Optimized expression and purification for high-activity preparations of algal [FeFe]-hydrogenase

PLoS ONE

Volumn 7, Issue 4, 2012, Pages

  Yacoby, Iftach ^a,c   Tegler, Lotta Tollstoy ^a   Pochekailov, Sergii ^a,c   Zhang, Shuguang ^a   King, Paul W ^b  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b NATIONAL RENEWABLE ENERGY LABORATORY   (United States)

^c TEL AVIV UNIVERSITY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

FD HYDA1 FUSION PROTEIN; HYBRID PROTEIN; HYDA1 HYDROGENASE; HYDROGEN; OXIDOREDUCTASE; UNCLASSIFIED DRUG; BLOOD CLOTTING FACTOR 10A; FERREDOXIN; HYDROGENASE; IRON HYDROGENASE; IRON SULFUR PROTEIN; PROTEINASE; TEV PROTEASE;

AFFINITY CHROMATOGRAPHY; ALGA; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; CULTURE OPTIMIZATION; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; NONHUMAN; PROTEIN DEGRADATION; PROTEIN EXPRESSION; CHLAMYDOMONAS REINHARDTII; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PLASMID;

ALGAE; TOBACCO ETCH VIRUS;

CHLAMYDOMONAS REINHARDTII; ENDOPEPTIDASES; ESCHERICHIA COLI; FACTOR XA; FERREDOXINS; HYDROGENASE; IRON-SULFUR PROTEINS; PLASMIDS; RECOMBINANT FUSION PROTEINS;

EID: 84860362239     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0035886     Document Type: Article

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