Gene cloning, expression and characterization of a neoagarotetraose-producing β-agarase from the marine bacterium Agarivorans sp. HZ105

World Journal of Microbiology and Biotechnology

Volumn 28, Issue 4, 2012, Pages 1691-1697

  Lin, Bokun ^a   Lu, Guoyong ^a   Zheng, Yandan ^a   Xie, Wei ^a   Li, Shengkang ^a   Hu, Zhong ^a  

^a SHANTOU UNIVERSITY   (China)

Author keywords

Agarase; Agarivorans sp. HZ105; Characterization; Cloning; Expression; Neoagarotetraose

Indexed keywords

AGARASE; AGARIVORANS SP. HZ105; AGAROSE; AMINO ACID SEQUENCE; DEGREES OF POLYMERIZATIONS; DITHIOTHREITOL; DOMINANT PRODUCT; E. COLI; EXPRESSION; GENE CLONING; GENE SEQUENCES; GLYCOSIDE HYDROLASES; MARINE BACTERIUM; NEOAGARO-OLIGOSACCHARIDES; NEOAGAROTETRAOSE; POTENTIAL APPLICATIONS; VIBRIO SP;

AMINO ACIDS; BACTERIA; CHARACTERIZATION; CLONING; ESCHERICHIA COLI; GENE EXPRESSION; SODIUM;

ENZYME ACTIVITY;

AGARASE; DITHIOTHREITOL; DODECYL SULFATE SODIUM; ENZYME ACTIVATOR; ENZYME INHIBITOR; GALACTOSIDE; GLYCOSIDASE; MERCAPTOETHANOL; NEOAGAROTETRAOSE; OLIGOSACCHARIDE; RECOMBINANT PROTEIN; SEPHAROSE;

ALTEROMONADACEAE; AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HYDROLYSIS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY;

ALTEROMONADACEAE; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DITHIOTHREITOL; ENZYME ACTIVATORS; ENZYME INHIBITORS; ESCHERICHIA COLI; GALACTOSIDES; GENE EXPRESSION; GLYCOSIDE HYDROLASES; HYDROLYSIS; KINETICS; MERCAPTOETHANOL; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OLIGOSACCHARIDES; RECOMBINANT PROTEINS; SEPHAROSE; SEQUENCE HOMOLOGY, AMINO ACID; SODIUM DODECYL SULFATE;

AGARIVORANS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; VIBRIO; VIBRIO SP.;

EID: 84859268610     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-011-0977-y     Document Type: Article

References (22)

1. Allouch J, Jam M, Helbert W, Barbeyron T, Kloareg B, Henrissat B, Czjzek M (2003) The three-dimensional structures of two β-agarases. J Biol Chem 278: 47171-47180.
2. Du Z, Wang J, Yang L, Chen G (2011) Identification of a marine agarolytic bacterium Agarivorans albus QM38 and cloning and sequencing its beta-agarase genes. Acta Oceanol Sin 30: 118-124.
3. Fu XT, Kim SM (2010) Agarase: review of major sources, categories, purification method, enzyme characteristics and applications. Mar Drugs 8: 200-218.
4. Fu XT, Lin H, Kim SM (2008) Purification and characterization of a novel beta-agarase, AgaA34, from Agarivorans albus YKW-34. Appl Microbiol Biotechnol 78: 265-273.
5. Fu XT, Pan C-H, Lin H, Kim SM (2009) Gene cloning, expression, and characterization of a β-agarase, AgaB34, from Agarivorans albus YKW-34. J Microbiol Biotechnol 19: 257-264.
6. Hu B, Gong Q, Wang Y, Ma Y, Li J, Yu W (2006) Prebiotic effects of neoagaro-oligosaccharides prepared by enzymatic hydrolysis of agarose. Anaerobe 12: 260-266.
7. Hu Z, Lin BK, Xu Y, Zhong MQ, Liu GM (2009) Production of agarase from a marine agarolytic bacterium Agarivorans sp. HZ105. J Appl Microbiol 106: 181-190.
8. Jam M, Flament D, Allouch J, Potin P, Thion L, Kloareg B, Czjzek M, Helbert W, Michel G, Barbeyron T (2005) The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours. Biochem J 385: 703-713.
9. Jang MK, Lee DG, Kim NY, Yu KH, Jang HJ, Lee SW, Jang HJ, Lee Y, Lee S (2009) Purification and characterization of neoagarotetraose from hydrolyzed agar. J Microbiol Biotechnol 19: 1197-1200.
10. Kim HT, Lee S, Lee D, Kim HS, Bang WG, Kim KH, Choi IG (2010) Overexpression and molecular characterization of Aga50D from Saccharophagus degradans 2-40: an exo-type β-agarase producing neo-agarobiose. Appl Microbiol Biotechnol 86: 227-234.
11. Kobayashi R, Takisada M, Suzuki T, Kirimura K, Usami S (1997) Neoagarobiose as a novel moisturizer with whitening effect. Biosci Biotechnol Biochem 61: 162-163.
12. Lee DG, Park GT, Kim NY, Lee EJ, Jang MK, Shin YG, Park GS, Kim TM, Lee JH, Lee JH, Kim SJ, Lee SH (2006) Cloning, expression, and characterization of a glycoside hydrolase family 50 β-agarase from a marine Agarivorans isolate. Biotechnol Lett 28: 1925-1932.
13. Lee DG, Jang MK, Lee OH, Kim NY, Ju SA, Lee SH (2008) Over-production of a glycoside hydrolase family 50 β-agarase from Agarivorans sp. JA-1 in Bacillus subtilis and the whitening effect of its product. Biotechnol Lett 30: 911-918.
14. Long M, Yu Z, Xu X (2010) A Novel β-Agarase with high pH stability from marine Agarivorans sp. LQ48. Mar Biotechnol 12: 62-69.
15. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-428.
16. Nikapitiya C, Oh C, Lee Y, Lee S, Whang I, Lee J (2010) Characterization of a glycoside hydrolase family 50 thermostable beta agarase AgrA from marine bacteria Agarivorans sp. AG17. Fish Aquat Sci 13: 36-48.
17. Ohta Y, Hatada Y, Nogi Y, Li Z, Ito S, Horikoshi K (2004) Cloning, expression, and characterization of a glycoside hydrolase family 86 -agarase from a deep-sea Microbulbifer-like isolate. Appl Microbiol Biotechnol 66: 266-275.
18. Ohta Y, Hatada Y, Ito S, Horikoshi K (2005) High-level expression of a neoagarobiose-producing β-agarase gene from Agarivorans sp. JAMB-A11 in Bacillus subtilis and enzymic properties of the recombinant enzyme. Biotechnol Appl Biochem 41: 183-191.
19. Potin P, Richard C, Rochas C, Kloareg B (1993) Purification and characterization of the α-agarase from Atteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B. Eur J Biochem 214: 599-607.
20. Sugano Y, Matsumoto T, Kodama H, Noma M (1993) Cloning and sequencing of agaA, a unique agarase 0107 gene from a marine bacterium, Vibrio sp. strain JT0107. Appl Environ Microbiol 59: 3750-3756.
21. Sugano Y, Matsumoto T, Noma M (1994) Sequence analysis of the agaB gene encoding a new beta-agarase from Vibrio sp. strain JT0107. Biochim Biophys Acta 1218: 105-108.
22. Wang J, Jiang X, Mou H, Guan H (2004) Anti-oxidation of agar oligosaccharides produced by agarase from a marine bacterium. J Appl Phycol 16: 333-340.