Construction of the highly secreted endochitinase Pichia pastoris strain and the optimization of chitin-degrading conditions

Carbohydrate Polymers

Volumn 89, Issue 1, 2012, Pages 41-45

  Yu, Ping ^a   Tang, Yunping ^a  

^a ZHEJIANG GONGSHANG UNIVERSITY   (China)

Author keywords

Endochitinase; Pichia pastoris; Powder chitin biodegradation; Trichoderma sp.

Indexed keywords

CHITIN DEGRADATION; CHITOOLIGOSACCHARIDES; CULTURE BROTHS; ENDOCHITINASE; PICHIA PASTORIS; RECOMBINANT STRAINS; REDUCING SUGARS; TRICHODERMA SP;

BIODEGRADATION; METHANOL; OPTIMIZATION; SUGARS; YEAST;

CHITIN;

PICHIA PASTORIS; TRICHODERMA;

CHITIN; CHITINASE; COMPLEMENTARY DNA; FUNGAL PROTEIN; RECOMBINANT PROTEIN;

CHEMISTRY; GENETICS; METABOLISM; PH; PICHIA; TEMPERATURE;

CHITIN; CHITINASE; DNA, COMPLEMENTARY; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; PICHIA; RECOMBINANT PROTEINS; TEMPERATURE;

EID: 84860223398     PISSN: 01448617     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.carbpol.2012.02.034     Document Type: Article

References (25)

1. B.B. Aam, E.B. Heggset, A.L. Norberg, M. Sørlie, K.M. Vrum, and V.G.H. Eijsink Production of chitooligosaccharides and their potential applications in medicine Marine Drugs 8 2010 1482 1517
2. S. Anand, and J. Reddy Biocontrol potential of Trichoderma sp. against plant pathogens International Journal of Agriculture Sciences 1 2 2009 30 39
3. I. Aranaz, M. Mengíbar, R. Harris, I. Paños, B. Miralles, and N. Acosta Functional characterization of chitin and chitosan Current Chemical Biology 3 2009 203 230
4. S. Arriaga, J.A. Acosta-Munguía, A.S. Pérez- Martínez, A.D. León-Rodríguez, and A.P. Barba de la Rosa Coupling aerobic biodegradation of methanol vapors with heterologous protein expression of endochitinase Ech42 from Trichoderma atroviride in Pichia pastoris Bioresource Technology 101 2010 9661 9665
5. J.L. Cereghino, and J.M. Cregg Heterologous protein expression in the methylotrophic yeast Pichia pastoris FEMS Microbiology Reviews 24 2000 45 66
6. L. Chen, L. Shen, and J. Wu Expression, purification and in vitro antifungal activity of acidic mammalian chitinase against Candida albicans, Aspergillus fumigatus and Trichophyton rubrum strains Clinical and Experimental Dermatology 34 1 2009 55 60
7. K.V. Harish Prashanth, and R.N. Tharanathan Chitin/chitosan: Modifications and their unlimited application potential - An overview Trends in Food Science and Technology 18 2007 117 131
8. S.J. Horn, P. Sikorski, J.B. Cederkvist, G. Vaaje-Kolstad, M. Sørlie, and B. Synstad Costs and benefits of processivity in enzymatic degradation of recalcitrant polysaccharides Proceeding of the National Academy of Sciences United States of America 143 18 2006 18089 18094
9. R. Jayakumar, M. Prabaharan, S.V. Nair, and H. Tamura Novel chitin and chitosan nanofibers in biomedical applications Biotechnology Advances 28 2010 142 150
10. S. Kudan, and R. Pichyangkura Purification and characterization of thermostable chitinase from Bacillus licheniformis SK-1 Applied Biochemistry and Biotechnology 157 2009 23 35
11. Y. Long, J.Y. Liu, L. Liu, and Z.R. Wang Secretory expression of hirudins in Pichia pastoris Journal of the Fourth Military Medical University 27 8 2006 673 676
12. R.A.A. Muzzarelli Chitin nanostructures in living organisms S. Gupta, Chitin: Formation and diagenesis Vol. 34 2011 Springer Dordrecht 1 34
13. R.A.A. Muzzarelli, J. Boudrant, D. Meyer, N. Manno, M. DeMarchis, and M.G. Paoletti Current views on fungal chitin/chitosan, human chitinases, food preservation, glucans, pectins and inulin: A tribute to Henri Braconnot, precursor of the carbohydrate polymers science, on the chitin bicentennial Carbohydrate Polymers 87 2012 995 1012
14. B. Parameswaran, S. Chandran, and S. Pradeep Fungal biosynthesis of endochitinase and chitobiase in solid state fermentation and their application for the production of N-acetyl-d-glucosamine from colloidal chitin Bioresource Technology 98 2007 2742 2748
15. A.S. Perez-Martinez, A. Deleon-Rodriguez, L.J. Harris, A. Herrera-Estrella, and A.P. Barba de la Rosa Overexpression, purification and characterization of the Trichoderma atroviride endochitinase, Ech42, in Pichia pastoris Protein Expression and Purification 55 2007 183 188
16. L. Ramirez-Coutino, M.D. Marin-Cervantes, S. Huerta, S. Revah, and K. Shirai Enzymatic hydrolysis of chitin in the production of oligosaccharides using Lecanicillium fungicola chitinases Process Biochemistry 41 2006 1106 1110
17. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular cloning. A laboratory manual 2nd ed. 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor
18. Y. Tao, H. Jin, Z.F. Long, L. Zhang, X.Q. Ding, and K. Tao Cloning and expression of a chitinase gene from Sanguibacter sp. C4 Yi Chuan Xue Bao 33 2006 1037 1046
19. Wu, M. B. (2008). Studies on the production processes, kinetics, bioreactor and properties of endochitinase and chitosanase with Trichoderma viride. PhD thesis, Zhejiang University, China.
20. M. Yamabhai, S. Emrat, S. Sukasem, P. Pesatcha, N. Jaruseranee, and B. Buranabanyat Secretion of recombinant Bacillus hydrolytic enzymes using Escherichia coli expression systems Journal of Biotechnology 133 2008 50 57
21. P. Yu, and J.R. Li Studies on the production of chitinase by fermentation using Trichoderma viride HZ012 Journal of Chinese Institute of Food Science and Technology 6 2006 26 30
22. P. Yu, and J.R. Li Molecular cloning, sequence analysis, expression and characterization of the endochitinase gene from Trichoderma sp. in Escherichia coli BL21 World Journal of Microbiology and Biotechnology 24 2008 2509 2516
23. L.X. Zhang, T.F. Zhang, and L.Y. Li Method and technology of biochemistry 2nd ed. 1997 Higher Education Press. Beijing
24. Y.F. Zhou, X.E. Zhang, and E.G.C. Anthony Advances in enzyme molecular cloning and expression Chinese Journal of Biotechnology 18 4 2008 401 406
25. W. Zhang, W. Zhang, Q.X. Jiang, and W.S. Xia Physicochemical and structural characteristics of chitosan nanopowders prepared by ultrafine milling Carbohydrate Polymers 87 2012 309 313