High-level expression of Falcipain-2 in Escherichia coli by codon optimization and auto-induction

Protein Expression and Purification

Volumn 83, Issue 1, 2012, Pages 59-69

  Sarduy, Emir Salas ^a,c   Muñoz, Aymara Cabrera ^a   Trejo, Sebastián Alejandro ^b,c   Chavéz Planes, María De Los A ^a,c  

^a UNIVERSITY OF HAVANA   (Cuba)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^c Iberoamerican Program of Science and Technology for Development   (Cuba)

Author keywords

Auto induction; Codon optimization; Falcipain 2; Plasmodium falciparum; Synthetic gene

Indexed keywords

CYSTEINE PROTEINASE; FALCIPAIN 2; ISOPROPYL THIOGALACTOSIDE; PROTOZOAL PROTEIN; RECOMBINANT PROTEIN; SYNTHETIC DNA;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CODON; DRUG EFFECT; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HIGH THROUGHPUT SCREENING; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; PLASMODIUM FALCIPARUM; PROTEIN ENGINEERING; PROTEIN FOLDING; SEQUENCE ALIGNMENT; SOLUBILITY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; CODON; CYSTEINE ENDOPEPTIDASES; ESCHERICHIA COLI; GENES, SYNTHETIC; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROGEN-ION CONCENTRATION; ISOPROPYL THIOGALACTOSIDE; MOLECULAR SEQUENCE DATA; PLASMODIUM FALCIPARUM; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SOLUBILITY;

ESCHERICHIA COLI; PLASMODIUM FALCIPARUM;

EID: 84859347090     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.03.008     Document Type: Article

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