A modified method for the purification of active large enzymes using the glutathione S-transferase expression system

Analytical Biochemistry

Volumn 421, Issue 2, 2012, Pages 805-807

  Deceglie, Stefania ^a   Lionetti, Claudia ^a   Roberti, Marina ^a   Cantatore, Palmiro ^a   Loguercio Polosa, Paola ^a  

^a UNIVERSITY OF BARI   (Italy)

Author keywords

GST fusion protein; Mitochondrial RNA polymerase; Protein affinity purification

Indexed keywords

ENZYMES; MITOCHONDRIA; PEPTIDES; PURIFICATION; RNA; SOAPS (DETERGENTS);

EXPRESSION SYSTEM; FUNCTIONAL ACTIVITIES; FUSION PROTEINS; GLUTATHIONE S-TRANSFERASES; HIGH LEVEL EXPRESSION; PROTEIN AFFINITY; RNA POLYMERASE; TAGGED PROTEINS;

RECOMBINANT PROTEINS;

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; RNA POLYMERASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME PURIFICATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION;

EID: 84859705667     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.12.015     Document Type: Article

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