Metabolite profiling reveals clear metabolic changes during somatic embryo development of Norway spruce (Picea abies)

Tree Physiology

Volumn 32, Issue 2, 2012, Pages 232-244

  Businge, Edward ^a   Brackmann, Klaus ^a,b   Moritz, Thomas ^a   Egertsdotter, Ulrika ^a,c  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^b GREGOR MENDEL INSTITUTE OF MOLECULAR PLANT BIOLOGY   (Austria)

^c GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Gas chromatography mass spectrometry; Metabolite profiling; Metabolomics; Norway spruce (Picea abies); Somatic embryogenesis

Indexed keywords

CONIFEROUS TREE; DEVELOPMENTAL STAGE; EMBRYONIC DEVELOPMENT; HORMONE; METABOLISM; METABOLITE; PLANT BREEDING; SUGAR;

ARTICLE; CELL LINE; GROWTH, DEVELOPMENT AND AGING; MASS FRAGMENTOGRAPHY; METABOLISM; METABOLOMICS; MULTIVARIATE ANALYSIS; PHYSIOLOGICAL STRESS; PLANT SEED; PRENATAL DEVELOPMENT; SPRUCE;

CELL LINE; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; METABOLOMICS; MULTIVARIATE ANALYSIS; PICEA; SEEDS; STRESS, PHYSIOLOGICAL;

CONIFEROPHYTA; PICEA ABIES;

EID: 84860618860     PISSN: 0829318X     EISSN: 17584469     Source Type: Journal    
DOI: 10.1093/treephys/tpr142     Document Type: Article

References (63)

1. Astarita, L.V., E.I.S. Floh and W. Handro. 2003. Changes in IAA, tryptophan and activity of soluble peroxidase associated with seed development in Araucaria angustifolia. Biol. Plant. 47:53-59.
2. Aubert, S., E. Gout, R. Blingny and R. Douce. 1994. Multiple effects of glycerol on plant metabolism. Phosphorus-31 nuclear magnetic resonance studies. J. Biol. Chem. 269:21420-21427.
3. Ball, E. 1953. Hydrolysis of sucrose by autoclaving media, a neglected aspect in the culture of plant tissues. Bull. Torrey Bot. Club 80:409-411.
4. Basciano, H., L. Federico and K. Adeli. 2005. Fructose, insulin resistance, and metabolic dyslipidemia. Nutr. Metab. 2:5. doi:10.1186/1743-7075- 2-5. (Pubitemid 40412656)
5. Bozhkov, P.V. and S. von Arnold. 1998. Polyethylene glycol promotes maturation but inhibits further development of Picea abies somatic embryos. Physiol. Plant. 104:211-224. (Pubitemid 28547974)
6. Bozhkov, P.V., L.H. Filonova and M.F. Suarez. 2005. Programmed cell death in plant embryogenesis. Curr. Top. Dev. Biol. 67:135-179. (Pubitemid 43588851)
7. Broeckling, C.D., D.V. Huhman, M.A. Farag, J.T. Smith, G.D. May, P. Mendes, R.A. Dixon and L.W. Sumner. 2005. Metabolic profiling of Medicago truncatula cell cultures reveals the effects of biotic and abiotic elicitors on metabolism. J. Exp. Bot. 56:323-336. (Pubitemid 40181133)
8. Bylesjö, M., M. Rantalainen, O. Cloarec, J.K. Nicholson, E. Holmes and J. Trygg. 2006. OPLS discriminant analysis: combining the strengths of PLS-DA and SIMCA classification. J. Chemometr. 20:341-351.
9. Canovas, M.F., A. Concepcion, F.R. Canton, R.A. Canas and F. de la Torre. 2007. Ammonium assimilation and amino acid metabolism in conifers. J. Exp. Bot. 58:2307-2318. (Pubitemid 47216542)
10. Cho, Y-H. and S.-D. Yoo. 2011. Signaling role of fructose mediated by FINS1/FBP in Arabidopsis thaliana. PLoS Genet. 7:e1001263. doi:10.1371/journal. pgen.1001263.
11. Clapham, D. and U. Egertsdotter. 2011. Method for maturing and synchronizing somatic embryos. International application number PCT/ SE2011/050356, October 6.
12. Cooke, T.J., R.H. Racusen and J.D. Cohen. 1993. The role of auxin in plant embryogenesis. Plant Cell 5:1494-1495.
13. Dettmer, K., P.A. Aranov and B.D. Hammock. 2007. Mass spectrometry- based metabolomics. Mass Spectro. Rev. 26:51-78.
14. Dowlatabadi, R., A.M. Weljie, T.A. Thorpe, E.C. Yeung and H.J. Vogel. 2009. Metabolic foot printing study of white spruce somatic embryogenesis using NMR spectroscopy. Plant Physiol. Biochem. 47:343-350.
15. Egertsdotter, U. and S. von Arnold. 1995. Importance of arabinogalactan proteins for the development of somatic embryos of Norway spruce (Picea abies). Physiol. Plant. 93:34-45.
16. Egertsdotter, U., L.H. Mo and S. von Arnold. 1993. Extracellular proteins in embryogenic suspension cultures of Norway spruce (Picea abies). Physiol. Plant. 88:315-21.
17. Filonova, L.H., P.V. Bozhkov and S. von Arnold. 2003. Developmental pathway of somatic embryogenesis in Picea abies as revealed by time-lapse tracking. J. Exp. Bot. 51:249-264. (Pubitemid 30097613)
18. Gosslová, M., H. Svobodova, H. Lipavská, J. Albrechtová and D. Vreugdenhil. 2001. Comparing carbohydrate status during Norway spruce seed development and somatic embryo formation. In Vitro Cell. Dev. Biol. Plant 37:24-28. (Pubitemid 32956238)
19. Gullberg, J., P. Jonsson, A. Nordstrom, M. Sjostrom and T. Moritz. 2004. Design of experiments: an efficient strategy to identify factors influencing extraction and derivatization of Arabidopsis thaliana samples in metabolomic studies with gas chromatography/mass spectrometry. Anal. Biochem. 331:283-295. (Pubitemid 38953079)
20. Hakman, I., H. Hallberg and J. Palovaara. 2009. The polar auxin transport inhibitor NPA impairs morphology and increases the expression of an auxin efflux facilitator protein PIN during Picea abies somatic embryo development. Tree Physiol. 29:483-496.
21. Handa, S., R.A. Bressan, A.K. Handa and N.C. Carpita. 1983. Solutes contributing to osmotic adjustment in cultured plant cells adapted to water stress. Plant Physiol. 73:834-843.
22. Helmersson, A., S. von Arnold, K. Burg and P. Bozhkov. 2004. High stability of nuclear microsatellite loci during early stages of somatic embryogenesis in Norway spruce. Tree Physiol. 24:1181-1186. (Pubitemid 39533646)
23. Hosp, J., A. Tashpulatov, U. Roessner, et al. 2007. Transcriptional and metabolic profiles of stress-induced embryogenic tobacco microspores. Plant Mol. Biol. 63:137-149. (Pubitemid 44950238)
24. Jiménez, V.M. 2001. Regulation of in vitro somatic embryogenesis with emphasis on the role of endogenous hormones. Rev. Bras. Fisiol. Veg. 13:196-223. (Pubitemid 34001825)
25. Jonsson, P., A.I. Johansson, J. Gullberg, J.A.J. Trygg, B. Grung, S. Marklund, M. Sjostrom, H. Antii and T. Moritz. 2005. High throughput data analysis for detecting and identifying differences between samples in GC/MS-based metabolomic analyses. Anal. Chem. 77:5635-5642. (Pubitemid 41268639)
26. Kato-Naguchi, H., T. Takaoka and K. Izumori. 2005. Psicose inhibits lettuce root growth via a hexokinase independent pathway. Physiol. Plant. 125:293-298. (Pubitemid 41565104)
27. Kermode, A.R. 1990. Regulatory mechanisms involved in the transition from seed development to germination. Crit. Rev. Plant Sci. 9:155-194.
28. Kong, L., S.M. Attree and L.C. Fowke. 1997. Changes of endogenous hormone levels in developing seeds, zygotic embryos and megagametophytes in Picea glauca. Physiol. Plant. 101:23-30. (Pubitemid 28286088)
29. Larsson, E., F. Sitbon, K. Ljung and S. von Arnold. 2008. Inhibited polar auxin transport results in aberrant embryo development in Norway spruce. New Phytol. 177:356-366. (Pubitemid 350295786)
30. Lipavská, H. and H. Konrádová. 2004. Somatic embryogenesis in conifers: the role of carbohydrate metabolism. In Vitro Cell. Dev. Biol. Plant 40:23-30. (Pubitemid 38305850)
31. Lipavská, H., H. Svobodová, J. Albrechtová, L. Kumstýøová, M. Vágner and Z. Vondrá ková. 2000. Carbohydrate status during somatic embryo maturation in Norway spruce. In Vitro Cell Dev. Biol. Plant 36:260-267.
32. Lipert, D., J. Zhuang, S. Ralph, D.E. Ellis, M. Gilbert, R. Olafson, K. Ritland, C.J. Douglas and J. Bohlmann. 2005. Proteome analysis of early somatic embryogenesis in Picea glauca. Proteomics 5:461-473. (Pubitemid 40262064)
33. Madsen, R., T. Lundstedt and J. Trygg. 2010. Chemometrics in metabolomics - a review in human disease diagnosis. Anal. Chim. Acta 659:23-33.
34. Michalczuk, L., T.J. Cooke and J.D. Cohen. 1992. Auxin levels at different stages of carrot embryogenesis. Phytochemistry 31:1097-1103.
35. Minocha, R., S.C. Minocha and S. Long. 2004. Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.). In Vitro Cell. Dev. Biol. Plant 40:572-580.
36. Mo, L.H., U. Egertsdotter and S. von Arnold. 1996. Secretion of specific extracellular proteins by somatic embryos of Picea abies is dependent on embryo morphology. Ann. Bot. 77:143-152.
37. Norgaard, J.V. 1997. Somatic embryo maturation and plant regeneration in Abies nordmanniana Lk. Plant Sci. 124:211-221. (Pubitemid 27224766)
38. Pullman, G.S. and M. Buchanan. 2008. Identification and quantitative analysis of stage-specific carbohydrates in loblolly pine (Pinus taeda) zygotic embryo and female gametophyte tissues. Tree Physiol. 28:985-996.
39. Pullman, G.S., S. Johnson, G. Peter, J. Cairney and N. Xu. 2003. Improving loblolly pine somatic embryo maturation: comparison of somatic and zygotic embryo morphology, germination, and gene expression. Plant Cell Rep. 21:747-758. (Pubitemid 36590332)
40. Robinson, A.R., K. Nicholas, N.K. Ukrainetz, K.-Y. Kang and S.D. Mansfield. 2007. Metabolite profiling of Douglas-fir (Pseudotsuga menziesii) field trials reveals strong environmental and weak genetic variation. New Phytol. 174:762-773. (Pubitemid 46743888)
41. Robinson, A.R., R. Dauwe, N.K. Ukrainetz, I.F. Cullis, R. White and S.D. Mansfield. 2009. Predicting the regenerative capacity of conifer somatic embryogenic cultures by metabolomics. Plant Biotechnol. J. 7:952-963.
42. Rose, R.J., F.R. Mantiri, S. Kurdyukov, S.-K. Chen, X.-D. Wang, K.E. Nolan and M.B. Sheahan. 2010. Developmental biology of somatic embryogenesis. In Plant Developmental Biology - Biotechnological Perspectives. Eds. E.C. Pua and M.R. Davey. Springer, Berlin, pp 3-21.
43. Schauer, N., D. Steinhauser, S. Strelkov, et al. 2005. GC-MS libraries for the rapid indentification of metabolites in complex biological samples. FEBS Lett. 579:1332-1337. (Pubitemid 40289176)
44. Schuller, A. and G. Reuther. 1993. Response of Abies alba embryonalsuspensor mass to various carbohydrate treatments. Plant Cell Rep. 12:199-202.
45. Schiavone, F.M. and T.J. Cooke. 1987. Unusual patterns of somatic embryogenesis in the domesticated carrot: developmental effects of exogenous auxins and auxin transport inhibitors. Cell Differ. 21:53-62. (Pubitemid 17098256)
46. Shelp, B.J., A.W. Bown and M.D. McLean. 1999. Metabolism and functions of gamma-aminobutyric acid. Trends Plant Sci. 4:446-452. (Pubitemid 29506767)
47. Srivastava, V. 2009. Active oxygen involvement in developmental processes in Populus. PhD thesis, Swedish University of Agricultural Sciences, Umeå, Sweden.
48. Sitbon, F., C. Åstot, A. Edlund, A. Crozier and G. Sandberg. 2000. The relative importance of tryptophan-dependent and tryptophan-independent biosynthesis of indole-3-acetic acid in tobacco. Planta 211:715-721.
49. Sturm, A. 1999. Invertases. Primary structures, functions, and roles in plant development and sucrose partitioning. Plant Physiol. 121:1-8.
50. Sumner, L., P. Mendes and R. Dixon. 2003. Plant metabolomics: largescale phytochemistry in the functional genomics era. Phytochemistry 62:817-836. (Pubitemid 36262386)
51. Svobodova, H., J. Albrechtová, L. Kumstý?ová, H. Lipavská, M. Vágner and Z. Vondráková. 1999. Somatic embryogenesis in Norway spruce: anatomical study of embryo development and influence of polyethylene glycol on maturation process. Plant Physiol. Biochem. 37:209-221. (Pubitemid 29270528)
52. Thomas, P. and M.B. Ravindra. 1997. Shoot-tip culture in mango: influence of medium, genotype, explant factors, season and decontamination treatments on phenolic exudation, explant survival and axenic culture establishment. J. Hortic. Sci. Biotech. 72:713-722.
53. Tremblay, L. and F.M. Tremblay. 1991. Carbohydrate requirements for the development of black spruce (Picea mariana (Mill.) B.S.P) and red spruce (P. rubens Sarg.) somatic embryos. Plant Cell Tiss. Organ Cult. 27:95-103.
54. Trygg, J. and S. Wold. 2002. Orthogonal projections to latent structures (O-PLS). J. Chemometr. 16:119-128.
55. Vernon, D.M. and H.J. Bohnert. 1992. A novel methyl transferase induced by osmotic stress in the facultative halophyte Mesembryanthemum crystallium. EMBO J. 11:2077-2085.
56. Verslues, P.E. and R.E. Sharp. 1999. Proline accumulation in maize (Zea mays L.) primary roots at low water potentials. II. Metabolic source of increased proline deposition in elongation zone. Plant Physiol. 119:1349-1360. (Pubitemid 29388230)
57. Vestman, D., E. Larsson, J.C. Uddenberg, D. Clapham, E. Sundberg and S. von Arnold. 2011. Important processes during differentiation of somatic embryos of Norway spruce as revealed by changes in global gene expression. Tree Genet. Genomes 7:347-362.
58. von Arnold, S. and T. Eriksson. 1981. In vitro studies of adventitious shoot formation in Pinus contorta. Can. J. Bot. 59:870-874.
59. von Arnold, S., I. Sabala, P. Bozhkov, J. Dyachok and L. Filanova. 2002. Developmental pathways of somatic embryogenesis. Plant Cell Tiss. Organ Cult. 69:233-249. (Pubitemid 34674141)
60. Vondráková, Z., K. Eliášová, L. Fischerová and M. Vágner. 2011. The role of auxins in somatic embryogenesis of Abies alba. Cent. Eur. J. Biol. 4:587-596.
61. Weigelt, K., H. Kuster, R. Radchuk, M. Muller, H. Weichert, A. Fait, A.R. Fernie, I. Saalbach and H. Weber. 2008. Increasing amino acid supply in pea embryos reveals specific interactions of N and C metabolism, and highlights the importance of mitochondrial metabolism. Plant J. 55:909-926.
62. Wiklund, S., E. Johansson, L. Snowstorm, E.J. Mellerowicz, U. Edlund, J.P. Schockcor, J. Gottfries, T. Moritz and J. Trygg. 2008. Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models. Anal. Chem. 80:115-122. (Pubitemid 351448411)
63. Wold, S., K. Esbensen and P. Geladi. 1987. Principal component analysis. Chemometr. Intell. Lab. Syst. 2:37-52.