality. Antimicrob. Agents Chemother. 2005, 49:3640-3645.
8. Murali S., Langston A. Advances in antifungal prophylaxis and empiric therapy in patients with hematologic malignancies. Transpl Infect Dis 2009, 11:480-490.
9. Pappas P.G., Kauffman C.A., Andes D., Benjamin D.K., Calandra T.F., Edwards J.E., et al. Clinical practice guidelines for the management of candidiasis: 2009 update by the Infectious Diseases Society of America. Clin. Infect. Dis. 2009, 48:503-535.
10. Posteraro B., Torelli R., Carolis E.D., Posteraro P., Sanguinetti M. Update on the laboratory diagnosis of invasive fungal infections. Mediterr J Hematol Infect Dis 2011, 3:e2011002.
11. Reimer L.G., Wilson M.L., Weinstein M.P. Update on detection of bacteremia and fungemia. Clin. Microbiol. Rev. 1997, 10:444-465.
12. Sanguinetti M., Porta R., Sali M., La Sorda M., Pecorini G., Fadda G., et al. Evaluation of VITEK 2 and RapID yeast plus systems for yeast species identification: experience at a large clinical microbiology laboratory. J. Clin. Microbiol. 2007, 45:1343-1346.
13. Seyfarth F., Wiegand C., Erhard M., Graser Y., Elsner P., Hipler U.C. Identification of yeast isolated from dermatological patients by MALDI-TOF mass spectrometry. Mycoses 2012, 55:276-280.
14. Stevenson L.G., Drake S.K., Shea Y.R., Zelazny A.M., Murray P.R. Evaluation of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF) for the identification of clinically important yeast species. J. Clin. Microbiol. 2010, 48:3482-3486.
15. van Veen S.Q., Claas E.C.J., Kuijper E.J. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) in routine medical microbiology laboratory. J. Clin. Microbiol. 2010, 48:900-907.
16. Verweij P.E., Breuker I.M., Rijs A., Meis J. Comparative study of seven commercial yeast identification systems. J Clin Pathol 1999, 52:271-273.
17. White T., Bruns T., Lee S., Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 1990, Academic Press, San Diego, CA. M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White (Eds.).