Measuring resting membrane potential using the fluorescent voltage reporters DiBAC4(3) and CC2-DMPE

Cold Spring Harbor Protocols

Volumn 7, Issue 4, 2012, Pages 459-464

  Adams, Dany S ^a   Levin, Michael ^a  

^a TUFTS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIS(1,3 DIETHYLTHIOBARBITURIC ACID)TRIMETHINE OXONOL; FLUORESCENT BIOELECTRIC REPORTER; FLUORESCENT DYE; N (6 CHLORO 7 HYDROXYCOUMARIN 3 CARBONYL)DIMYRISTOYLPHOSPHATIDYLETHANOLAMINE; UNCLASSIFIED DRUG; BARBITURIC ACID DERIVATIVE; BIS(1,3 DIBUTYLBARBITURIC ACID)TRIMETHINE OXONOL; BIS(1,3-DIBUTYLBARBITURATE)TRIMETHINE OXONOL; COUMARIN DERIVATIVE; ETHANOLAMINE DERIVATIVE; ISOXAZOLE DERIVATIVE; N (6 CHLORO 7 HYDROXYCOUMARIN 3 CARBONYL) DIMYRISTOYLPHOSPHATIDYL ETHANOLAMINE; N-(6-CHLORO-7-HYDROXYCOUMARIN-3-CARBONYL)-DIMYRISTOYLPHOSPHATIDYL-ETHANOLAMINE;

ACTION POTENTIAL; ANIMAL CELL; ARTICLE; CELL SELECTION; CONTROLLED STUDY; DEPOLARIZATION; ELECTROPHYSIOLOGY; EMBRYO; FLUORESCENCE MICROSCOPY; MEMBRANE STEADY POTENTIAL; NONHUMAN; TADPOLE; TIME LAPSE IMAGING; XENOPUS LAEVIS; ANIMAL; CELL CULTURE; CELL MEMBRANE POTENTIAL; CYTOLOGY; METABOLISM; METHODOLOGY; PRENATAL DEVELOPMENT; STAINING; ZEBRA FISH;

DANIO RERIO; XENOPUS LAEVIS;

ANIMALS; BARBITURATES; CELLS, CULTURED; COUMARINS; CYTOLOGICAL TECHNIQUES; ETHANOLAMINES; FLUORESCENT DYES; ISOXAZOLES; MEMBRANE POTENTIALS; MICROSCOPY, FLUORESCENCE; STAINING AND LABELING; XENOPUS LAEVIS; ZEBRAFISH;

EID: 84860567773     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot067702     Document Type: Article

References (4)

1. Adams DS, Levin M. 2012. General principles for measuring resting membrane potential and ion concentration using fluorescent bioelectricity reporters. Cold Spring Harb Protoc doi: 10.1101/pdb.top067710.
2. Kuznetsov A, Bindokas VP, Marks JD, Philipson LH. 2005. FRET-based voltage probes for confocal imaging: membrane potential oscillations throughout pancreatic islets. Am J Physiol Cell Physiol 289: C224-C229.
3. Maher MP, Wu N-T, Ao H. 2007. pH-Insensitive FRET voltage dyes. J Biomol Screen 12: 656-667.
4. Vandenberg LN, Morrie RD, Adams DS. 2011. V-ATPase-dependent ectodermal voltage and pH regionalization are required for craniofacial morphogenesis. Dev Dyn 240: 1889-1904.