Exon skipping quantification by real-time PCR

Methods in Molecular Biology

Volumn 867, Issue , 2012, Pages 189-199

  Ferlini, Alessandra ^a   Rimessi, Paola ^a  

^a UNIVERSITY OF FERRARA   (Italy)

Author keywords

Assay; Exon skipping; Fluorogenic; Probe; RT PCR; TaqMan

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; DYSTROPHIN; FLUORESCENT DYE;

ANIMAL; ARTICLE; DUCHENNE MUSCULAR DYSTROPHY; EXON; GENETICS; HUMAN; METHODOLOGY; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; DYSTROPHIN; EXONS; FLUORESCENT DYES; HUMANS; MUSCULAR DYSTROPHY, DUCHENNE; OLIGONUCLEOTIDES, ANTISENSE; REAL-TIME POLYMERASE CHAIN REACTION;

ANIMALIA;

EID: 84859808073     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-767-5_12     Document Type: Article

References (7)

1. Thörn I, Botling J, Hermansson M, Lönnerholm G, Sundström C, Rosenquist R et al (2009) Monitoring minimal residual disease with fl ow cytometry, antigen-receptor gene rearrangements and fusion transcript quantification in Philadelphia-positive childhood acute lymphoblastic leukemia. Leuk Res 33:1047-1054
2. Sellars MJ, Vuocolo T, Leeton LA, Coman GJ, Degnan BM, Preston NP (2007) Real-time RT-PCR quantification of Kuruma shrimp transcripts: a comparison of relative and absolute quantification procedures. J Biotechnol 129: 391-399 (Pubitemid 46562491)
3. Gutiérrez MI, Timson G, Siraj AK, Bu R, Barbhaya S, Banavali S et al (2005) Single monochrome real-time RT-PCR assay for identification, quantification, and breakpoint cluster region determination of t(9;22) transcripts. J Mol Diagn 7:40-47 (Pubitemid 40310164)
4. Altarescu G, Eldar-Geva T, Varshower I, Brooks B, Haran EZ, Margalioth EJ et al (2009) Real-time reverse linkage using polar body analysis for preimplantation genetic diagnosis in female carriers of de novo mutations. Hum Reprod 24:3225-3229
5. Spitali P, Rimessi P, Fabris M, Perrone D, Falzarano S, Bovolenta M et al (2009) Exon skipping-mediated dystrophin reading frame restoration for small mutations. Hum Mutat 30:1527-1534
6. Ferlini A, Sabatelli P, Fabris M, Bassi E, Falzarano S, Vattemi G et al (2010) Dystrophin restoration in skeletal, heart and skin arrector pili smooth muscle of mdx mice by ZM2 NP-AON complexes. Gene Ther 17: 432-438
7. Rimessi P, Sabatelli P, Fabris M, Braghetta P, Bassi E, Spitali P et al (2009) Cationic PMMA nanoparticles bind and deliver antisense oligoribonucleotides allowing restoration of dystrophin expression in the mdx mouse. Mol Ther 88:17-24,