Transmission electron microscopy of thin sections of Drosophila: High-pressure freezing and freeze-substitution

Cold Spring Harbor Protocols

Volumn 7, Issue 4, 2012, Pages 510-515

  McDonald, Kent L ^a   Sharp, David J ^a   Rickoll, Wayne ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ORGANIC SOLVENT;

ANIMAL TISSUE; ARTICLE; CELL ULTRASTRUCTURE; CRYOPRESERVATION; DROSOPHILA; EMBRYO; FREEZING; HIGH PRESSURE FREEZING; LARVA; NONHUMAN; PROTEIN IMMOBILIZATION; TISSUE FIXATION; TRANSMISSION ELECTRON MICROSCOPY; ANIMAL; FREEZE DRYING; HYDROSTATIC PRESSURE; METHODOLOGY; MICROTOMY; ULTRASTRUCTURE;

ANIMALS; DROSOPHILA; FREEZE SUBSTITUTION; FREEZING; HYDROSTATIC PRESSURE; MICROSCOPY, ELECTRON, TRANSMISSION; MICROTOMY;

EID: 84860590094     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot068403     Document Type: Article

References (7)

1. Howard RJ, O'Donnell KL. 1987. Freeze substitution of fungi for cytological analysis. Exp Mycol 11: 250-269.
2. McDonald K. 1994. Electron microscopy and EM immunocytochemistry. Methods Cell Biol 44: 411-444.
3. McDonald K. 1999. High pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling. Methods Mol Biol 117: 77-97.
4. McDonald KL, Sharp DJ, Rickoll W. 2012. Transmission electron microscopy of thin sections of Drosophila: Conventional chemical fixation of embryos using trialdehyde. Cold Spring Harb Protoc doi: 10.1101/pdb.prot068411.
5. Moor H. 1987. Theory and practice of high pressure freezing. In Cryotechniques in biological electron microscopy (ed. Steinbrecht RA, Zierold K), pp. 175-191. Springer, Berlin.
6. Newman GR, Hobot JA. 1993. Resin microscopy and on-section immunocytochemistry. Springer, Berlin.
7. Steinbrecht RA, Müller M. 1987. Freeze substitution and freeze drying. In Cryotechniques in biological electron microscopy (ed. Steinbrecht RA, Zierold K), pp. 149-172. Springer, Berlin.