PEGylation, detection and chromatographic purification of site-specific PEGylated CD133-Biotin antibody in route to stem cell separation

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 893-894, Issue , 2012, Pages 182-186

  González González, Mirna ^a   Mayolo Deloisa, Karla ^a   Rito Palomares, Marco ^a  

^a SCHOOL OF ENGINEERING AND SCIENCES   (Mexico)

Author keywords

Antibody PEGylation; CD133; Stem progenitor cells separation; Streptavidin biotin conjugation

Indexed keywords

AFFINITY COLUMN; AQUEOUS TWO PHASE SYSTEM; CD133; CELL SEPARATION; CHROMATOGRAPHIC PURIFICATION; HIGH SELECTIVITY; IMMUNOCHEMICAL TECHNIQUES; ION-EXCHANGE CHROMATOGRAPHY; NEURAL GROWTH; NEURAL STEM CELL; NEURONAL FUNCTION; PEGYLATION; PROCESS STEPS; RECOVERY AND PURIFICATIONS; SILVER STAINING; SITE-SPECIFIC; STREPTAVIDIN-BIOTIN CONJUGATION;

ANTIBODIES; CHEMICAL DETECTION; CHROMATOGRAPHIC ANALYSIS; COENZYMES; ELECTROPHORESIS; FLUIDIZATION; MOLECULAR BIOLOGY; PURIFICATION; SEPARATION; STEM CELLS;

CHROMATOGRAPHY;

BARIUM CHLORIDE; CD133 BIOTIN ANTIBODY; PROTEIN ANTIBODY; STREPTAVIDIN; UNCLASSIFIED DRUG;

ARTICLE; CELL SEPARATION; CHEMICAL MODIFICATION; CONJUGATION; ELUTION; ION EXCHANGE CHROMATOGRAPHY; NEURAL STEM CELL; PEGYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; SILVER STAINING;

ANTIBODIES; ANTIGENS, CD; BARIUM COMPOUNDS; BIOTIN; CELL SEPARATION; CHLORIDES; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOPROTEINS; PEPTIDES; POLYETHYLENE GLYCOLS; STREPTAVIDIN;

EID: 84859440410     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2012.03.002     Document Type: Article

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