Blocking human contaminant DNA during PCR allows amplification of rare mammal species from sedimentary ancient DNA

Molecular Ecology

Volumn 21, Issue 8, 2012, Pages 1806-1815

  Boessenkool, Sanne ^a   Epp, Laura S ^a   Haile, James ^b,c   Bellemain, Eva ^a   Edwards, Mary ^d   Coissac, Eric ^e   Willerslev, Eske ^b   Brochmann, Christian ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c MURDOCH UNIVERSITY   (Australia)

^d UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^e UNIVERSITÉ JOSEPH FOURIER   (France)

Author keywords

blocking primer; contamination; environmental DNA; metabarcoding; PCR bias; pyrosequencing

Indexed keywords

DNA; ICE; PRIMER DNA;

ANIMAL; ARTICLE; CHEMISTRY; CLASSIFICATION; DNA CONTAMINATION; DNA SEQUENCE; EVALUATION; FOSSIL; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; PERISSODACTYLA; POLYMERASE CHAIN REACTION; RUSSIAN FEDERATION; SEDIMENT;

ANIMALS; DNA; DNA CONTAMINATION; DNA PRIMERS; FOSSILS; GEOLOGIC SEDIMENTS; HUMANS; ICE; PERISSODACTYLA; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SIBERIA;

COELODONTA ANTIQUITATIS; MAMMALIA;

EID: 84859583189     PISSN: 09621083     EISSN: 1365294X     Source Type: Journal    
DOI: 10.1111/j.1365-294X.2011.05306.x     Document Type: Article

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