Gene cloning, expression and activity analysis of Manganese superoxide dismutase from two strains of Gracilaria lemaneiformis (Gracilariaceae, Rhodophyta) under heat stress

Molecules

Volumn 17, Issue 4, 2012, Pages 4522-4532

  Lu, Ning ^a   Zang, Xiaonan ^a   Zhang, Xuecheng ^a   Chen, Hao ^a   Feng, Xiaoting ^a   Zhang, Lu ^a  

^a Ocean University of China   (China)

Author keywords

Gene expression; Gracilaria lemaneiformis; Heat resistance; Mn SOD; SOD activity

Indexed keywords

COMPLEMENTARY DNA; SUPEROXIDE DISMUTASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME ACTIVATION; ENZYMOLOGY; GENETICS; GRACILARIA; HEAT; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ENZYME ACTIVATION; GRACILARIA; HOT TEMPERATURE; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SUPEROXIDE DISMUTASE;

EID: 84860234536     PISSN: None     EISSN: 14203049     Source Type: Journal    
DOI: 10.3390/molecules17044522     Document Type: Article

References (23)

1. Ren, X.Y.; Zhang, X.C. Identification of a putative tetrasporophyte- spectific gene in Gracilaria lemaneiformis (Gracilariaceae, Rhodophyta). J. Ocean Univ. Chin. 2008, 7, 299-303.
2. Fei, X.G. Solving the coastal eutrophication problem by large scale seaweed cultivation. Hydrobiologia 2004, 512, 145-151.
3. Yang, Y.F.; Fei, X.G.; Song, J.M.; Hu, H.Y.; Wang, G.C.; Chung, I.K. Growth of Gracilaria lemaneiformis under different cultivation conditions and its effects on nutrient removal in Chinese coastal waters. Aquaculture 2006, 254, 248-255.
4. Zhou, Y.; Yang, H.S.; Hu, H.Y.; Liu, Y.; Mao, Y.Z.; Zhou, H. Bioremediation potential of the macroalga Gracilaria lemaneiformis (Rhodophyta) integrated into fed fish culture in coastal waters of north China. Aquaculture 2006, 252, 264-276.
5. Brawley, S.H.; Fei, X.G. Ecological studies of Gracilaria asiatica and Gracilaria lemaneiformis in Zhanshan Bay, Qingdao. Chin. J. Oceanol. Limnol. 1988, 6, 20-34.
6. Fei, X.G.; Zhang, X.C.; Chen, W.Z.; Pang, S.J.; Bao, Y. A fast Growing Strain of Gracilaria lemaneiformis KFGL981 and Its Cultivation in China; Kobe International Conference Center: Kobe, Japan, 2007; pp. 26-31.
7. Meng, L.; XU, D.; Chen, W.Z.; Zhang, X.C. Selection and characterization of a new strain of Gracilaria lemaneiformis. J. Ocean Univ. Chin. 2009, 39, S94-S98.
8. Zhang, X.Y.; Hu, C.G.; Yao, J.L. Tetraploidization of diploid Dioscorea results in activation of the antioxidant defense system and increased heat tolerance. J. Plant Physiol. 2010, 167, 88-94.
9. Igor, N.Z.; Thomas, J.M.; Rodney, J.F. Superoxide dismutase gene family: A comparison of the CuZn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression. Free Radic. Biol. Med. 2002, 33, 337-349.
10. Cho, Y.S.; Choi, B.N.; Kin, K.H.; Kim, S.K.; Kim, D.S.; Bang, I.C. Differential expression of Cu/Zn superoxide dismutase mRNA during exposures to heavy metals in rockbream (Oplegnathus fasciatus). Aquaculture 2006, 253, 667-679.
11. Wang, B.Q. The research progress of the plant SOD. J. Hebei Agric. Sci. 2008, 12, 6-9.
12. Leland, S.J.; Andrea, L.W. Long-term hyposaline and hypersaline stresses produce distinct antioxidant responses in the marine alga Dunaliella tertiolecta. J. Plant Physiol. 2003, 160, 1193-1202.
13. Tian, J.Y.; Yu, J. Changes in ultrastructure and responses of antioxidant systems of algae (Dunaliella salina) during acclimation to enhanced ultraviolet-B radiation. J. Photochem. Photobiol. B Biol. 2009, 97, 152-160.
14. Andrzej, B. An enhancing effect of exogenous brassinolide on the growth and antioxidant activity in Chlorella vulgaris cultures under heavy metals stress. Environ. Exp. Bot. 2010, 68, 175-179.
15. Manoj, K.; Puja K.; Vishal, G. Biochemical responses of red alga Gracilaria corticata (Gracilariales, Rhodophyta) to salinity induced oxidative stress. J. Exp. Mar. Biol. Ecol. 2010, 1016, 2-8.
16. Guo, J.J.; Gong, X.G.; Lu, Y.W. Cloning and sequence analyse of a superoxide dismutase gene from Spirulina platensis. J. Zhejiang Univ. (Sci. Ed.) 2004, 31, 674-678.
17. Wang, R.; Liu, T.; Zhou, X.J.; Zhuang, Y.Y.; Mao, Y.X. Cloning and sequence analysing of Mn-SOD Gene from Porphyra yezoensis Udea. Chin. High Technol. Lett. 2006, 16, 522-528.
18. Smith, M.W.; Doolittle R.F. A comparison of evolutionary rates of the two major kinds of superoxide dismutase. J. Mol. Evol. 1992, 34, 175-184.
19. BLAST program. Available online: http://www.ncbi.nlm.nih.gov/BLAST/ (accessed on 22 March 2012).
20. Altschul, S.; Gish, W.; Miller, W.; Myers, E.; Lipman, D. Basic local alignment search tool. J. Mol. Biol. 1990, 215, 403-410.
21. Wan, X.S.; Devalaraja, M.N.; Clair, D. Molecular structure and organization of superoxide dismutase gene. DNA Cell Biol. 1994, 13, 1127-1129.
22. Wei, Z.B.; Miao, X.Y.; Yang, M.Q.; Luo, X.G. Advances in the expression and regulation of Mn-SOD gene. Hereditas 2008, 30, 831-837.
23. Livak, K.J.; Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods 2001, 25, 402-408.