Intramolecular fluorescence resonance energy transfer (FRET) sensors of the orexin OX1 and OX2 receptors identify slow kinetics of agonist activation

Journal of Biological Chemistry

Volumn 287, Issue 18, 2012, Pages 14937-14949

  Xu, Tian Rui ^a   Ward, Richard J ^a   Pediani, John D ^a   Milligan, Graeme ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINE RECEPTOR; ACTIVATION PROCESS; C-TERMINAL TAIL; FLUORESCENCE RESONANCE ENERGY TRANSFER; G PROTEIN COUPLED RECEPTORS; INDIVIDUAL CELLS; INTRACELLULAR LOOP; MAP KINASE; MULTI-STEP; OREXIN-B; RANK ORDER; RECEPTOR ACTIVATION; RESPONSE KINETICS; SEQUENCE VARIATIONS; SINGLE RESIDUE; WILD TYPES;

CELL MEMBRANES; ENERGY TRANSFER; FLUORESCENCE; KINETICS; LIGANDS; PEPTIDES; PHOSPHORYLATION;

SENSORS;

CALCIUM ION; G PROTEIN COUPLED RECEPTOR; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MUSCARINIC M3 RECEPTOR; OREXIN 1 RECEPTOR; OREXIN 2 RECEPTOR; OREXIN A; OREXIN B;

ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CELL STRAIN HEK293; CONCENTRATION RESPONSE; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE MUTATION; HUMAN; HUMAN CELL; INTERNALIZATION; INTRAMOLECULAR FLUORESCENCE RESONANCE ENERGY TRANSFER; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; RECEPTOR BINDING; RECEPTOR UPREGULATION; TIME; WILD TYPE;

AMINO ACID SUBSTITUTION; BIOSENSING TECHNIQUES; FLUORESCENCE RESONANCE ENERGY TRANSFER; HEK293 CELLS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; KINETICS; MAP KINASE SIGNALING SYSTEM; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; MUTATION, MISSENSE; NEUROPEPTIDES; PEPTIDES; PHOSPHORYLATION; PROTEIN STRUCTURE, SECONDARY; RECEPTOR, MUSCARINIC M3; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, NEUROPEPTIDE;

EID: 84860354370     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.334300     Document Type: Article

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