Fluorescent labeling of myosin V for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays

Cold Spring Harbor Protocols

Volumn 7, Issue 5, 2012, Pages 620-622

  Beausang, John F ^a   Sun, Yujie ^a   Quinlan, Margot E ^a   Forkey, Joseph N ^a   Goldman, Yale E ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; CALCIUM; CALMODULIN; FLUORESCENT DYE; MYOSIN V; RHODAMINE;

ARTICLE; ASSAY; CHICK; FLUORESCENCE MICROSCOPY; MOTILITY ASSAY; NONHUMAN; POLARIZED TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY; PROTEIN PURIFICATION; STOICHIOMETRY; ANIMAL; CHEMISTRY; CHICKEN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOTION; STAINING;

ANIMALS; CHICKENS; FLUORESCENT DYES; MICROSCOPY, FLUORESCENCE; MOTION; MYOSIN TYPE V; RHODAMINES; STAINING AND LABELING;

EID: 84860556542     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot069369     Document Type: Article

References (5)

1. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012a. Fluorescent labeling of calmodulin with bifunctional rhodamine. Cold Spring Harb Protoc doi: 10.1101/pdb.prot069351.
2. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012b. Preparation of filamentous actin for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays. Cold Spring Harb Protoc doi: 10.1101/pdb.prot069377.
3. Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE. 2012c. Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy (polTIRFM). Cold Spring Harb Protoc doi: 10.1101/pdb.top069344.
4. Cheney RE. 1998. Purification and assay of myosin V. Methods Enzymol 298: 3-18.
5. Putkey JA, Slaughter GR, Means AR. 1985. Bacterial expression and characterization of proteins derived from the chicken calmodulin cDNA and a calmodulin processed gene. J Biol Chem 260: 4704-4712.