Designing isothermal titration calorimetry experiments for the study of 1:1 binding: Problems with the "standard protocol"

Analytical Biochemistry

Volumn 424, Issue 2, 2012, Pages 211-220

  Tellinghuisen, Joel ^a  

^a VANDERBILT UNIVERSITY   (United States)

Author keywords

Data analysis; Experiment design; ITC; Nonlinear least squares; Statistical errors

Indexed keywords

CALORIMETERS; CALORIMETRY; DATA REDUCTION; ISOTHERMS; TITRATION;

ESTIMATES OF PARAMETERS; EXPERIMENT DESIGN; FEASIBILITY ASSESSMENT; ISOTHERMAL TITRATION CALORIMETRY; NON-LINEAR LEAST SQUARES; STANDARD DEVIATION; STANDARD PROTOCOLS; STATISTICAL ERRORS;

PARAMETER ESTIMATION;

ANALYTICAL ERROR; ARTICLE; DATA ANALYSIS; EXPERIMENTAL DESIGN; HEAT; ISOTHERMAL TITRATION CALORIMETRY; PRIORITY JOURNAL; SCIENTIFIC LITERATURE; STANDARDIZATION;

EID: 84859573207     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.12.035     Document Type: Article

References (31)

1. T. Wiseman, S. Williston, J.F. Brandts, and L.-N. Lin Rapid measurement of binding constants and heats of binding using a new titration calorimeter Anal. Biochem. 179 1989 131 137 (Pubitemid 19149635)
2. J. Tellinghuisen Volume errors in isothermal titration calorimetry Anal. Biochem. 333 2004 405 406 (Pubitemid 39278067)
3. D.R. Bundle, and B.W. Sigursjold Determination of accurate thermodynamics of binding by titration microcalorimetry Methods Enzymol. 247 1994 288 305
4. A. Velazquez-Campoy, and E. Freire ITC in the post-genomic era ? Priceless Biophys. Chem. 115 2005 115 124
5. E.A. Lewis, and K.P. Murphy Isothermal titration calorimetry Methods Mol. Biol. 305 2005 1 16
6. M.W. Freyer, and E.A. Lewis Isothermal titration calorimetry: experiment design, data analysis, and probing macromolecule/ligand binding and kinetic interactions Methods Cell Biol. 84 2008 79 113
7. T. Biswas, and O.V. Tsodikov An easy-to-use tool for planning and modeling a calorimetric titration Anal. Biochem. 406 2010 91 93
8. L.D. Hansen, G.W. Fellingham, and D.J. Russell Simultaneous determination of equilibrium constants and enthalpy changes by titration calorimetry: Methods, instruments, and uncertainties Anal. Biochem. 409 2011 220 229
9. N.A. Demarse, C.F. Quinn, D.L. Eggett, D.J. Russell, and L.D. Hansen Calibration of nanowatt isothermal titration calorimeters with overflow reaction vessels Anal. Biochem. 417 2011 247 255
10. J. Broecker, C. Vargas, and S. Keller Revisiting the optimal c value for isothermal titration calorimetry Anal. Biochem. 418 2011 307 309
11. W.B. Turnbull, and A.H. Daranas On the value of c: can low affinity systems be studied by isothermal titration calorimetry? J. Am. Chem. Soc. 125 2003 14859 14866 (Pubitemid 37475576)
12. J. Tellinghuisen Optimizing experimental parameters in isothermal titration calorimetry J. Phys. Chem. B 109 2005 20027 20035 (Pubitemid 41598517)
13. J. Tellinghuisen Optimizing experimental parameters in isothermal titration calorimetry: variable volume procedures J. Phys. Chem. B 111 2007 11531 11537 (Pubitemid 47598336)
14. J. Tellinghuisen Isothermal titration calorimetry at very low c Anal. Biochem. 373 2008 395 397
15. D.G. Myszka, Y.N. Abdiche, F. Arisaka, O. Byron, E. Eisenstein, P. Hensley, J.A. Thomson, C.R. Lombardo, F. Schwarz, W. Stafford, and M.L. Doyle The ABRF-MIRG '02 study: assembly state, thermodynamic, and kinetic analysis of an enzyme/inhibitor interaction J. Biomol. Technol. 14 2003 247 269
16. J. Tellinghuisen, and J.D. Chodera Systematic errors in isothermal titration calorimetry: concentrations and baselines Anal. Biochem. 414 2011 297 299
17. J. Tellinghuisen A study of statistical error in isothermal titration calorimetry Anal. Biochem. 321 2003 79 88 (Pubitemid 37083269)
18. J. Tellinghuisen Statistical error in isothermal titration calorimetry Methods Enzymol. 383 2004 245 282 (Pubitemid 38401794)
19. J. Tellinghuisen A Monte Carlo study of precision, bias, inconsistency, and non-Gaussian distributions in nonlinear least squares J. Phys. Chem. A 104 2000 2834 2844
20. J. Tellinghuisen Stupid statistics! Methods Cell Biol. 84 2008 739 780
21. J. Tellinghuisen Understanding least squares through Monte Carlo calculations J. Chem. Educ. 82 2005 157 166 (Pubitemid 40085203)
22. J. Tellinghuisen Calibration in isothermal titration calorimetry: heat and cell volume from heat of dilution of NaCl(aq) Anal. Biochem. 360 2007 47 55 (Pubitemid 44894350)
23. J. Tellinghuisen Statistical error in isothermal titration calorimetry: variance function estimation from generalized least squares Anal. Biochem. 343 2005 106 115 (Pubitemid 40978615)
24. Q.C. Zeng, E. Zhang, H. Dong, and J. Tellinghuisen Weighted least squares in calibration: estimating data variance functions in high-performance liquid chromatography J. Chromatogr. A 1206 2008 147 152
25. 2+-binding constants by global least-squares analysis of isothermal titration calorimetry data Anal. Biochem. 319 2003 216 233 (Pubitemid 36842730)
26. J.C.D. Houtman, P.H. Brown, B. Bowden, H. Yamaguchi, E. Appella, L.E. Samelson, and P. Schuck Studying multisite binary and ternary protein interactions by global analysis of isothermal titration calorimetry data in SEDPHAT: application to adaptor protein complexes in cell signaling Protein Sci. 16 2007 30 42 (Pubitemid 46036496)
27. L. Indyk, and H.F. Fisher Theoretical aspects of isothermal titration calorimetry Methods Enzymol. 295 1998 350 364 (Pubitemid 29349922)
28. G.M.K. Poon Explicit formulation of titration models for isothermal titration calorimetry Anal. Biochem. 400 2010 229 236
29. N. Markova, and D. Hallén The development of a continuous isothermal titration calorimetric method for equilibrium studies Anal. Biochem. 331 2004 77 88 (Pubitemid 38881560)
30. M. El Harrous, O.L. Mayorga, and A. Parody-Morreale Description of a new Gill titration calorimeter for the study of biochemical reactions: II. Operational characterization of the instrument Meas. Sci. Technol. 5 1994 1071 1077
31. L.S. Mizoue, and J. Tellinghuisen The role of backlash in the "first injection anomaly" in isothermal titration calorimetry Anal. Biochem. 326 2004 125 127 (Pubitemid 38177493)