Caspase pathway of elaidic acid (9t-C18:1)-induced apoptosis in human umbilical vein endothelial cells

Cell Biology International

Volumn 36, Issue 3, 2012, Pages 255-260

  Qiu, Bin ^a   Hu, Jiang Ning ^a   Liu, Rong ^a   Fan, Ya Wei ^a   Li, Jing ^a   Li, Yu ^a   Deng, Ze Yuan ^a  

^a NANCHANG UNIVERSITY   (China)

Author keywords

Apoptosis; Caspase; Elaidic acid; Human umbilical vein endothelial cell; Trans fatty acid (TFA)

Indexed keywords

BENZYLOXYCARBONYLISOLEUCYLGLUTAMYLTHREONYLASPARTYL FLUOROMETHYL KETONE; BENZYLOXYCARBONYLLEUCYLGLUTAMYLHISTIDYLASPARTYL FLUOROMETHYL KETONE; CASPASE 3; CASPASE 8; CASPASE 9; ELAIDIC ACID; MESSENGER RNA; PROTEIN BID; SECOND MITOCHONDRIAL ACTIVATOR OF CASPASE;

APOPTOSIS; ARTICLE; COLORIMETRY; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; UMBILICAL VEIN ENDOTHELIAL CELL;

APOPTOSIS; CASPASE 3; CASPASE 8; CASPASE 9; CASPASES; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; OLEIC ACID; REAL-TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION;

EID: 84859231950     PISSN: 10656995     EISSN: 10958355     Source Type: Journal    
DOI: 10.1042/CBI20110164     Document Type: Article

References (11)

1. Artwohl M, Lindenmair A, Sexl V, Maier C, Rainer G, Freudenthaler A et al. Different mechanisms of saturated versus polyunsaturated FFA-induced apoptosis in human endothelial cells. J Lipid Res 2008;49:2627-40.
2. Bråkenhielm E, Veitonmäki N, Cao R, Kihara S, Matsuzawa Y, Zhivotovsky B et al. Adiponectin-induced antiangiogenesis and antitumor activity involve caspase-mediated endothelial cell apoptosis. Proc Natl Acad Sci USA 2004;101:2476-81.
3. Harvey KA, Walker CL, Pavlin TM, Xu Z, Zaloga GP, Siddiqui RA. Long-chain saturated fatty acids induce pro-inflammatory responses and impact endothelial cell growth. Clin Nutr 2009;29:1-9.
4. Hanshaw RG, Smith BD. New reagents for phosphatidylserine recognition and detection of apoptosis. Bioorg Med Chem 2005;13:5035-42.
5. Kondoh Y, Kawada T, Urade R. Activation of caspase 3 in HepG2 cells by elaidic acid (t18:1). Biochim Biophys Acta 2007;1771:500-5.
6. Leal EC, Aveleira CA, Castilho AF, Serra AM, Baptista FI, Hosoya K et al. High glucose and oxidative/nitrosative stress conditions induce apoptosis in retinal endothelial cells by a caspaseindependent pathway. Exp Eye Res 2009;88:983-91.
7. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C (T)) method. Methods 2001;25:402-8.
8. Mallat Z, Tedgui A. Apoptosis in the vasculature: mechanisms and functional importance. Br J Pharmacol 2000;130:947-62.
9. Piro S, Spampinato D, Spadaro L, Oliveri CE, Purrello F, Rabuazzo AM. Direct apoptotic effects of free fatty acids on human endothelial cells. Nutr Metab Cardiovasc 2008;18:96-104.
10. Willett WC, Mozaffarian D. Trans fats in cardiac and diabetes risk: an overview. Curr Cardiovasc Risk Rep 2007;1:16-23.
11. Yin XM. Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins connects the death receptor and mitochondria apoptosis pathways. Cell Res 2000;10:161-7.