Random amplification and pyrosequencing for identification of novel viral genome sequences

Journal of Biomolecular Techniques

Volumn 23, Issue 1, 2012, Pages 4-10

  Hang, Jun ^a   Forshey, Brett M ^b   Kochel, Tadeusz J ^b,c   Li, Tao ^a   Solórzano, Víctor Fiestas ^d   Halsey, Eric S ^b   Kuschner, Robert A ^a  

^a WALTER REED ARMY INSTITUTE OF RESEARCH   (United States)

^b US Naval Medical Research Unit No 6   (Peru)

^c NAVAL MEDICAL RESEARCH CENTER   (United States)

^d INSTITUTO NACIONAL DE SALUD   (Peru)

Author keywords

Microbial; Next generation dna sequencing; Orthobunyavirus; Pathogen discovery

Indexed keywords

VIRUS RNA;

ACCURACY; ADOLESCENT; ADULT; ANIMAL CELL; ARTICLE; FEMALE; GENE AMPLIFICATION; GENE SEQUENCE; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; ORTHOBUNYAVIRUS; POLYMERASE CHAIN REACTION; PYROSEQUENCING; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; VIRUS CULTURE; VIRUS GENOME;

ADOLESCENT; AEDES; ANIMALS; CERCOPITHECUS AETHIOPS; CONTIG MAPPING; DATA MINING; DNA PRIMERS; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENOME, VIRAL; HUMANS; MIDDLE AGED; MOLECULAR SEQUENCE DATA; MOLECULAR TYPING; ORTHOBUNYAVIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SEQUENCE ANALYSIS, DNA; VERO CELLS; VIRUS CULTIVATION;

CARAPARU VIRUS; OROPOUCHE VIRUS; ORTHOBUNYAVIRUS;

EID: 84859474927     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: 10.7171/jbt.12-2301-001     Document Type: Article

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