Membrane fluidity profiles as deduced by saturation-recovery EPR measurements of spin-lattice relaxation times of spin labels

Journal of Magnetic Resonance

Volumn 212, Issue 2, 2011, Pages 418-425

  Mainali, Laxman ^a   Feix, Jimmy B ^a   Hyde, James S ^a   Subczynski, Witold K ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

Author keywords

Membrane fluidity; Saturation recovery; Spin labels; Spin lattice relaxation rate; Spin lattice relaxation time

Indexed keywords

MEMBRANE FLUIDITY; SATURATION-RECOVERY; SPIN LABEL; SPIN-LATTICE RELAXATION RATE; SPIN-LATTICE RELAXATION-TIME;

CHOLESTEROL; COMPUTER SIMULATION; FLUIDITY; LIPID BILAYERS; MEMBRANES; RECOVERY; RELAXATION PROCESSES; RIGID STRUCTURES; SPIN-LATTICE RELAXATION;

ELECTRON SPIN RESONANCE SPECTROSCOPY;

CHOLESTEROL; DIMYRISTOYLPHOSPHATIDYLCHOLINE; PHOSPHOLIPID;

ARTICLE; ARTIFICIAL MEMBRANE; CHEMISTRY; DIFFUSION; ELECTRON SPIN RESONANCE; LIPID BILAYER; MEMBRANE FLUIDITY; METHODOLOGY; NONLINEAR SYSTEM; PHYSIOLOGY; SPIN LABELING; STATISTICAL ANALYSIS;

CHOLESTEROL; DATA INTERPRETATION, STATISTICAL; DIFFUSION; DIMYRISTOYLPHOSPHATIDYLCHOLINE; ELECTRON SPIN RESONANCE SPECTROSCOPY; LIPID BILAYERS; MEMBRANE FLUIDITY; MEMBRANES, ARTIFICIAL; NONLINEAR DYNAMICS; PHOSPHOLIPIDS; SPIN LABELS;

EID: 84860390260     PISSN: 10907807     EISSN: 10960856     Source Type: Journal    
DOI: 10.1016/j.jmr.2011.07.022     Document Type: Article

References (51)

1. W.L. Hubbell, and H.M. McConnell Spin-label studies of the excitable membranes of nerve and muscle Proc. Natl. Acad. Sci. USA 61 1968 12 16
2. D. Marsh Electron spin resonance: spin labels E. Grell, Membr. Spectrosc. 1981 Springer-Verlag Berlin 51 142
3. J.J. Yin, and W.K. Subczynski Effects of lutein and cholesterol on alkyl chain bending in lipid bilayers: a pulse electron spin resonance spin labeling study Biophys. J. 71 1996 832 839 (Pubitemid 26289166)
4. P.F. Devaux ESR and NMR studies of lipid-protein interactions in membranes L.J. Berliner, J. Reuben, Biological Magnetic Resonance 1983 Plenum Press New York 183 299
5. S. Schreier, C.F. Polnaszek, and I.C. Smith Spin labels in membranes: problems in practice Biochim. Biophys. Acta 515 1978 395 436 (Pubitemid 9099586)
6. M.J. Swamy, L. Ciani, M. Ge, A.K. Smith, D. Holowka, B. Baird, and J.H. Freed Coexisting domains in the plasma membranes of live cells characterized by spin-label ESR spectroscopy Biophys. J. 90 2006 4452 4465 (Pubitemid 43830891)
7. M. Ge, A. Gidwani, H.A. Brown, D. Holowka, B. Baird, and J.H. Freed Ordered and disordered phases coexist in plasma membrane vesicles of RBL-2H3 mast cells Biophys. J. 85 2003 1278 1288 (Pubitemid 36909688)
8. L. Kar, E. Ney-Igner, and J.H. Freed Electron spin resonance and electron-spin-echo study of oriented multilayers of L alpha- dipalmitoylphosphatidylcholine water systems Biophys. J. 48 1985 569 595
9. B.H. Robinson, D.A. Haas, and C. Mailer Molecular dynamics in liquids: spin-lattice relaxation of nitroxide spin labels Science 263 1994 490 493 (Pubitemid 24082747)
10. C. Mailer, R.D. Nielsen, and B.H. Robinson Explanation of spin-lattice relaxation rates of spin labels obtained with multifrequency saturation recovery EPR J. Phys. Chem. A 109 2005 4049 4061 (Pubitemid 40750859)
11. W.K. Subczynski, M. Raguz, and J. Widomska Studying lipid organization in biological membranes using liposomes and EPR spin labeling Methods Mol. Biol. 606 2010 247 269
12. I. Ashikawa, J.J. Yin, W.K. Subczynski, T. Kouyama, J.S. Hyde, and A. Kusumi Molecular organization and dynamics in bacteriorhodopsin-rich reconstituted membranes: discrimination of lipid environments by the oxygen transport parameter using a pulse ESR spin-labeling technique Biochemistry 33 1994 4947 4952 (Pubitemid 24158446)
13. W.K. Subczynski, A. Wisniewska, J.S. Hyde, and A. Kusumi Three-dimensional dynamic structure of the liquid-ordered domain in lipid membranes as examined by pulse-EPR oxygen probing Biophys. J. 92 2007 1573 1584 (Pubitemid 46393468)
14. A. Kusumi, W.K. Subczynski, and J.S. Hyde Oxygen transport parameter in membranes as deduced by saturation recovery measurements of spin-lattice relaxation times of spin labels Proc. Natl. Acad. Sci. USA 79 1982 1854 1858 (Pubitemid 12088526)
15. W.K. Subczynski, J.S. Hyde, and A. Kusumi Effect of alkyl chain unsaturation and cholesterol intercalation on oxygen transport in membranes: a pulse ESR spin labeling study Biochemistry 30 1991 8578 8590
16. M. Raguz, L. Mainali, J. Widomska, and W.K. Subczynski The immiscible cholesterol bilayer domain exists as an integral part of phospholipid bilayer membranes Biochim. Biophys. Acta 1808 2011 1072 1080
17. J.S. Hyde, J.J. Yin, W.K. Subczynski, T.G. Camenisch, J.J. Ratke, and W. Froncisz Spin-label EPR T1 values using saturation recovery from 2 to 35 GHz J. Phys. Chem. B 108 2004 9524 9529
18. W. Froncisz, T.G. Camenisch, J.J. Ratke, J.R. Anderson, W.K. Subczynski, R.A. Strangeway, J.W. Sidabras, and J.S. Hyde Saturation recovery EPR and ELDOR at W-band for spin labels J. Magn. Reson. 193 2008 297 304
19. W.K. Subczynski, L. Mainali, T.G. Camenisch, W. Froncisz, and J.S. Hyde Spin-label oximetry at Q- and W-band J. Magn. Reson. 209 2011 142 148
20. A. Kusumi, W.K. Subczynski, M. Pasenkiewicz-Gierula, J.S. Hyde, and H. Merkle Spin-label studies on phosphatidylcholine-cholesterol membranes: effects of alkyl chain length and unsaturation in the fluid phase Biochim. Biophys. Acta 854 1986 307 317 (Pubitemid 16149085)
21. W.K. Subczynski, C.C. Felix, C.S. Klug, and J.S. Hyde Concentration by centrifugation for gas exchange EPR oximetry measurements with loop-gap resonators J. Magn. Reson. 176 2005 244 248 (Pubitemid 41336693)
22. A. Wisniewska, Y. Nishimoto, J.S. Hyde, A. Kusumi, and W.K. Subczynski Depth dependence of the perturbing effect of placing a bulky group (oxazolidine ring spin labels) in the membrane on the membrane phase transition Biochim. Biophys. Acta 1278 1996 68 72 (Pubitemid 26042272)
23. P.F.F. Almeida, W.L.C. Vaz, and T.E. Thompson Lateral diffusion in the liquid phases of dimyristoylphosphatidylcholine/cholesterol lipid bilayers: a free volume analysis Biochemistry 31 1992 6739 6747
24. C.A. Popp, and J.S. Hyde Electron-electron double resonance and saturation-recovery studies of nitroxide electron and nuclear spin-lattice relaxation times and Heisenberg exchange rates: lateral diffusion in dimyristoyl phosphatidylcholine Proc. Natl. Acad. Sci. USA 79 1982 2559 2563
25. J.J. Yin, J.B. Feix, and J.S. Hyde Solution of the nitroxide spin-label spectral overlap problem using pulse electron spin resonance Biophys. J. 53 1988 525 531 (Pubitemid 18095300)
26. W.K. Subczynski, J. Widomska, A. Wisniewska, and A. Kusumi Saturation-recovery electron paramagnetic resonance discrimination by oxygen transport (DOT) method for characterizing membrane domains T.J. McIntosh, Methods in Molecular Biology, "Lipid Rafts" 2007 Humana Press Totowa 143 157
27. D.E. Budil, S. Lee, S. Saxena, and J.H. Freed Nonlinear-least-squares analysis of slow-motion EPR spectra in one and two dimensions using a modified Levenberg-Marquardt algorithm J. Magn. Reson. 120 1996 155 189 (Pubitemid 126751752)
28. E. Meirovitch, D. Igner, G. More, and J.H. Freed Electron-spin relaxation and ordering in smectic and supercooled nematic liquid crystals J. Chem. Phys. 77 1982 3915 3938
29. D.J. Schneider, and J.H. Freed Calculating slow motional magnetic resonance spectra: a user's guide L.J. Berliner, J. Reuben, Spin Labeling: Theory and Application 1989 Plenum New York 1 76
30. K.A. Earle, J.K. Moscicki, M. Ge, D.E. Budil, and J.H. Freed 250-GHz electron spin resonance studies of polarity gradients along the aliphatic chains in phospholipid membranes Biophys. J. 66 1994 1213 1221 (Pubitemid 24106368)
31. D. Kurad, G. Jeschke, and D. Marsh Lipid membrane polarity profiles by high-field EPR Biophys. J. 85 2003 1025 1033 (Pubitemid 36909666)
32. E. Meirovitch, and J.H. Freed Analysis of slow-motional electron spin resonance spectra in smectic phases in terms of molecular configuration, intermolecular interactions, and dynamics J. Phys. Chem. 88 1984 4995 5004
33. K.A. Earle, and D.E. Budil Calculating slow-motion ESR spectra of spin-labeled polymers S. Schlick, Advanced ESR Methods in Polymer Research 2006 John Wiley & Sons Hoboken, NJ 54 82
34. A. Smirnov, R. Belford, and R. Clarkson Comparative spin label spectra at X- band and W- band L.J. Berliner, Biological Magnetic Resonance Spin Labeling: The Next Millennium vol. 14 1998 Plenum Press New York, NY 83 107
35. W.L. Hubbell, and H.M. McConnell Molecular motion in spin-labeled phospholipids and membranes J. Am. Chem. Soc. 93 1971 314 326
36. T. Rog, and M. Pasenkiewicz-Gierula Cholesterol effects on the phosphatidylcholine bilayer nonpolar region: A molecular simulation study Biophys. J. 81 2001 2190 2202 (Pubitemid 32917168)
37. T. Rog, M. Pasenkiewicz-Gierula, I. Vattulainen, and M. Karttunen Ordering effects of cholesterol and its analogues Biochim. Biophys. Acta 1788 2009 97 121
38. B.J. Gaffney Practical considerations for the calculation of order parameters for fatty acid or phospholipid spin labels in membranes L.J. Berliner, Spin Labeling: Theory and Applications 1976 Academic Press New York, NY 567 571
39. O.H. Griffith, and P.C. Jost Lipid spin labels in biological membranes L.J. Berliner, Spin Labeling: Theory and Applications 1976 Academic Press New York, NY 453 523
40. C.F. Polnaszek, and J.H. Freed Electron spin resonance studies of anisotropic ordering, spin relaxation, and slow tumbling in liquid-crystalline solvents J. Phys. Chem. 79 1975 2283 2306
41. Y.W. Chiang, Y. Shimoyama, G.W. Feigenson, and J.H. Freed Dynamic molecular structure of DPPC-DLPC-cholesterol ternary lipid system by spin-label electron spin resonance Biophys. J. 87 2004 2483 2496
42. J. Widomska, M. Raguz, J. Dillon, E.R. Gaillard, and W.K. Subczynski Physical properties of the lipid bilayer membrane made of calf lens lipids: EPR spin labeling studies Biochim. Biophys. Acta 1768 2007 1454 1465 (Pubitemid 46782711)
43. M. Raguz, J. Widomska, J. Dillon, E.R. Gaillard, and W.K. Subczynski Physical properties of the lipid bilayer membrane made of cortical and nuclear bovine lens lipids: EPR spin-labeling studies Biochim. Biophys. Acta 1788 2009 2380 2388
44. J.S. Hyde, and W.K. Subczynski Spin-label oximetry L.J. Berliner, J. Reuben, Biological Magnetic Resonance 1989 Plenum Press New York 399 425
45. W.K. Subczynski, and H.M. Swartz EPR oximetry in biological and model samples S.S. Eaton, G.R. Eaton, L.J. Berliner, Biological Magnetic Resonance, Biomedical EPR - Part A: Free Radicals, Metals, Medicine, and Physiology 2005 Kluwer/Plenum New York 229 282
46. W.K. Subczynski, J.S. Hyde, and A. Kusumi Oxygen permeability of phosphatidylcholine-cholesterol membranes Proc. Natl. Acad. Sci. USA 86 1989 4474 4478 (Pubitemid 19164577)
47. J.J. Yin, and J.S. Hyde Application of rate equations to ELDOR and saturation recovery experiments on 14N:15N spin-label pairs J. Magn. Reson. 74 1987 82 93
48. J.J. Yin, J.B. Feix, and J.S. Hyde Mapping of collision frequencies for stearic acid spin labels by saturation-recovery electron paramagnetic resonance Biophys. J. 58 1990 713 720 (Pubitemid 20312928)
49. J.J. Yin, J.B. Feix, and J.S. Hyde The effects of cholesterol on lateral diffusion and vertical fluctuations in lipid bilayers, An electron-electron double resonance (ELDOR) study Biophys. J. 52 1987 1031 1038
50. W.K. Subczynski, A. Wisniewska, J.J. Yin, J.S. Hyde, and A. Kusumi Hydrophobic barriers of lipid bilayer membranes formed by reduction of water penetration by alkyl chain unsaturation and cholesterol Biochemistry 33 1994 7670 7681 (Pubitemid 24230593)
51. D. Marsh Polarity and permeation profiles in lipid membranes Proc. Natl. Acad. Sci. USA 98 2001 7777 7782 (Pubitemid 32642637)