Superiority of the buffy coat over serum or plasma for the detection of Alkhumra virus RNA using real time RT-PCR

Archives of Virology

Volumn 157, Issue 5, 2012, Pages 819-823

  Madani, Tariq A ^a   Abuelzein, El Tayeb M E ^b   Azhar, Esam I ^b   Kao, Moujahed ^b   Al Bar, Hussein M S ^a   Abu Araki, Huda ^b   Ksiazek, Thomas G ^c  

^a King Abdulaziz University   (Saudi Arabia)

^b KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

^c UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS RNA;

ADULT; ANIMAL; ARTICLE; BLOOD; BLOOD BUFFY COAT; CELL LINE; CHEMISTRY; CLASSIFICATION; EVALUATION; FEMALE; FLAVIVIRUS; FLAVIVIRUS INFECTION; GENETICS; HAMSTER; HUMAN; ISOLATION AND PURIFICATION; MALE; METHODOLOGY; MIDDLE AGED; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIROLOGY;

ADULT; ANIMALS; BLOOD BUFFY COAT; CELL LINE; CRICETINAE; FEMALE; FLAVIVIRUS; FLAVIVIRUS INFECTIONS; HUMANS; MALE; MIDDLE AGED; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; YOUNG ADULT;

CRICETINAE; MACACA MULATTA;

EID: 84860432850     PISSN: 03048608     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00705-012-1237-7     Document Type: Article

References (11)

1. Qattan I, Akbar N, Afif H, Abu Azmah S, Al-Khateeb T, Zaki A et al (1996) A novel flavivirus: Makkah Region 1994-1996. Saudi Epidemiol Bulletin 3(1): 1-3 ISSN: 1319-3965.
2. Madani TA (2005) Alkhumra virus infection, a new viral hemorrhagic fever in Saudi Arabia. J Infect 51(2): 91-97.
3. Madani TA, Azhar EI, Abuelzein EME, Kao M, Al-Bar HMS, Abu-Araki H et al (2011) Alkhumra (Alkhurma) virus outbreak in Najran, Saudi Arabia: epidemiological, clinical, and laboratory characteristics. J Infect 62(1): 67-76.
4. Carletti F, Castilletti C, Di Caro A, Capobianchi MR, Nisii C, Suter F et al (2010) Alkhurma hemorrhagic fever in travelers returning from Egypt, 2010. Emerg Infect Dis 16(12): 1979-1982.
5. Charrel RN, Zaki AM, Fakeeh M, Yousef AI, de Chesse R, Attoui H et al (2005) Low diversity of Alkhurma hemorrhagic fever virus, Saudi Arabia, 1994-1999. Emerg Infect Dis 11: 683-688.
6. Holland PM, Abramson RD, Watson R, Gelfand DH (1991) Detection of specific polymerase chain reaction product by utilizing the 5′-3′exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 88: 7276-7280.
7. Livak KJ, Flood SJ, Marmaro J, Giusti W, Deetz K (1995) Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 4: 357-362.
8. Drosten C, Göttig S, Schilling S, Asper M, Panning M, Schmitz H et al (2002) Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-RT-PCR. J Clin Microbiol 40(7): 2323-2330.
9. Wang HL, Lin KH, Yueh YY, Chow L, Wu YC, Chen HY et al (2000) Efficient diagnosis of dengue infections using patients peripheral blood leukocytes and serum/plasma. Intervirol 43: 107-111.
10. Scott RM, Nisalak A, Cheamudon U, Seridhoranakul S, Nimmannitya S (1980) Isolation of dengue viruses from peripheral blood leukocytes of patients with haemorrhagic fever. J Infect Dis 141: 1-6.
11. Klungthong C, Gibbson RV, Thaisomboonsuk B, Nisalak A, Kalayanarooj S, Thirawuth V et al (2007) Dengue virus detection using whole blood for reverse transcriptase PCR and virus isolation. J Clinic Microbiol 45(8): 2480-2485.