Purification and biochemical properties of a Kunitz-type trypsin inhibitor from Entada acaciifolia (Benth.) seeds

Process Biochemistry

Volumn 47, Issue 6, 2012, Pages 929-935

  De Oliveira, Caio Fernando Ramalho ^a,d   Vasconcelos, Ilka Maria ^b   Aparicio, Ricardo ^a   Freire, Maria Das Graças MacHado ^c   Baldasso, Paulo Aparecido ^a   Marangoni, Sergio ^a   MacEdo, Maria Lígia Rodrigues ^d  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b University of Ceará CE   (Brazil)

^c CENSA Higher Institutes   (Brazil)

^d FEDERAL UNIVERSITY OF MATO GROSSO DO SUL   (Brazil)

Author keywords

Circular dichroism; Entada acaciifolia; Kunitz type inhibitor; Trypsin inhibitor

Indexed keywords

AMINO ACID COMPOSITIONS; BIOCHEMICAL PROPERTIES; CONFORMATIONAL STABILITIES; DISSOCIATION CONSTANT; DISULFIDE BONDS; ENTADA ACACIIFOLIA; HIGH AFFINITY; INHIBITORY ACTIVITY; KUNITZ-TYPE INHIBITOR; KUNITZ-TYPE TRYPSIN INHIBITOR; PH STABILITY; REACTIVE SITE; RP-HPLC; STRUCTURAL MODIFICATIONS; TRYPSIN INHIBITOR;

AMINO ACIDS; DICHROISM; DISSOCIATION; STOICHIOMETRY; UREA;

COVALENT BONDS;

BOVINAE; ENTADA;

EID: 84860377050     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2012.02.022     Document Type: Article

References (48)

1. B. Bijina, S. Chellappan, S.M. Basheer, K.K. Elyas, A.H. Bahkali, and M. Chandrasekaran Protease inhibitor from Moringa oleifera leaves: isolation, purification, and characterization Proc Biochem 46 2011 2291 2300
2. M. Macedo, S. Pando, L. Chevreuil, and S. Marangoni Properties of a Kunitz-type trypsin inhibitor from Delonix regia seeds against digestive proteinases of Anagasta kuehniella (Z.) and Corcyra cephalonica (S.) (Lepidoptera: Pyralidae) Protein Peptide Lett 16 2009 1459 1465
3. C.R. Carlini, and M.F. Grossi-de-Sá Plant toxic proteins with insecticidal properties. A review on their potentialities as bioinsecticides Toxicon 40 2002 1515 1539
4. P.K. Lawrence, and K.R. Koundal Plant protease inhibitors in control of phytophagous insects Eletron J Biotechnol 5 2002 5 6
5. K.Y. Ee, J. Zhao, A. Rehman, and S.O. Agboola Purification and characterization of a Kunitz-type trypsin inhibitor from Acacia victoriae Bentham Seeds J Agric Food Chem 57 2009 7022 7029
6. G. Norton Proteinase inhibitors J.P.F. D'Mello, C.M. Duffus, J.H. Duffus, Toxic Substances in Crop Plants 1991 The Royal Society of Chemistry Cambridge 68 106
7. S.K. Haq, S.M. Atif, and R.H. Khan Protein proteinase inhibitor genes in combat against insects, pests, and pathogens: natural and engineered phytoprotection Arch Biochem Biophys 431 2004 145 159
8. N.P. Chougule, E. Doyle, E. Fitches, and J.A. Gatehouse Biochemical characterization of midgut digestive proteases from Mamestra brassicae (cabbage moth; Lepidoptera: Noctuidae) and effect of soybean Kunitz inhibitor (SKTI) in feeding assays J Insect Physiol 54 2008 563 572
9. N. Ferry, L. Jouanin, L.R. Ceci, E.A. Mulligan, K. Emami, and J.A. Gatehouse Impact of oilseed rape expressing the insecticidal serine protease inhibitor, mustard trypsin inhibitor-2 on the beneficial predator Pterostichus madidus Mol Ecol 14 2005 337 349
10. M. Trovato, E.C. Casavola, B. Maras, M.E. Schininà, P. Costantino, and P. Ascenzi Protein minimization: characterization of the synthetic cyclic dodecapeptide corresponding to the reactive site region of the oil rape trypsin inhibitor type-III Biochem Biophys Res Commun 302 2003 311 315
11. M. Trovato, B. Maras, F. Polticelli, P. Costantino, and P. Ascenzi A chimeric mini-trypsin inhibitor derived from the oil rape proteinase inhibitor type III Biochem Biophys Res Commun 275 2000 817 820
12. J.A. Torres-Castillo, C.M. Jacobo, and A. Blanco-Labra Characterization of a highly stable trypsin-like proteinase inhibitor from the seeds of Opuntia streptacantha (O. streptacantha Lemaire) Phytochemistry 70 2009 1374 1381
13. A.V. Konarev, A. Lovegrove, and P.R. Shewry Serine proteinase inhibitors in seeds of Cycas siamensis and other gymnosperms Phytochemistry 69 2008 2482 2489
14. M.H. Lingaraju, and L.R. Gowda A Kunitz trypsin inhibitor of Entada scandens seeds: another member with single disulfide bridge BBA Proteins Proteom 1784 2008 850 855
15. N.R. Bueno, H. Fritz, E.A. Auerswald, R. Mentele, M. Sampaio, and C.A.M. Sampaio Primary structure of Dioclea glabra trypsin inhibitor, DgTI, a Bowman-Birk inhibitor Biochem Biophys Res Commun 261 1999 838 843
16. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 1976 248 254
17. U. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
18. R.L. Henrison, and S.C. Meredith Amino acid analysis by reverse phase high performance liquid chromatoghraphy: precolumn derivatization with phenyliosothiocyanate Anal Biochem 136 1984 65 71
19. M.R. Wilkins, E. Gasteiger, A. Bairoch, J.C. Sanchez, K.L. Williams, and R.D. Appel Protein identification and analysis tools in the ExPASy server A.J. Link, 2-D Proteome Analysis Protocols 1998 Humana Press Towana
20. S.F. Altschul, W. Gish, W. Miller, E.W. Myers, and D.J. Lipman Basic local alignment search tool J Mol Biol 215 1990 403 410
21. D.G. Higgins, J.D. Thompson, and T.J. Gibson Using CLUSTAL for multiple sequence alignments F.D. Russell, Methods in Enzymology vol. 266 1996 Academic Press 383 402
22. B. Elanger, N. Nokowsky, and W. Cohen The preparation and properties of two chromogenic substrates of trypsin Arch Biochem Biophys 95 1961 271 278
23. L. Whitmore, and B.A. Wallace DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data Nucleic Acids Res 32 2004 668 673
24. S.W. Provencher, and J. Glockner Estimation of globular protein secondary structure from circular dichroism Biochemistry-US 20 1981 33 37
25. T.A. Valueva, T.A. Revina, and V.V. Mosolov Potato tuber protein proteinase inhibitors belonging to the Kunitz soybean inhibitor family Biochemistry-US 62 1997 1367 1374
26. M. Macedo, R. Durigan, D. da Silva, S. Marangoni, M. Freire, and J. Parra Adenanthera pavonina trypsin inhibitor retard growth of Anagasta kuehniella (Lepidoptera: Pyralidae) Arch Insect Biochem Physiol 73 2010 213 231
27. F.J. Joubert, F.H.H. Carlsson, and T. Haylett Purification and some properties of two proteinase inhibitors (DE-1 and DE-3) from Erythrina latissima (Broad-Leaved Erythrina) Seed Hoppe-Seyler's Zeitschrift für physiologische Chemie 362 1981 531 538
28. M. Macedo, V. Garcia, M. Freire, and M. Richardson Characterization of a Kunitz trypsin inhibitor with a single disulfide bridge from seeds of Inga laurina (SW.) Willd Phytochemistry 68 2007 1104 1111
29. L. Migliolo, A.S. de Oliveira, E.A. Santos, O.L. Franco, and M.P. de Sales Structural and mechanistic insights into a novel non-competitive Kunitz trypsin inhibitor from Adenanthera pavonina L. seeds with double activity toward serine- and cysteine-proteinases J Mol Graph Model 29 2010 148 156
30. A. Bhattacharyya, S. Mazumdar, S. Leighton, and C. Babu A Kunitz proteinase inhibitor from Archidendron ellipticum seeds: purification, characterization, and kinetic properties Phytochemistry 67 2006 232 241
31. A. Bhattacharyya, and C. Babu Purification and biochemical characterization of a serine proteinase inhibitor from Derris trifoliata Lour. seeds: insight into structural and antimalarial features Phytochemistry 70 2009 703 712
32. M. Richardson, F.A.P. Campos, J. Xavier-Filho, M.L.R. Macedo, G.M.C. Maia, and A. Yarwood The amino acid sequence and reactive (inhibitory) site of the major trypsin isoinhibitor (DE5) isolated from seeds of the Brazilian Carolina tree (Adenanthera pavonina L.) BBA-Protein Struct M 872 1986 134 140
33. L.C. Gruen, Z.J. Tao, and A.A. Kort Stability and physicochemical properties of a trypsin inhibitor from winged bean seed (Psophocarpus tetragonolobus (L)DC) Biochim Biophys Acta 791 1984 285 293
34. Y. Kouzuma, M. Suetake, M. Kimura, and N. Yamasaki Isolation and primary structure of proteinase inhibitors from Erythrina variegata (Linn.) var. Orientalis seeds Biosci Biotechnol Biochem 56 1992 1819 1824
35. H.-C. Wu, and J.-Y. Lin The complete amino acid sequence of a Kunitz family trypsin inhibitor from seeds of Acacia confusa J Biochem 113 1993 258 263
36. L.A. Oliveira Cd Santana, A.K. Carmona, M.H. Cezari, M.U. Sampaio, C.A.M. Sampaio, and M.L.V. Oliva Structure of Cruzipain/Cruzain inhibitors isolated from Bauhinia bauhinioides seeds Biol Chem 382 2001 847 852
37. I.F.C. Batista, M.L.V. Oliva, M.S. Araujo, M.U. Sampaio, M. Richardson, and H. Fritz Primary structure of a Kunitz-type trypsin inhibitor from Enterolobium contortisiliquum seeds Phytochemistry 41 1996 1017 1022
38. A.N. Negreiros, M.M. Carvalho, J. Xavier-Filho, A. Blanco-Labra, P.R. Shewry, and M. Richardson The complete amino acid sequence of the major Kunitz trypsin inhibitor from the seeds of Prosopsis juliflora Phytochemistry 30 1991 2829 2833
39. M. Macedo, E. Diz Filho, M. Freire, M. Oliva, J. Sumikawa, and M. Toyama A trypsin inhibitor from Sapindus saponaria L. seeds: purification, characterization, and activity towards pest insect digestive enzyme Protein J 30 2011 9 19
40. Y. Liu, K. Breslauer, and S. Anderson Designing out disulfide bonds: thermodynamic properties of 30-51 cystine substitution mutants of bovine pancreatic trypsin inhibitor Biochemistry-US 36 1997 5323 5335
41. M. Zavodszky, C.-W. Chen, J.-K. Huang, M. Zolkiewski, L. Wen, and R. Krishnamoorthi Disulfide bond effects on protein stability: designed variants of Cucurbita maxima trypsin inhibitor-V Protein Sci 10 2001 149 160
42. N. Chaudhary, C. Shee, A. Islam, F. Ahmad, D. Yernool, and P. Kumar Purification and characterization of a trypsin inhibitor from Putranjiva roxburghii seeds Phytochemistry 69 2008 2120 2126
43. K. Lehle, U. Kohnert, A. Stern, F. Popp, and R. Jaenicke Effect of disulfide bonds on the structure, function, and stability of the trypsin/tPA inhibitor from Erythrina caffra: site-directed mutagenesis, expression, and physiochemical characterization Nat Biotechnol 14 1996 476 480
44. S. Onesti, P. Brick, and D.M. Blow Crystal structure of a Kunitz-type trypsin inhibitor from Erythrina coffra seeds J Mol Biol 217 1991 153 176
45. R.M. Sweet, H.T. Wright, J. Janin, C.H. Chothia, and D.M. Blow Crystal structure of the complex of porcine trypsin with soybean trypsin inhibitor (Kunitz) at 2.6 resolution Biochemistry-US 13 1974 4212 4228
46. M. Azarkan, R. Dibiani, E. Goormaghtigh, V. Raussens, and D. Baeyens-Volant The papaya Kunitz-type trypsin inhibitor is a highly stable β-sheet glycoprotein BBA-Proteins Proteom 1764 2006 1063 1072
47. D. Hansen, S. Macedo-Ribeiro, P. Veríssimo, S. Yoo Im, M.U. Sampaio, and M.L.V. Oliva Crystal structure of a novel cysteinless plant Kunitz-type protease inhibitor Biochem Biophys Res Commun 360 2007 735 740
48. R.F. Steiner, and H. Edelhoch Structural transitions of soybean trypsin inhibitor. The native protein in water J Biol Chem 238 1963 925 930