Analysis of alternative splicing of cassette exons at single-cell level using two fluorescent proteins

Nucleic Acids Research

Volumn 40, Issue 8, 2012, Pages

  Gurskaya, Nadya G ^a   Staroverov, Dmitry B ^a   Zhang, Lijuan ^a,b   Fradkov, Arkady F ^a   Markina, Nadezhda M ^a   Pereverzev, Anton P ^a   Lukyanov, Konstantin A ^a  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b UNIVERSITY OF VIENNA   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; PROTEIN P53; RED FLUORESCENT PROTEIN;

ALTERNATIVE RNA SPLICING; ARTICLE; CONTROLLED STUDY; EXON; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENE CASSETTE; GENE CONTROL; GENE EXPRESSION; GENE MUTATION; GENE SEQUENCE; GENE TARGETING; HUMAN; HUMAN CELL; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; ULTRAVIOLET IRRADIATION;

ALTERNATIVE SPLICING; EXONS; FLOW CYTOMETRY; FLUORESCENT DYES; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PROTO-ONCOGENE PROTEINS; SINGLE-CELL ANALYSIS;

EID: 84860363361     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkr1314     Document Type: Article

References (17)

1. Blencowe, B.J. (2006) Alternative splicing: new insights from global analyses. Cell, 126, 37-47.
2. Shin, C. and Manley, J.L. (2004) Cell signalling and the control of pre-mRNA splicing. Nat. Rev. Mol. Cell. Biol., 5, 727-738.
3. Barash, Y., Calarco, J.A., Gao, W., Pan, Q., Wang, X., Shai, O., Blencowe, B.J. and Frey, B.J. (2010) Deciphering the splicing code. Nature, 465, 53-59.
4. Wang, Z., Rolish, M.E., Yeo, G., Tung, V., Mawson, M. and Burge, C.B. (2004) Systematic identification and analysis of exonic splicing silencers. Cell, 119, 831-845.
5. Kuroyanagi, H., Kobayashi, T., Mitani, S. and Hagiwara, M. (2006) Transgenic alternative-splicing reporters reveal tissue-specific expression profiles and regulation mechanisms in vivo. Nat. Methods, 3, 909-915.
6. Newman, E.A., Muh, S.J., Hovhannisyan, R.H., Warzecha, C.C., Jones, R.B., McKeehan, W.L. and Carstens, R.P. (2006) Identification of RNA-binding proteins that regulate FGFR2 splicing through the use of sensitive and specific dual color fluorescence minigene assays. RNA, 12, 1129-1141.
7. Orengo, J.P., Bundman, D. and Cooper, T.A. (2006) A bichromatic fluorescent reporter for cell based screens of alternative splicing. Nucleic Acids Res., 34, e148.
8. Ohno, G., Hagiwara, M. and Kuroyanagi, H. (2008) STAR family RNA-binding protein ASD-2 regulates developmental switching of mutually exclusive alternative splicing in vivo. Genes Dev., 22, 360-374.
9. Stoilov, P., Lin, C.H., Damoiseaux, R., Nikolic, J. and Black, D.L. (2008) A high-throughput screening strategy identifies cardiotonic steroids as alternative splicing modulators. Proc. Natl Acad. Sci. USA, 105, 11218-11223.
10. Nicholls, C.D. and Beattie, T.L. (2008) Multiple factors influence the normal and UV-inducible alternative splicing of PIG3. Biochim. Biophys. Acta, 1779, 838-849.
11. Gurskaya, N.G., Staroverov, D.B., Fradkov, A.F. and Lukyanov, K.A. (2011) Coding region of far-red fluorescent protein Katushka contains a strong donor splice site. Russ. J. Bioorg. Chem., 37, 380-382.
12. Colman-Lerner, A., Gordon, A., Serra, E., Chin, T., Resnekov, O., Endy, D., Pesce, C.G. and Brent, R. (2005) Regulated cell-to-cell variation in a cell-fate decision system. Nature, 437, 699-706.
13. Chang, H.H., Hemberg, M., Barahona, M., Ingber, D.E. and Huang, S. (2008) Transcriptome-wide noise controls lineage choice in mammalian progenitor cells. Nature, 453, 544-547.
14. Altschuler, S.J. and Wu, L.F. (2010) Cellular heterogeneity: do differences make a difference? Cell, 141, 559-563.
15. Slack, M.D., Martinez, E.D., Wu, L.F. and Altschuler, S.J. (2008) Characterizing heterogeneous cellular responses to perturbations. Proc. Natl Acad. Sci. USA, 105, 19306-19311.
16. Singh, D.K., Ku, C.J., Wichaidit, C., Steinenger, R.J.I., Wu, L.F. and Altschuler, S.J. (2010) Patterns of basal signaling heterogeneity can distinguish cellular populations with different drug sensitivities. Mol. Syst. Biol, 6, 369.
17. Lewis, B.P., Green, R.E. and Brenner, S.E. (2003) Evidence for the widespread coupling of alternative splicing and nonsense-mediated mRNA decay in humans. Proc. Natl Acad. Sci. USA, 100, 189-192.