De novo biosynthesis of glycosaminoglycans in the extracellular matrix of skin studied by matrix-assisted laser desorption/ionization mass spectrometry

Analytical Biochemistry

Volumn 421, Issue 2, 2012, Pages 791-793

  Böhme, Julia ^a   Anderegg, Ulf ^a   Nimptsch, Ariane ^a   Nimptsch, Kathrin ^a   Hacker, Michael ^a   Schulz Siegmund, Michaela ^a   Huster, Daniel ^a   Schiller, Jürgen ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Biosynthesis; Dermatan sulfate; Glycosaminoglycans; MALDI TOF MS; Skin

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; CELL CULTURE; DESORPTION; FIBROBLASTS; INDUCTIVELY COUPLED PLASMA; SKIN; SULFUR COMPOUNDS;

DERMATAN SULFATE; EXTRACELLULAR MATRICES; GLYCOSAMINOGLYCANS; MALDI TOF MS; MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY; MATRIX-ASSISTED LASER DESORPTION/IONIZATION-TIME-OF-FLIGHT MASS SPECTROMETRY; MOLECULAR COMPOSITIONS; TISSUE ENGINEERED PRODUCTS;

MASS SPECTROMETRY;

CARBON 13; DERMATAN SULFATE; GLUCOSE; GLYCOSAMINOGLYCAN;

ANALYTIC METHOD; ARTICLE; BIOSYNTHESIS; CHEMICAL STRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; EX VIVO STUDY; EXTRACELLULAR MATRIX; FIBROBLAST CULTURE; HUMAN; HUMAN CELL; ISOTOPE LABELING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; QUALITY CONTROL; SKIN; TISSUE ENGINEERING;

EID: 84859717439     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.11.002     Document Type: Article

References (12)

1. J. Mansbridge, Skin tissue engineering, J. Biomater. Sci. Polym. Ed. 19 (2008) 955-968. (Pubitemid 351957384)
2. M.H. Flint, A.S. Craig, H.C. Reilly, G.C. Gillard, D.A. Parry, Collagen fibril diameters and glycosaminoglycan content of skins: Indices of tissue maturity and function, Connect. Tissue Res. 13 (1984) 69-81. (Pubitemid 14032009)
3. J. Schiller, B. Fuchs, K. Arnold, The molecular organization of polymers of cartilage: an overview of health and disease, Curr. Org. Chem. 10 (2006) 1771-1789. (Pubitemid 44355993)
4. E. Sisu, C. Flangea, A. Serb, A.D. Zamfir, Modern developments in mass spectrometry of chondroitin and dermatan sulfate glycosaminoglycans, Amino Acids 41 (2011) 235-256.
5. F. Hillenkamp, J. Peter-Katalinić,MALDI MS: a practical guide to instrumentation, methods, and application, Wiley-VCH, Weinheim, Germany, 2007.
6. A. Nimptsch, S. Schibur, M. Schnabelrauch, B. Fuchs, D. Huster, J. Schiller, Characterization of the quantitative relationship between signal-to-noise (S/N) ratio and sample amount on-target by MALDI-TOF MS: determination of chondroitin sulfate subsequent to enzymatic digestion, Anal. Chim. Acta 635 (2009) 175-182.
7. R. Schulz, S. Höhle, G. Zernia, M. Zscharnack, J. Schiller, A. Bader, K. Arnold, D. Huster, Analysis of extracellular matrix production in artificial cartilage constructs by histology, immunocytochemistry, mass spectrometry, and NMR spectroscopy, J. Nanosci. Nanotechnol. 6 (2006) 2368-2381. (Pubitemid 44599061)
8. M. Hacker, M. Ringhofer, B. Appel, M. Neubauer, T. Vogel, S. Young, A.G. Mikos, T. Blunk, A. Göpferich, M.B. Schulz, Solid lipid templating of macroporous tissue engineering scaffolds, Biomaterials 28 (2007) 3497-3507. (Pubitemid 46817652)
9. J. Schiller, J. Arnhold, S. Benard, S. Reichl, K. Arnold, Cartilage degradation by hyaluronate lyase and chondroitin ABC lyase: a MALDI-TOF mass spectrometric study, Carbohydr. Res. 318 (1999) 116-122. (Pubitemid 29421790)
10. M. Zscharnack, P. Hepp, R. Richter, T. Aigner, R. Schulz, J. Somerson, C. Josten, A. Bader, B. Marquass, Repair of chronic osteochondral defects using predifferentiated mesenchymal stem cells in an ovine model, Am. J. Sports Med. 38 (2010) 1857-1869.
11. J. Zaia, C.E. Costello, Compositional analysis of glycosaminoglycans by electrospray mass spectrometry, Anal. Chem. 73 (2001) 233-239. (Pubitemid 32061834)
12. A. Nimptsch, S. Schibur, C. Ihling, A. Sinz, T. Riemer, D. Huster, J. Schiller, Quantitative analysis of denatured collagen by collagenase digestion and subsequent MALDI-TOF mass spectrometry, Cell Tissue Res. 343 (2011) 605-617.