Crystal structure of a dimeric Ser49 PLA 2-like myotoxic component of the Vipera ammodytes meridionalis venomics reveals determinants of myotoxicity and membrane damaging activity

Molecular BioSystems

Volumn 8, Issue 5, 2012, Pages 1405-1411

  Georgieva, Dessislava ^a,b   Coronado, Monika ^a   Oberthür, Dominik ^a   Buck, Friedrich ^c   Duhalov, Deyan ^d   Arni, Raghuvir K ^e   Betzel, Christian ^a,e  

^a UNIVERSITY OF HAMBURG   (Germany)

^b INSTITUTE OF ORGANIC CHEMISTRY   (Bulgaria)

^c UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^d NATIONAL CENTER OF INFECTIOUS AND PARASITIC DISEASES   (Bulgaria)

^e SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; PHOSPHOLIPASE A2; SERINE; VIPER VENOM;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CELL MEMBRANE; CHEMISTRY; DRUG EFFECT; ENZYME ACTIVE SITE; HYDROGEN BOND; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PROTEIN MULTIMERIZATION; PROTEIN SECONDARY STRUCTURE; PROTEOMICS; SEQUENCE HOMOLOGY; VIPERIDAE; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ACID; CATALYTIC DOMAIN; CELL MEMBRANE; CRYSTALLOGRAPHY, X-RAY; HYDROGEN BONDING; MOLECULAR SEQUENCE DATA; PHOSPHOLIPASES A2; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, SECONDARY; PROTEOMICS; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; VIPER VENOMS; VIPERIDAE;

VIPERA AMMODYTES MERIDIONALIS; VIPERINAE;

EID: 84859850974     PISSN: 1742206X     EISSN: 17422051     Source Type: Journal    
DOI: 10.1039/c2mb05490f     Document Type: Article

References (42)

1. M. Ohno T. Chijiwa N. Oda-Ueda T. Ogawa S. Hattori Molecular evolution of myotoxic phospholipases A2 from snake venom Toxicon 2003 42 841 854
2. 2 homologue from Vipera ammodytes venom Eur. J. Biochem. 1991 202 1165 1168
3. 2 J. Mol. Biol. 1990 216 425 439
4. 2 myotoxins from Bothrops snake venoms Toxicon 1995 33 1405 1424
5. 2 proteins Toxicon 1999 37 411 445
6. R. M. Kini H. J. Evans Role of cationic residues in cytolytic activity: modification of lysine residues in the cardiotoxin from Naja nigricollis venom and correlation between cytolytic and antiplatelet activity Biochemistry 1989 28 9209 9216
7. A. M. Soares J. R. Giglio Chemical modifications of phospholipases A2 from snake venoms: effects on catalytic and pharmacological properties Toxicon 2003 42 855 868
8. 2 from Bothrops asper snake venom. Identification of a heparin-binding and cytolytic toxin region by the use of synthetic peptides and molecular modelling J. Biol. Chem. 1994 269 29867 29873
9. 2 from Agkistrodon piscivorus piscivorus snake venom: synthetic C-terminal peptides from Lys49, but not from Asp49 myotoxins, exert membrane-damaging activities Toxicon 2001 39 1587 1594
10. 2 homologue Int. J. Biochem. Cell Biol. 2002 34 1268 1278
11. 2 from the venom of the cottonmouth snake at 2.0 Å resolution J. Biol. Chem. 1990 265 17649 17656
12. 2 from the venom of Agkistrodon piscivorus piscivorus J. Biol. Chem. 1992 267 22645 22657
13. M. T. Murakami E. Z. Arruda P. A. Melo A. B. Martinez S. Calil-Eliás M. A. Tomaz B. Lomonte J. M. Gutiérrez R. K. Arni Inhibition of myotoxic activity of Bothrops asper myotoxin II by the anti-trypanosomal drug suramin J. Mol. Biol. 2005 350 416 426
14. 2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid Biochimie 2005 87 161 167
15. 2 homologue from Atropoides nummifer venom Acta Crystallogr., Sect. F: Struct. Biol. Cryst. Commun. 2006 62 423 426
16. 2 homologues Toxicon 2007 49 378 387
17. 2 activity based on ligand-induced conformational change J. Biol. Chem. 2005 280 7326 7335
18. J. Pungerar N. Liang B. Štrukelj F. GubenŠek Nucleotide sequence of a cDNA encoding ammodytin L Nucleic Acids Res. 1990 18 4601
19. S. Rufini P. Cesaroni A. Desideri R. Farias F. Gubensek J. M. Gutiérrez P. Luly R. Massoud R. Morero J. Z. Pedersen Calcium ion independent membrane leakage induced by phospholipase-like myotoxins Biochemistry 1992 31 12424 12430
20. J. Z. Pedersen B. Lomonte R. Massoud F. Gubensek J. M. Gutiérrez S. Rufini Autocatalytic acylation of phospholipase-like myotoxins Biochemistry 1995 34 4670 4675
21. S. Incerpi P. de Vito P. Luly S. Rufini Effect of ammodytin L from Vipera ammodytes on L-6 cells from rat skeletal muscle Biochim. Biophys. Acta 1995 1268 137 142
22. T. Petan I. Krizaj J. Pungercar Restoration of enzymatic activity in a Ser-49 phospholipase A2 homologue decreases its Ca(2+)-independent membrane-damaging activity and increases its toxicity Biochemistry 2007 46 12795 12809
23. 2, ecarpholin S, from the venom of Echis carinatus sochureki (saw-scaled viper) Biochem. J. 1996 319 961 968
24. X. Zhou T.-C. Tan S. Valiyaveettil M. L. Go R. M. Kini A. Velazquez-Campoy J. Sivaraman Structural Characterization of Myotoxic Ecarpholin S from Echis carinatus Venom Biophys. J. 2008 95 3366 3380
25. R. K. Arni R. J. Ward Phospholipase A2 - a structural review Toxicon 1996 34 827 841
26. M. T. Murakami R. K. Arni A structure based model for liposome disruption and the role of catalytic activity in myotoxic phospholipase A2s Toxicon 2003 42 903 913
27. R. Renetseder S. Brunie B. W. Dijkstra J. Drenth P. Sigler A comparison of the crystal structures of phospholipase A2 from bovine pancreas and Crotalus atrox venom J. Biol. Chem. 1985 260 11627 11634
28. D. L. Scott S. P. White Z. Otwiniwski W. Yuan M. H. Gelb P. B. Sigler Interfacial catalysis: the mechanism of phospholipase A2 Science 1990 250 1541 1546
29. 2+ free and bound states Biochimie 2006 88 543 549
30. 2 complexed with a substrate-derived inhibitor Nature 1990 347 689 691
31. W.-H. Lee M. T. da Silva Giotto S. Marangoni M. H. Toyama I. P. C. Garratt Structural basis for low catalytic activity in Lys49 phospholipases A2. A hypothesis: the crystal structure of piratoxin II complexed to fatty acid Biochemistry 2001 40 28 36
32. S. Banumathi K. R. Rajashankar C. Nötzel B. Aleksiev T. P. Singh N. Genov Ch. Betzel Structure of the neurotoxic complex vipoxin at 1.4 Å resolution Acta Crystallogr., Sect. D: Biol. Crystallogr. 2001 57 1552 1559
33. 2 from the venom of Bulgarian viper (Vipera ammodytes meridionalis) Toxicon 1976 14 477 485
34. 2 activity-independent mechanism of action of β-neurotoxins in fish? Biochem. Biophys. Res. Commun. 1998 244 514 518
35. D. Georgieva M. Risch A. Kardas F. Buck M. von Bergen Ch. Betzel Comparative analysis of the venom proteomes of Vipera ammodytes ammodytes and Vipera ammodytes meridionalis J. Proteome Res. 2008 7 866 886
36. C. R. Cantor and P. R. Schimmel, Size and shape of macromolecules, Biophysical Chemistry, Part II, W. H. Freeman and Company, New York, 1980, pp. 539-590
37. Z. Otwinowski W. Minor Processing of X-ray diffraction data collected in oscillation mode Methods Enzymol. 1996 276 307 326
38. A. J. McCoy R. W. Grosse-Kunstleve P. D. Adams M. D. Winn L. C. Storoni R. J. Read Phaser crystallographic software J. Appl. Crystallogr. 2007 40 658 674
39. P. D. Adams P. V. Afonine G. Bunkóczi V. B. Chen I. W. Davis N. Echols J. J. Headd L.-W. Hung G. J. Kapral R. W. Grosse-Kunstleve A. J. McCoy N. W. Moriarty R. Oeffner R. J. Read D. C. Richardson J. S. Richardson T. C. Terwilliger P. H. Zwart A comprehensive Python-based system for macromolecular structure solution Acta Crystallogr., Sect. D: Biol. Crystallogr. 2010 66 213 221
40. Collaborative Computational Project, Number 4, Acta Crystallogr., Sect. D: Biol. Crystallogr., 1994, 50, 760 763
41. P. Emsley K. Cowtan Coot: model-building tools for molecular graphics Acta Crystallogr., Sect. D: Biol. Crystallogr. 2004 60 2126 2132
42. R. A. Laskowski M. W. MacArthur D. S. Moss J. M. Thornton Procheck: a program to check stereochemical quality of protein structures J. Appl. Crystallogr. 1993 26 183 291