Purification and characterization of an organic solvent-stable lipase from Pseudomonas stutzeri LC2-8 and its application for efficient resolution of (R, S)-1-phenylethanol

Biochemical Engineering Journal

Volumn 64, Issue , 2012, Pages 55-60

  Cao, Yan ^a   Zhuang, Yu ^a   Yao, Changjin ^a   Wu, Bin ^a   He, Bingfang ^a  

^a NANJING TECH UNIVERSITY   (China)

Author keywords

Biocatalysis; Enantioseparation; Lipase; Organic solvent stability; Pseudomonas stutzeri; Purification

Indexed keywords

AMINO ACID RESIDUES; ANION EXCHANGE CHROMATOGRAPHY; BIOCATALYSIS; ENANTIOMERIC EXCESS; ENANTIOSEPARATIONS; HYDROLYTIC ACTIVITIES; HYDROPHILIC SOLVENTS; ISO-PROPANOLS; LIPASE ACTIVITY; NON-AQUEOUS SYSTEM; OPEN READING FRAME; OPTIMUM PH; ORGANIC SOLVENT-STABILITY; PHENYLETHANOL; PSEUDOMONAS STUTZERI; SDS-PAGE;

ACETONE; AMINO ACIDS; BACTERIA; LIPASES; METHANOL; ORGANIC SOLVENTS; PURIFICATION; SOLVENTS;

ETHANOL;

1 PHENYLETHANOL; 4 NITROPHENYL HEXANOATE; ACETONE; AMINO ACID; HEXANOIC ACID; ORGANIC SOLVENT; SURFACTANT; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; ENANTIOMER; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; HALF LIFE TIME; HYDROLYSIS; HYDROPHILICITY; MOLECULAR CLONING; MOLECULAR WEIGHT; OPEN READING FRAME; PH; PRECIPITATION; PRIORITY JOURNAL; PSEUDOMONAS STUTZERI; SEQUENCE HOMOLOGY; TEMPERATURE; TRANSESTERIFICATION;

BACTERIA (MICROORGANISMS); PSEUDOMONAS STUTZERI;

EID: 84859846840     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2012.03.004     Document Type: Article

References (19)

1. Baharum S.N., Salleh A.B., Razak C.N.A., Basri M., Rahman M.B.A., Rahman R. Organic solvent tolerant lipase by Pseudomonas sp strain S5: stability of enzyme in organic solvent and physical factors affecting its production. Ann. Microbiol. 2003, 53:75-83.
2. Klibanov A.M. Enzymes that work in organic solvents. Chem. Tech. 1986, 16:354-359.
3. Ogino H., Miyamoto K., Ishikawa H. Organic-solvent-tolerant bacterium which secretes organic-solvent-stable lipolytic enzyme. Appl. Environ. Microbiol. 1994, 60:3884-3886.
4. Hun C.J., Rahman R., Salleh A.B., Basri M. A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase. Biochem. Eng. J. 2003, 15:147-151.
5. Zhao L.L., Xu J.H., Zhao J., Pan J., Wang Z.L. Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010. Process Biochem. 2008, 43:626-633.
6. Winkler U.K., Stuckmann M. Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by serratia-marcescens. J. Bacteriol. 1979, 138:663-670.
7. Bradford M.M. Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
8. Laemmli U.K. Cleavage of structural proteins during assembly of head of bacteriophage-T4. Nature 1970, 227:680-685.
9. Perkins D.N., Pappin D.J.C., Creasy D.M., Cottrell J.S. Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 1999, 20:3551-3567.
10. Yan Y.L., Yang J., Dou Y.T., Chen M., Ping S.Z., Peng J.P., Lu W., Zhang W., Yao Z.Y., Li H.Q., Liu W., He S., Geng L.Z., Zhang X.B., Yang F., Yu H.Y., Zhan Y.H., Li D.H., Lin Z.L., Wang Y.P., Elmerich C., Lin M., Jin Q. Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated Pseudomonas stutzeri A1501. Proc. Natl. Acad. U. S. A. 2008, 105:7564-7569.
11. Ogino H., Nakagawa S., Shinya K., Muto T., Fujimura N., Yasuda M., Ishikawa H. Purification and characterization of organic solvent-stable lipase from organic solvent-tolerant Pseudomonas aeruginosa LST-03. J. Biosci. Bioeng. 2000, 89:451-457.
12. Doukyu N., Ogino H. Organic solvent-tolerant enzymes. Biochem. Eng. J. 2010, 48:270-282.
13. Ogino H., Ishikawa H. Enzymes which are stable in the presence of organic solvents. J. Biosci. Bioeng. 2001, 91:109-116.
14. Zhang A.J., Gao R.J., Diao N.B., Xie G.Q., Gao G., Cao S.G. Cloning, expression and characterization of an organic solvent tolerant lipase from Pseudomonas fluorescens JCM5963. J. Mol. Catal. B: Enzym. 2009, 56:78-84.
15. Wang X.Q., Yu X.W., Xu Y. Homologous expression, purification and characterization of a novel high-alkaline and thermal stable lipase from Burkholderia cepacia ATCC 25416. Enzyme Microb. Technol. 2009, 45:94-102.
16. Colton I.J., Ahmed S.N., Kazlauskas R.J. A 2-propanol treatment increases the enantioselectivity of Candida rugosa lipase toward esters of chiral carboxylic acids. J. Org. Chem. 1995, 60:212-217.
17. Long Z.D., Xu J.H., Zhao L.L., Pan J., Yang S., Hua L. Overexpression of Serratia marcescens lipase in Escherichia coli for efficient bioresolution of racemic ketoprofen. J. Mol. Catal. B: Enzym. 2007, 47:105-110.
18. Habulin, Maja, Knez, Zeljko Optimization of (R, S)-l-phenylethanol kinetic resolution over Candida antarctica lipase B in ionic liquids. J. Mol. Catal. B: Enzym. 2009, 58:24-28.
19. Xue P., Yan X.H., Wang Z. Lipase immobilized on HOOC-MCF: a highly enantioselective catalyst for transesterification resolution of (R, S)-1-phenylethanol. Chin. Chem. Lett. 2007, 18:929-932.