Protein analysis as a measure of active biomass in activated sludge

Water Science and Technology

Volumn 65, Issue 8, 2012, Pages 1483-1489

  Yucesoy E ^a   Ludemann N ^a   Lucas, H ^a   Tan, J ^a   Denecke, M ^a  

^a UNIVERSITY OF DUISBURG ESSEN   (Germany)

Author keywords

Activated sludge; Active biomass; Protein; Starvation

Indexed keywords

ACTIVATED SLUDGE; ACTIVE BIOMASS; BIOMASS CONCENTRATIONS; CONVENTIONAL METHODS; ORGANIC MATERIALS; OXYGEN UPTAKE RATES; PROTEIN ANALYSIS; PROTEIN CONTENTS; PROTEIN FRACTIONS; STARVATION; STARVATION CONDITIONS; TOTAL SUSPENDED SOLIDS; VOLATILE SUSPENDED SOLIDS;

BIOMASS; PROTEINS;

BIOACTIVITY;

VOLATILE AGENT;

ACTIVATED SLUDGE; BIOACTIVITY; BIOCHEMICAL COMPOSITION; BIOMASS; CONCENTRATION (COMPOSITION); EXPERIMENTAL STUDY; OXYGEN CONSUMPTION; PROTEIN; STARVATION; SUSPENDED LOAD; VOLATILE SUBSTANCE;

ACTIVATED SLUDGE; ARTICLE; BIOLOGICAL ACTIVITY; BIOMASS; CONTROLLED STUDY; NONHUMAN; OXYGEN CONSUMPTION; PROTEIN ANALYSIS; PROTEIN CONTENT; STARVATION; SUSPENDED PARTICULATE MATTER;

BIOMASS; BIOREACTORS; CARBON; OXYGEN; PROTEINS; SEWAGE; STARVATION;

EID: 84860122005     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.2166/wst.2012.029     Document Type: Article

References (27)

1. Ali, I., Khararjian, H. & Ahmed, M. 1985 Viability of microbial mass in compartmentalised single activated sludge process. Water Research 19 (7), 927-932. (Pubitemid 15065300)
2. Atlas, R. & Bartha, R. 1987 Microbial Ecology: Fundamentals and Applications, 2nd edition. The Benjamin Cummings Publishing Co Inc., Menlo Park.
3. Beck, D. J. & Bibby, B. G. 1961 A modified anthrone colorimetric technique for use in investigations related to the cariogenicity of foodstuffs. Journal of Dental Research 40, 161.
4. Denecke, M. 2006 Protein extraction from activated sludge. Water Science and Technology 54 (1), 175-181. (Pubitemid 44150731)
5. DIN EN 12789 Characterization of sludges - Determination of the loss on ignition of dry mass.
6. DIN EN 12880 Characterization of sludges - Determination of dry residue and water content.
7. DIN NORM 38412 German standard methods for the examination of water, waste water and sludge Bio assays.
8. Droste, R. 1997 Theory and Practice of Water and Wastewater Treatment. John Wiley and Sons, New York.
9. Durmaz, B. & Sanin, F. 2001 Effect of carbon to nitrogen ratio on the composition of microbial extracellular polymers in activated sludge. Water Science and Technology 10 (22), 1-9.
10. Ehlers, M. M. & Cloete, T. E. 1999 Direct extraction of proteins to monitor an activated sludge system on a weekly basis for 34 weeks using SDS-page. Water SA 25 (1), 57-62. (Pubitemid 29073209)
11. Frølund, B., Palmgren, R., Keiding, K. & Nielsen, P. H. 1995 Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Research 30 (8), 1749-1758.
12. Gaudy Jr, A. & Gaudy, E. 1980 Microbiology for Environmental Scientists and Engineers. McGraw Hill, New York.
13. Henze, M., Grady, C., Gujer, W., Marais, G. & Matsuo, T. 1987 A general model for single-sludge wastewater treatment systems. Water Research 21 (5), 505-515.
14. Hwang, S. & Hansen, C. 1998 Evaluating a correlation between volatile suspended solid and adenosine 50-triphosphate levels in anaerobic treatment of high organic suspended solids wastewater. Bioresource Technology 63 (3), 243-250. (Pubitemid 28181588)
15. Kirchman, D. 2001 Measuring bacterial biomass production and growth rates from leucine incorporation in natural aquatic environments. Methods in Microbiology. Marine Microbiology 30, 227-237. (Pubitemid 33805051)
16. McComis, W. & Litchfield, J. 1989 Meat, fish, and poultry processing wastes. Journal of the WPCF 61 (6), 855-865.
17. Pollard, P., Münch, E., Lant, P. & Greenfield, P. 1997 The impact of microbiological tools on mathematical modelling of biological wastewater treatment. Water Science and Technology 36 (10), 97-108. (Pubitemid 28025183)
18. Robarts, R. & Zohary, T. 1993 Fact or fiction - Bacterial growth rates and production as determined by [methyl-H]- thymidine? In: Advances in Microbial Ecology (G. Jones, ed.). Plenum Press, New York, pp. 371-425.
19. Robertson, B., Button, D. & Koch, A. 1998 Determination of the biomasses of small bacteria at low concentrations in a mixture of species with forward light scatter measurements by flow cytometry. Applied Environmental Microbiology 64 (10), 3900-3909. (Pubitemid 28462990)
20. Schmitz, U., Berger, C. R. & Orth, H. 2000 Protein analysis as a simple method for the quantitative assessment of sewage sludge disintegration. Water Research 34 (14), 3682-3685. (Pubitemid 30488140)
21. Sherr, B., Sherr, E. & del Giorgio, P. 2001 Enumeration of total and highly active bacteria. Methods in Microbiology. Marine Microbiology 30, 129-159. (Pubitemid 33805048)
22. Standard Methods for the Examination of Water andWastewater, 1998 A. E. Greenberg, L. S. E. Clesceri & A. D. Eaton (eds). American Public Health Association, Washington D. C., USA.
23. Sutton, S. 2002 Quantification of microbial biomass. In: Encyclopedia of Environmental Microbiology (G. Bitton, ed.). John Wiley & Sons, New York, USA, pp. 2652-2660.
24. Tchobanoglous, G., Burton, F. L. & Stensel, H. D. 2003 Wastewater Engineering Treatment and Reuse. McGraw-Hill, California.
25. Urbain, V., Block, J. C. & Manem, J. 1993 Bioflocculation in activated sludge: analytical approach. Water Research 27 (5), 829-838.
26. Wang, Z.-W., Li, Y., Zhou, J.-Q. & Liu, Y. 2006 The influence of short-term starvation on aerobic granules. Process Biochemistry 41 (12), 2373-2378. (Pubitemid 44602556)
27. Wilmes, O. & Bond, P. L. 2004 The application of two dimensional polyacrylamide gel electrophoresis and downstream analyses to a mixed community of prokaryotic microorganisms. Environmental Microbiology 6 (9), 911-920. (Pubitemid 39123245)