Folate deficiency is associated with the formation of complex nuclear anomalies in the cytokinesis-block micronucleus cytome assay

Environmental and Molecular Mutagenesis

Volumn 53, Issue 4, 2012, Pages 311-323

  Bull, Caroline F ^a,b   Mayrhofer, Graham ^b   Zeegers, Dimphy ^c   Mun, Grace Low Kah ^c   Hande, M Prakash ^c,d   Fenech, Michael F ^a  

^a CSIRO   (Australia)

^b UNIVERSITY OF ADELAIDE   (Australia)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

CBMN Cyt assay; DNA damage; FISH; Folate; Human lymphocytes; Mitosis; Nutrition

Indexed keywords

NUCLEIC ACID;

ARTICLE; CENTROMERE; CHROMOSOMAL INSTABILITY; CHROMOSOME; CHROMOSOME SEGREGATION; CYTOKINESIS; DISEASE ASSOCIATION; FLUORESCENCE IN SITU HYBRIDIZATION; FOLIC ACID DEFICIENCY; HUMAN; HUMAN CELL; METAPHASE; MICRONUCLEUS; MICRONUCLEUS TEST; MITOSIS; SISTER CHROMATID; TELOMERE;

ADULT; ANALYSIS OF VARIANCE; BIOLOGICAL MARKERS; CELL NUCLEUS; CHROMOSOMAL INSTABILITY; CYTOGENETIC ANALYSIS; FOLIC ACID DEFICIENCY; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; LYMPHOCYTES; MALE; MICRONUCLEUS TESTS; PEPTIDE NUCLEIC ACIDS;

EQUIDAE;

EID: 84859596475     PISSN: 08936692     EISSN: 10982280     Source Type: Journal    
DOI: 10.1002/em.21688     Document Type: Article

References (31)

1. Beetstra S, Thomas P, Salisbury C, Turner J, Fenech M. 2005. Folic acid deficiency increases chromosomal instability, chromosome 21 aneuploidy and sensitivity to radiation-induced micronuclei. Mutat Res 578: 317-326.
2. Blasco MA. 2007. The epigenetic regulation of mammalian telomeres. Nat Rev Genet 8: 299-309.
3. Boos G, Stopper H. 2001. DNA methylation influences the decatenation activity of topoisomerase II. Int J Biol Macromol 28: 103-106.
4. Bull C, Fenech M. 2008. Genome-health nutrigenomics and nutrigenetics: Nutritional requirements or 'nutriomes' for chromosomal stability and telomere maintenance at the individual level. Proc Nutr Soc 67: 146-156.
5. Bull CF, Beetstra-Hill S, Benassi-Evans BJ, Crott JW, Kimura M, Teo T, Wu J, Fenech MF. 2011. Application and adaptation of the in vitro micronucleus assay for the assessment of nutritional requirements of cells for DNA damage prevention. Mutagenesis 26: 193-197.
6. Chan KL, Palmai-Pallag T, Ying S, Hickson ID. 2009. Replication stress induces sister-chromatid bridging at fragile site loci in mitosis. Nat Cell Biol 11: 753-760.
7. Coelho PA, Queiroz-Machado J, Sunkel CE. 2003. Condensin-dependent localisation of topoisomerase II to an axial chromosomal structure is required for sister chromatid resolution during mitosis. J Cell Sci 116: 4763-4776.
8. Crott JW, Mashiyama ST, Ames BN, Fenech M. 2001. The effect of folic acid deficiency and MTHFR C677T polymorphism on chromosome damage in human lymphocytes in vitro. Cancer Epidemiol Biomarkers Prev 10: 1089-1096.
9. Decordier I, Cundari E, Kirsch-Volders M. 2008. Mitotic checkpoints and the maintenance of the chromosome karyotype. Mutat Res 651: 3-13.
10. Duthie SJ, Hawdon A. 1998. DNA instability (strand breakage, uracil misincorporation, and defective repair) is increased by folic acid depletion in human lymphocytes in vitro. FASEB J 12: 1491-1497.
11. Fenech M. 2001. The role of folic acid and Vitamin B12 in genomic stability of human cells. Mutat Res 475: 57-67.
12. Fenech M. 2007. Cytokinesis-block micronucleus cytome assay. Nat Protoc 2: 1084-1104.
13. Fenech M, Crott JW. 2002. Micronuclei, nucleoplasmic bridges and nuclear buds induced in folic acid deficient human lymphocytes-evidence for breakage-fusion-bridge cycles in the cytokinesis-block micronucleus assay. Mutat Res 504: 131-136.
14. Fenech M, Baghurst P, Luderer W, Turner J, Record S, Ceppi M, Bonassi S. 2005. Low intake of calcium, folate, nicotinic acid, vitamin E, retinol, beta-carotene and high intake of pantothenic acid, biotin and riboflavin are significantly associated with increased genome instability: Results from a dietary intake and micronucleus index survey in South Australia. Carcinogenesis 26: 991-999.
15. Fenech M, Kirsch-Volders M, Natarajan AT, Surralles J, Crott JW, Parry J, Norppa H, Eastmond DA, Tucker JD, Thomas P. 2011. Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells. Mutagenesis 26: 125-132.
16. Gisselsson D. 2005. Mitotic instability in cancer: Is there method in the madness? Cell Cycle 4: 1007-1010.
17. Gisselsson D, Hoglund M. 2005. Connecting mitotic instability and chromosome aberrations in cancer-Can telomeres bridge the gap? Semin Cancer Biol 15: 13-23.
18. Gonzalo S, Jaco I, Fraga MF, Chen T, Li E, Esteller M, Blasco MA. 2006. DNA methyltransferases control telomere length and telomere recombination in mammalian cells. Nat Cell Biol 8: 416-424.
19. Hirano T. 2006. At the heart of the chromosome: SMC proteins in action. Nat Rev Mol Cell Biol 7: 311-322.
20. Huang Y, Fenech M, Shi Q. 2011. Micronucleus formation detected by live-cell imaging. Mutagenesis 26: 133-138.
21. Jacky PB, Beek B, Sutherland GR. 1983. Fragile sites in chromosomes: Possible model for the study of spontaneous chromosome breakage. Science 220: 69-70.
22. Jaco I, Canela A, Vera E, Blasco MA. 2008. Centromere mitotic recombination in mammalian cells. J Cell Biol 181: 885-892.
23. Kimura M, Umegaki K, Higuchi M, Thomas P, Fenech M. 2004. Methylenetetrahydrofolate reductase C677T polymorphism, folic acid and riboflavin are important determinants of genome stability in cultured human lymphocytes. J Nutr 134: 48-56.
24. Lindberg HK, Wang X, Jarventaus H, Falck GC, Norppa H, Fenech M. 2007. Origin of nuclear buds and micronuclei in normal and folate-deprived human lymphocytes. Mutat Res 617: 33-45.
25. Naim V, Rosselli F. 2009. The FANC pathway and BLM collaborate during mitosis to prevent micro-nucleation and chromosome abnormalities. Nat Cell Biol 11: 761-768.
26. Nasmyth K, Haering CH. 2005. The structure and function of SMC and kleisin complexes. Annu Rev Biochem 74: 595-648.
27. Newman JP, Banerjee B, Fang W, Poonepalli A, Balakrishnan L, Low GK, Bhattacharjee RN, Akira S, Jayapal M, Melendez AJ, Baskar R, Lee HW, Hande MP. 2008. Short dysfunctional telomeres impair the repair of arsenite-induced oxidative damage in mouse cells. J Cell Physiol 214: 796-809.
28. Thomas P, Umegaki K, Fenech M. 2003. Nucleoplasmic bridges are a sensitive measure of chromosome rearrangement in the cytokinesis-block micronucleus assay. Mutagenesis 18: 187-194.
29. Tusell L, Soler D, Agostini M, Pampalona J, Genesca A. 2008. The number of dysfunctional telomeres in a cell: One amplifies; more than one translocate. Cytogenet Genome Res 122: 315-325.
30. van Steensel B, Smogorzewska A, de Lange T. 1998. TRF2 protects human telomeres from end-to-end fusions. Cell 92: 401-413.
31. Wang X, Fenech M. 2003. A comparison of folic acid and 5-methyltetrahydrofolate for prevention of DNA damage and cell death in human lymphocytes in vitro. Mutagenesis 18: 81-86.