Novel approach for the generation of recombinant African swine fever virus from a field isolate using GFP expression and 5-bromo-2'-deoxyuridine selection

Journal of Virological Methods

Volumn 183, Issue 1, 2012, Pages 86-89

  Portugal, Raquel ^a   Martins, Carlos ^b   Keil, Günther M ^a  

^a FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

^b FACULDADE DE MEDICINA VETERINÁRIA   (Portugal)

Author keywords

African swine fever virus recombinants; BrdU selection; Field isolate; Green fluorescent protein; Thymidine kinase

Indexed keywords

BROXURIDINE; ENHANCED GREEN FLUORESCENT PROTEIN; THYMIDINE KINASE;

AFRICAN SWINE FEVER VIRUS; ANIMAL CELL; ARTICLE; AUTOFLUORESCENCE; CONTROLLED STUDY; LUNG ALVEOLUS CELL; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROGENY; PROTEIN EXPRESSION; VERO CELL; VIRUS GENOME; VIRUS ISOLATION; VIRUS RECOMBINANT; VIRUS REPLICATION; VIRUS STRAIN; WILD TYPE;

AFRICAN SWINE FEVER VIRUS; ANIMALS; ANTIVIRAL AGENTS; BROMODEOXYURIDINE; CELLS, CULTURED; GREEN FLUORESCENT PROTEINS; PLASMIDS; RECOMBINATION, GENETIC; SELECTION, GENETIC; STAINING AND LABELING; SUS SCROFA; VIROLOGY;

AFRICAN SWINE FEVER VIRUS; SUS SCROFA;

EID: 84860555334     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.03.030     Document Type: Article

References (17)

1. Bello L.J., Whitbeck J.C., Lawrence W.C. Map location of the thymidine kinase gene of bovine herpesvirus 1. Journal of Virology 1987, 61:4023-4025.
2. Brun A., Rodríguez F., Parra F., Sobrino F., Escribano J.M. Design and construction of African swine fever virus chimeras incorporating foreign viral epitopes. Archives of Virology 1999, 144:1287-1298.
3. Costard S., Wieland B., de Glanville W., Jori F., Rowlands R., Vosloo W., Roger F., Pfeiffer D.U., Dixon L.K. African swine fever: how can global spread be prevented?. Philosophical Transactions of the Royal Society of London B: Biological Sciences 2009, 364:2683-2696.
4. Dixon, L.K., Escribano, J.M., Martins, C., Rock, D.L., Salas, M.L., Wilkinson, P.J., 2005. In: Fauquet, C.M., Mayo, M.A., Maniloff, J, Desselberger, U., Ball, L.A. (Eds), Vírus Taxonomy, VIIIth Report of the ICTV, Elsevier/Academic Press, London, pp. 135-143.
5. Garcia-Escudero R., Andrés G., Almazán F., Viñuela E. Inducible gene expression from African swine fever virus recombinants: analysis of the major capsid protein p72. Journal of Virology 1998, 72:3185-3195.
6. García R., Almazán F., Rodríguez J.M., Alonso M., Viñuela E., Rodríguez J.F. Vectors for the geneic manipulation of African swine fever virus. Journal of Biotechnology 1995, 40:121-131.
7. Hurtado C., Bustos M.J., Carrascosa A.L. The use of COS-1 cells for studies of field and laboratory African swine fever virus samples. Journal of Virological Methods 2010, 164:131-134.
8. Kit S., Dubbs D.R., Frearson P.M. HeLa cells resistant to bromodeoxyuridine and deficient in thymidine kinase activity. International Journal of Cancer 1966, 1:19-30.
9. Leitao A., Cartaxeiro C., Coelho R., Cruz B., Parkhouse R.M.E., Portugal F.C., Vigario J.D., Martins C.L.V. The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response. Journal of General Virology 2001, 82:513-523.
10. López-Otín C., Freije J.M., Parra F., Méndez E., Viñuela E. Mapping and sequence of the gene coding for protein p72, the major capsid protein of African swine fever virus. Virology 1990, 175:477-484.
11. Moore D.M., Zsak L., Neilan J.G., Lu Z., Rock D.L. The African swine fever virus thymidine kinase gene is required for efficient replication in swine macrophages and for virulence in swine. Journal of Virology 1998, 72:10310-10315.
12. Rahimi P., Sohrabi A., Ashrafihelan J., Edalat R., Alamdari M., Masoudi M., Mostofi S., Azadmanesh K. Emergence of African swine fever virus northwestern Iran. Emerging Infectious Diseases 2010, 16:1946-1948.
13. Rowlands R.J., Michaud V., Heath L., Hutchings G., Oura C., Vosloo W., Dwarka R., Onashvili T., Albina E., Dixon L.K. African swine fever virus isolate, Georgia, 2007. Emerging Infectious Diseases 2008, 14:1870-1874.
14. Tulman E.R., Delhon G.A., Ku B.K., Rock D.L. African swine fever virus. Current Topics in Microbiology and Immunology 2009, 328:43-87.
15. Van Regenmortel M.H., Burke D.S., Calisher C.H., Dietzgen R.G., Fouquet C.M., Ghabrial S.A., Jahrling P.B., Johnson K.M., Holbrook M.R., Horzinek M.C., Keil G.M., Kuhn J.H., Mahy B.W., Martelli G.P., Pringle C., Rybicki E.P., Skern T., Tesh R.B., Wahl-Jensen V., Walker P.J., Weaver S.C. A proposal to change existing virus species names to non-Latinized binomials. Archives of Virology 2010, 155:1909-1919.
16. Vigário J.D., Terrinha A.M., Moura Nunes J.F. Antigenic relationships among strains of African swine fever virus. Archiv fur Die Gesamte Virusforschung 1974, 45:272-277.
17. Yáñez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Viñuela E. Analysis of the complete nucleotide sequence of African swine fever virus. Virology 1995, 208:249-278.