Mycobacterium paratuberculosis shedding into milk: Association of ELISA seroreactivity with DNA detection in milk

International Journal of Applied Research in Veterinary Medicine

Volumn 6, Issue 2, 2008, Pages 137-144

  Pinedo, Pablo J ^a   Williams, Joseph E ^a   Monif, Gilles R G ^b   Rae, D Owen ^a   Buergelt, Claus D ^a  

^a University of Florida   (United States)

^b Infectious Diseases Incorporated   (United States)

Author keywords

ELISA; Milk; Paratuberculosis; PCR

Indexed keywords

EID: 84860539899     PISSN: 15422666     EISSN: 1559470X     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Whitlock RH, Buergelt CD: Preclinical and clini-cal manifestations of paratuberculosis (including pathology). Vet Clin N Am 1996;12:357-371.
2. Office International Des Epizooties (OIE): World Animal Health, 2004 Part II.
3. Taylor TK, Wilks CR, McQueen DS: Isolation of Mycobacterium paratuberculosis from the milk of a cow with Johne's disease. Vet Rec 1981;109:532-533.
4. Giese SB, Ahrens P: Detection of Mycobacte-rium avium subsp. paratuberculosis in milk from clinically affected cows by PCR and culture. Vet Microbiol 2000;77:291-297.
5. Streeter RN, Hoffsis GF, Bech-Nielsen S, Shulaw WP, Rings DM: Isolation of Mycobacterium para-tuberculosis from colostrum and milk of subclini-cally infected cows. Am J Vet Res 1995;56:1322-1324.
6. Grant IR, Williams AG, Rowe MT, Muir DD: Ef-ficacy of various pasteurization time-temperature conditions in combination with homogenization on inactivation of Mycobacterium avium subsp. paratuberculosis in milk. Appl Environ Microbiol 2005;71:2853-2861.
7. Naser SA, Ghobrial G, Romero C, Valentine JF: Culture of Mycobacterium avium subsp. paratu-berculosis from the blood of patients with Crohn's disease. Lancet 2004;364:1039-1044.
8. Millar D, Ford J, Sanderson J, et al: IS900 PCR to detect Mycobacterium paratuberculosis in retail supplies of whole pasteurized cows' milk in England and Wales. Appl Environ Microbiol 1996;62:3446-3452.
9. Ayele WY, Svastova P, Roubal P, Bartos M, Pavlik I: Mycobacterium avium subspecies paratuber-culosis cultured from locally & commercially pasteurized cow's milk in the Czech Republic. Appl Environ Microbiol 2005;71:1210-1214.
10. Ellingson JL, Anderson JL, Koziczkowski JJ, et al: Detection of viable Mycobacterium avium subsp. paratuberculosis in retail pasteurized whole milk by two culture methods and PCR. J Food Prot 2005;68:966-972.
11. Whitlock RH, Wells SJ, Sweeney RW, Van Tiem J: ELISA and fecal culture for paratuberculosis (Johne's disease): sensitivity and specificity of each method. Vet Microbiol 2000;77:387-398.
12. Buergelt CD, Williams JE: Nested PCR on blood and milk for the detection of Mycobacterium avium subsp paratuberculosis DNA in clinical and subclinical bovine paratuberculosis. Aust Vet J 2004;82:497-503.
13. Vary PH, Andersen PR, Green E, Hermon-Taylor J, Mcfadden JJ. Use of highly specific DNA probes and the polymerase chain reaction to detect Myco-bacterium paratuberculosis in Johne's disease. J Clin Microbiol 1990;28:933-937.
14. Braun RK, Buergelt CD, Little RC, Linda SB, Simpson JR. Use of an enzyme-linked immuno-sorbent assay to estimate prevalence of paratuber-culosis in cattle of Florida. J Am Vet Med Assoc 1990;196:1251-1254.
15. Bech-Nielsen S, Jorgensen JB, Ahrens P, Feld NC: Diagnostic accuracy of Mycobacterium phlei-absorbed serum Enzime-Linked Immunosorbent Assay for diagnosis of bovine paratuberculosis in dairy cows. J Clin Invest 1992;30:613-618.
16. Landis JR, Koch GG: The measurement of ob-server agreement for categorical data. Biometrics 1977;33:159-174.
17. Nielsen SS, Nielsen KK, Huda A, Condron R, Col-lins MT: Diagnostic techniques for paratuberculo-sis. Bull Int Dairy Fed 2001;362:5-15.
18. McKenna SLB, Barkema HW, Keefe GP, Sockett DC: Agreement between three ELISAs for Myco-bacterium avium subsp. paratuberculosis in dairy cattle. Vet Microbiol 2006;114:285-291.
19. Barrington GM, Gay JM, Eriks IS, et al: Temporal patterns of diagnostic results in serial samples from cattle with advanced paratuberculosis infection. J Vet Diagn Invest 2003;15:195-200.
20. Pillai SR, Jayarao BM: Application of IS900 PCR for detection of Mycobacterium avium subsp. para-tuberculosis directly from raw milk. J Dairy Sci 2002;85:1052-1057.
21. Juste RA, Garrido JM, Geijo M, et al: Comparison of blood polymerase chain reaction and enzyme-linked immunosorbent assay for detection of Mycobacterium avium subsp. paratuberculosis infection in cattle and sheep. J Vet Diagn Invest 2005;17:354-359.