Outer dynein arm light chain 1 is essential for controlling the ciliary response to cyclic AMP in Paramecium tetraurelia

Eukaryotic Cell

Volumn 11, Issue 5, 2012, Pages 645-653

  Kutomi, Osamu ^a   Hori, Manabu ^b,c   Ishida, Masaki ^d   Tominaga, Takashi ^e   Kamachi, Hiroyuki ^a   Koll, France ^c   Cohen, Jean ^c   Yamada, Norico ^a   Noguchia, Munenori ^a  

^a UNIVERSITY OF TOYAMA   (Japan)

^b YAMAGUCHI UNIVERSITY   (Japan)

^c CNRS   (France)

^d NARA UNIVERSITY OF EDUCATION   (Japan)

^e TOKUSHIMA BUNRI UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM CHLORIDE; CYCLIC AMP; DYNEIN ADENOSINE TRIPHOSPHATASE; MESSENGER RNA; PROTOZOAL PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; AXONEME; CULTURE TECHNIQUE; DRUG EFFECT; EUKARYOTIC FLAGELLUM; GENE; GENETICS; LOCOMOTION; METABOLISM; MOLECULAR GENETICS; PARAMECIUM TETRAURELIA; PHENOTYPE; PHOSPHORYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; RNA INTERFERENCE;

AMINO ACID SEQUENCE; AXONEME; CALCIUM CHLORIDE; CILIA; CULTURE TECHNIQUES; CYCLIC AMP; DYNEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENES, PROTOZOAN; LOCOMOTION; MOLECULAR SEQUENCE DATA; PARAMECIUM TETRAURELIA; PHENOTYPE; PHOSPHORYLATION; PROTOZOAN PROTEINS; RNA INTERFERENCE; RNA, MESSENGER;

CHLAMYDOMONAS REINHARDTII; PARAMECIUM TETRAURELIA;

EID: 84860316583     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.05279-11     Document Type: Article

References (45)

1. Arnaiz O, Sperling L. 2011. ParameciumDB in 2011: new tools and new data for functional and comparative genomics of the model ciliate Paramecium tetraurelia. Nucleic Acids Res. 39:D632-D636.
2. Arnaiz O, Cain S, Cohen J, Sperling L. 2007. ParameciumDB: a community resource that integrates the Paramecium tetraurelia genome sequence with genetic data. Nucleic Acids Res. 35:D439-D444.
3. Arnaiz O, et al. 2009. Cildb: a knowledgebase for centrosomes and cilia. Database 2009:bap022. doi:10.1093/database/bap022.
4. Aury JM, et al. 2006. Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia. Nature 444:171-178.
5. Barkalow K, Hamasaki T, Satir P. 1994. Regulation of 22S dynein by a 29-kD light chain. J. Cell Biol. 126:727-735.
6. Baron DM, Kabututu ZP, Hill KL. 2007. Stuck in reverse: loss of LC1 in Trypanosoma brucei disrupts outer dynein arms and leads to reverse flagellar beat and backward movement. J. Cell Sci. 120:1513-1520.
7. Becker-André M, Hahlbrock K. 1989. Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY). Nucleic Acids Res. 17:9437-9446.
8. Benashski SE, Patel-King RS, King SM. 1999. Light chain 1 from the Chlamydomonas outer dynein arm is a leucine-rich repeat protein associated with the motor domain of the γ heavy chain. Biochemistry 38:7253-7264.
9. Blom N, Gammeltoft S, Brunak S. 1999. Sequence-and structure-based prediction of eukaryotic protein phosphorylation sites. J. Mol. Biol. 294: 1351-1362.
10. Blum H, Beier H, Gross HJ. 1987. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8:93-99.
11. Bonini NM, Nelson DL. 1988. Differential regulation of Paramecium ciliary motility by cAMP and cGMP. J. Cell Biol. 106:1615-1623.
12. Brokaw CJ, Kamiya R. 1987. Bending patterns of Chlamydomonas flagella. IV. Mutants with defects in inner and outer dynein arms indicate differences in dynein arm function. Cell Motil. Cytoskeleton 8:68-75.
13. Eckert R. 1972. Bioelectric control of ciliary activity. Science 176:473-481.
14. Galvani A, Sperling L. 2002. RNA interference by feeding in Paramecium. Trends Genet. 18:11-12.
15. Gilliland G, Perrin S, Blanchard K, Bunn HF. 1990. Analysis of cytokine mRNA and DNA, detection and quantitation by competitive polymerase chain reaction. Proc. Natl. Acad. Sci. U. S. A. 87:2725-2729.
16. Gogendeau D, et al. 2008. Functional diversification of centrins and cell morphological complexity. J. Cell Sci. 121:65-74.
17. Hamasaki T, Barkalow K, Richmond J, Satir P. 1991. cAMP stimulated phosphorylation of an axonemal polypeptide that copurifies with the 22S dynein arm regulates microtubule translocation velocity and swimming speed in Paramecium. Proc. Natl. Acad. Sci. U. S. A. 88:7918-7922.
18. Hamasaki T, Murtaugh TJ, Satir BH, Satir P. 1989. In vitro phosphorylation of Paramecium axonemes and permeabilized cells. Cell Motil. Cytoskeleton 12:1-11.
19. King SM, Kamiya R. 2008. Axonemal dyneins: assembly, structure and force generation, p 131-208. In Witman GB (ed), The Chlamydomonas sourcebook: cell motility and behavior, vol 3. Academic Press, San Diego, CA.
20. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
21. Lowry OH, Rowebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
22. Machemer H. 1988. Electrophysiology, p 185-215. In Görtz HD (ed), Paramecium. Springer-Verlag, Berlin, Germany.
23. Mogami Y, Takahashi K. 1983. Calcium and microtubule sliding in ciliary axonemes isolated from Paramecium caudatum. J. Cell Sci. 61:107-121.
24. Naitoh Y, Kaneko H. 1972. Reactivated Triton-extracted models of Paramecium: modification of ciliary movement by calcium ions. Science 176: 523-524.
25. Nakaoka Y, Ooi H. 1985. Regulation of ciliary reversal in Tritonextracted Paramecium by calcium and cyclic adenosine monophosphate. J. Cell Sci. 77:185-195.
26. Noguchi M, Kitani T, Ogawa T, Inoue H, Kamachi H. 2005. Augmented ciliary reorientation response and cAMP-dependent protein phosphorylation induced by glycerol in Triton-extracted Paramecium. Zool. Sci. 22: 41-48.
27. Noguchi M, Kurahashi S, Kamachi H, Inoue H. 2004. Control of the ciliary beat by cyclic nucleotides in the intact cortical sheets from Paramecium. Zool. Sci. 21:1167-1175.
28. Noguchi M, Nakamura Y, Okamoto K-I. 1991. Control of ciliary orientation in ciliated sheets from Paramecium-differential distribution of sensitivity to cyclic nucleotides. Cell Motil. Cytoskeleton 20:38-46.
29. Noguchi M, Ogawa T, Taneyama T. 2000. Control of ciliary orientation through cAMP-dependent phosphorylation of axonemal proteins in Paramecium caudatum. Cell Motil. Cytoskeleton 45:263-271.
30. Noguchi M, Sasaki J, Kamachi H, Inoue H. 2003. Protein phosphatase 2C is involved in the cAMP-dependent ciliary control in Paramecium caudatum. Cell Motil. Cytoskeleton 54:95-104.
31. Noguchi M, Sawada T, Akazawa T. 2001. ATP-regenerating system in the cilia of Paramecium caudatum. J. Exp. Biol. 204:1063-1071.
32. Noonan KE, et al. 1990. Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction. Proc. Natl. Acad. Sci. U. S. A. 87:7160-7164.
33. Patel-King RS, King SM. 2009. An outer arm dynein light chain acts in a conformational switch for flagellar motility. J. Cell Biol. 186:283-295.
34. Portzehl H, Caldwell PC, Rüegg JC. 1964. The dependence of contraction and relaxation of muscle fibers from the crab Maia squinado on the internal concentration of free calcium ions. Biochim. Biophys. Acta 79: 581-591.
35. Reynolds ES. 1963. The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol. 17:208-212.
36. Rompolas P, Patel-King RS, King SM. 2010. An outer arm dynein conformational switch is required for metachronal synchrony of motile cilia in planaria. Mol. Biol. Cell 21:3669-3679.
37. Ruiz F, Vayssié L, Klotz C, Sperling L, Madeddu L. 1998. Homologydependent gene silencing in Paramecium. Mol. Biol. Cell 9:931-943.
38. Schultz JE, Klumpp S, Benz R, Schürhoff-Goeters WJ, Schmid A. 1992. Regulation of adenylyl cyclase from Paramecium by an intrinsic potassium conductance. Science 255:600-603.
39. Siebert PD, Larrick JW. 1992. Competitive PCR. Nature 359:557-558.
40. Sonneborn TM. 1970. Methods in Paramecium research, p 241-339. In Prescott D (ed), Methods in cell physiology, vol 4. Academic Press, New York, NY.
41. Sugino K, Naitoh Y. 1982. Simulated cross-bridge patterns corresponding to ciliary beating in Paramecium. Nature 295:609-611.
42. Timmons L, Court DL, Fire A. 2001. Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 263:103-112.
43. Watson ML. 1958. Staining of tissue sections for electron microscopy with heavy metals. J. Biophys. Biochem. Cytol. 4:475-478.
44. Wood CR, Hard R, Hennessey TM. 2007. Targeted gene disruption of dynein heavy chain 7 of Tetrahymena thermophila results in altered ciliary waveform and reduced swim speed. J. Cell Sci. 120:3075-3085.
45. Wu H, et al. 2000. Solution structure of a dynein motor domain associated light chain. Nat. Struct. Biol. 7:575-579.