Detection of influenza A viruses with a portable real-time PCR instrument

Journal of Virological Methods

Volumn 181, Issue 2, 2012, Pages 188-191

  Mölsä, Markos ^a   Koskela, Katja A ^a   Rönkkö, Esa ^b   Ikonen, Niina ^b   Ziegler, Thedi ^b   Nikkari, Simo ^a  

^a Centres for Military Medicine and for Biological Threat Preparedness   (Finland)

^b NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

Author keywords

Influenza A; Influenza A(H1N1)pdm09; Portable detector; Rapid real time RT PCR; RAZOR EX

Indexed keywords

INFLUENZA VIRUS HEMAGGLUTININ; MATRIX PROTEIN;

ARTICLE; ASSAY; INFLUENZA VIRUS A; INFLUENZA VIRUS A H1N1; INFLUENZA VIRUS B; INFLUENZA VIRUS C; INSTRUMENT; PATIENT IDENTIFICATION; PORTABLE EQUIPMENT; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY VIRUS; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUS GENE; VIRUS IDENTIFICATION; VIRUS STRAIN;

HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; HUMANS; INFLUENZA A VIRUS; INFLUENZA, HUMAN; POINT-OF-CARE SYSTEMS; REAL-TIME POLYMERASE CHAIN REACTION; TIME FACTORS; VIRAL MATRIX PROTEINS; VIROLOGY;

INFLUENZA A VIRUS;

EID: 84859422302     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.02.001     Document Type: Article

References (10)

1. Aho M., Lyytikäinen O., Nyholm J., Kuitunen T., Rönkkö E., Santanen R., Ziegler T., Nikkari S. Outbreak of 2009 pandemic influenza A(H1N1) in a Finnish garrison - a serological survey. Euro Surveill. 2010, 15(45).
2. Cheng P.K., Wong K.K., Mak G.C., Wong A.H., Ng A.Y., Chow S.Y., Lam R.K., Lau C.S., Ng K.C., Lim W. Performance of laboratory diagnostics for the detection of influenza A(H1N1)v virus as correlated with the time after symptom onset and viral load. J. Clin. Virol. 2010, 47:182-185.
3. Dawood F.S., Jain S., Finelli L., Shaw M.W., Lindstrom S., Garten R.J., Gubareva L.V., Xu X., Bridges C.B., Uyeki T.M. Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N. Engl. J. Med. 2009, 360:2605-2615.
4. Koskela K.A., Matero P., Blatny J.M., Fykse E.M., Olsen J.S., Nuotio L.O., Nikkari S. A multiplatform real-time polymerase chain reaction detection assay for Vibrio cholerae. Diagn. Microbiol. Infect. Dis. 2009, 65:339-344.
5. Matero P., Hemmilä H., Tomaso H., Piiparinen H., Rantakokko-Jalava K., Nuotio L., Nikkari S. Rapid field detection assays for Bacillus anthracis, Brucella spp., Francisella tularensis and Yersinia pestis. Clin. Microbiol. Infect. 2011, 17:34-43.
6. Munster V.J., Wallensten A., Baas C., Rimmelzwaan G.F., Schutten M., Olsen B., Osterhaus A.D., Fouchier R.A. Mallards and highly pathogenic avian influenza ancestral viruses, northern Europe. Emerg. Infect. Dis. 2005, 11:1545-1551.
7. Ortiz de la Tabla V., Antequera P., Masia M., Ros P., Martin C., Gazquez G., Bunuel F., Sanchez V., Robledano C., Gutierrez F. Clinical evaluation of rapid point-of-care testing for detection of novel influenza A (H1N1) virus in a population-based study in Spain. Clin. Microbiol. Infect. 2010, 16:1358-1361.
8. Rönkkö E., Ikonen N., Kontio M., Haanpää M., Kallio-Kokko H., Mannonen L., Lappalainen M., Julkunen I., Ziegler T. Validation and diagnostic application of NS and HA gene-specific real-time reverse transcription-PCR assays for detection of 2009 pandemic influenza A (H1N1) viruses in clinical specimens. J. Clin. Microbiol. 2011, 49:2009-2011.
9. Wang W., Ren P., Mardi S., Hou L., Tsai C., Chan K.H., Cheng P., Sheng J., Buchy P., Sun B., Toyoda T., Lim W., Peiris J.S., Zhou P., Deubel V. Design of multiplexed detection assays for identification of avian influenza a virus subtypes pathogenic to humans by SmartCycler real-time reverse transcription-PCR. J. Clin. Microbiol. 2009, 47:86-92.
10. Ward C.L., Dempsey M.H., Ring C.J., Kempson R.E., Zhang L., Gor D., Snowden B.W., Tisdale M. Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement. J. Clin. Virol. 2004, 29:179-188.