Transformation of tobacco and Arabidopsis plants with Stellaria media genes encoding novel hevein-like peptides increases their resistance to fungal pathogens

Transgenic Research

Volumn 21, Issue 2, 2012, Pages 313-325

  Shukurov, Rahim R ^a   Voblikova, Vera D ^a   Nikonorova, Alexandra K ^a   Komakhin, Roman A ^a   Komakhina, Vera V ^a   Egorov, Tsezi A ^b   Grishin, Eugene V ^b   Babakov, Alexey V ^a  

^a ALL RUSSIA RESEARCH INSTITUTE OF AGRICULTURAL BIOTECHNOLOGY   (Russian Federation)

^b SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

Author keywords

Antifungal resistance; Antimicrobial peptide; Arabidopsis thaliana; Hevein like peptide; Stellaria media

Indexed keywords

ANTIMICROBIAL CATIONIC PEPTIDE; HEVEIN; PLANT LECTIN; VEGETABLE PROTEIN;

ARABIDOPSIS; ARTICLE; ASCOMYCETES; DISEASE RESISTANCE; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSCRIPTION; GENETIC TRANSFORMATION; GENETICS; IMMUNOLOGY; METABOLISM; MICROBIOLOGY; PATHOGENICITY; PLANT DISEASE; PLANT GENE; RHIZOBIUM; STELLARIA; TOBACCO; TRANSGENE; TRANSGENIC PLANT;

AGROBACTERIUM; ANTIMICROBIAL CATIONIC PEPTIDES; ARABIDOPSIS; ASCOMYCOTA; DISEASE RESISTANCE; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; GENETIC VECTORS; PLANT DISEASES; PLANT LECTINS; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; STELLARIA; TOBACCO; TRANSCRIPTION, GENETIC; TRANSFORMATION, GENETIC; TRANSGENES;

ARABIDOPSIS; ARABIDOPSIS THALIANA; BIPOLARIS SOROKINIANA; FUNGI; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; STELLARIA; STELLARIA MEDIA; THIELAVIOPSIS BASICOLA;

EID: 84859104690     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-011-9534-6     Document Type: Article

References (59)

1. Aerts AM, Francois IE, Cammue BP, Thevissen K (2008) The mode of antifungal action of plant, insect and human defensins. Cell Mol Life Sci 65: 2069-2079.
2. Beintema JJ (1994) Structural features of plant chitinases and chitin-binding proteins. FEBS Lett 350: 159-163.
3. Benko-Iseppon AM, Galdino SL, Calsa T Jr, Kido EA, Tossi A, Belarmino LC, Crovella S (2010) Overview on plant antimicrobial peptides. Curr Protein Pept Sci 11: 181-188.
4. Broekaert I, Lee HI, Kush A, Chua NH, Raikhel N (1990) Wound-induced accumulation of mRNA containing a hevein sequence in laticifers of rubber tree (Hevea brasiliensis). Proc Natl Acad Sci USA 87: 7633-7637.
5. Broekaert WF, Marien W, Terras FR, De Bolle MF, Proost P, van Damme J, Dillen L, Claeys M, Rees SB, Vanderleyden J et al (1992) Antimicrobial peptides from Amaranthus caudatus seeds with sequence homology to the cysteine/glycine-rich domain of chitin-binding proteins. Biochemistry 31: 4308-4314.
6. Broekaert WF, Terras FR, Cammue BP, Osborn RW (1995) Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiol 108: 1353-1358.
7. Broekaert W, Cammue B, De Bolle MF, Thevissen K, De Samblanx GW, Osborn R (1997) Antimicrobial peptides from plants. Crit Rev Plant Sci 16: 297-323.
8. Cammue BP, De Bolle MF, Terras FR, Proost P, van Damme J, Rees SB, Vanderleyden J, Broekaert WF (1992) Isolation and characterization of a novel class of plant antimicrobial peptides form Mirabilis jalapa L. seeds. J Biol Chem 267: 2228-2233.
9. Cammue BP, Thevissen K, Hendriks M, Eggermont K, Goderis IJ, Proost P, van Damme J, Osborn RW, Guerbette F, Kader JC et al (1995) A potent antimicrobial protein from onion seeds showing sequence homology to plant lipid transfer proteins. Plant Physiol 109: 445-455.
10. Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16: 735-743.
11. Craik DJ, Daly NL, Bond T, Waine C (1999) Plant cyclotides: a unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif. J Mol Biol 294: 1327-1336.
12. De Bolle MF, Osborn RW, Goderis IJ, Noe L, Acland D, Hart CA, Torrekens S, van Leuven F, Broekaert WF (1996) Antimicrobial peptides from Mirabilis jalapa and Amaranthus caudatus: expression, processing, localization and biological activity in transgenic tobacco. Plant Mol Biol 31: 993-1008.
13. De Lucca AJ, Cleveland TE, Wedge DE (2005) Plant-derived antifungal proteins and peptides. Can J Microbiol 51: 1001-1014.
14. Epple P, Apel K, Bohlmann H (1997) Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum. Plant Cell 9: 509-520.
15. Escalona VH, Aguayo E, Martínez-Hernández GB, Artés F (2010) UV-C doses to reduce pathogen and spoilage bacterial growth in vitro and in baby spinach. Postharvest Biol Technol 56: 223-231.
16. Fernandez de Caleya R, Gonzalez-Pascual B, Garcia-Olmedo F, Carbonero P (1972) Susceptibility of phytopathogenic bacteria to wheat purothionins in vitro. Appl Microbiol 23: 998-1000.
17. Francois IEJA, De Bolle MFC, Dwyer G, Goderis IJWM, Woutors PFJ, Verhaert PD, Proost P, Schaaper WMM, Cammue BPA, Broekaert WF (2002) Transgenic expression in Arabidopsis of a polyprotein construct leading to production of two different antimicrobial proteins. Plant Physiol 128: 1346-1358.
18. Gao AG, Hakimi SM, Mittanck CA, Wu Y, Woerner BM, Stark DM, Shah DM, Liang J, Rommens CM (2000) Fungal pathogen protection in potato by expression of a plant defensin peptide. Nat Biotechnol 18: 1307-1310.
19. Garcia-Olmedo F, Molina A, Alamillo JM, Rodriguez-Palenzuela P (1998) Plant defense peptides. Biopolymers 47: 479-491.
20. Guo G, Wang HX, Ng TB (2009) Pomegranin, an antifungal peptide from pomegranate peels. Protein Pept Lett 16: 82-85.
21. Hammami R, Ben Hamida J, Vergoten G, Fliss I (2009) PhytAMP: a database dedicated to antimicrobial plant peptides. Nucleic Acids Res 37(Database issue): D963-968.
22. Horsch RB, Rogers SG, Fraley RT (1985) Transgenic plants. Cold Spring Harb Symp Quant Biol 50: 433-437.
23. Huang X, Xie W, Gong Z (2000) Characteristics and antifungal activity of a chitin binding protein from Ginkgo biloba. FEBS Lett 478: 123-126.
24. Huang RH, Xiang Y, Liu XZ, Zhang Y, Hu Z, Wang DC (2002) Two novel antifungal peptides distinct with a five-disulfide motif from the bark of Eucommia ulmoides Oliv. FEBS Lett 521: 87-90.
25. Isken O, Maquat LE (2007) Quality control of eukaryotic mRNA: safeguarding cells from abnormal mRNA function. Genes Dev 21: 1833-1856.
26. Jha S, Chattoo BB (2010) Expression of a plant defensin in rice confers resistance to fungal phytopathogens. Transgenic Res 19: 373-384.
27. Jha S, Tank HG, Prasad BD, Chattoo BB (2009) Expression of Dm-AMP1 in rice confers resistance to Magnaporthe oryzae and Rhizoctonia solani. Transgenic Res 18: 59-69.
28. Kang TJ, Yang MS (2004) Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants. BMC Biotechnol 4: 20.
29. Kiba A, Saitoh H, Nishihara M, Omiya K, Yamamura S (2003) C-terminal domain of a hevein-like protein from Wasabia japonica has potent antimicrobial activity. Plant Cell Physiol 44: 296-303.
30. Komakhin R, Komakhina V, Milyukova N, Goldenkova-Pavlova I, Fadina O, Zhuchenko A (2010) Transgenic tomato plants expressing recA and NLS-recA-licBM3 genes as a model for studying meiotic recombination. Russ J Genet 46: 1440-1448.
31. Koo JC, Lee SY, Chun HJ, Cheong YH, Choi JS, Kawabata S, Miyagi M, Tsunasawa S, Ha KS, Bae DW, Han CD, Lee BL, Cho MJ (1998) Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity. Biochim Biophys Acta 1382: 80-90.
32. Koo JC, Chun HJ, Park HC, Kim MC, Koo YD, Koo SC, Ok HM, Park SJ, Lee SH, Yun DJ, Lim CO, Bahk JD, Lee SY, Cho MJ (2002) Over-expression of a seed specific hevein-like antimicrobial peptide from Pharbitis nil enhances resistance to a fungal pathogen in transgenic tobacco plants. Plant Mol Biol 50: 441-452.
33. Lay FT, Anderson MA (2005) Defensins-components of the innate immune system in plants. Curr Protein Pept Sci 6: 85-101.
34. Lee OS, Lee B, Park N, Koo JC, Kim YH, Prasad DT, Karigar C, Chun HJ, Jeong BR, Kim DH, Nam J, Yun JG, Kwak SS, Cho MJ, Yun DJ (2003) Pn-AMPs, the hevein-like proteins from Pharbitis nil confers disease resistance against phytopathogenic fungi in tomato, Lycopersicum esculentum. Phytochemistry 62: 1073-1079.
35. Li SS, Claeson P (2003) Cys/Gly-rich proteins with a putative single chitin-binding domain from oat (Avena sativa) seeds. Phytochemistry 63: 249-255.
36. Lin P, Ng TB (2009) Brassiparin, an antifungal peptide from Brassica parachinensis seeds. J Appl Microbiol 106: 554-563.
37. Lipkin A, Anisimova V, Nikonorova A, Babakov A, Krause E, Bienert M, Grishin E, Egorov T (2005) An antimicrobial peptide Ar-AMP from amaranth (Amaranthus retroflexus L.) seeds. Phytochemistry 66: 2426-2431.
38. Marcus JP, Goulter KC, Green JL, Harrison SJ, Manners JM (1997) Purification, characterisation and cDNA cloning of an antimicrobial peptide from Macadamia integrifolia. Eur J Biochem 244: 743-749.
39. McManus AM, Nielsen KJ, Marcus JP, Harrison SJ, Green JL, Manners JM, Craik DJ (1999) MiAMP1, a novel protein from Macadamia integrifolia adopts a Greek key beta-barrel fold unique amongst plant antimicrobial proteins. J Mol Biol 293: 629-638.
40. Molina A, Segura A, Garcia-Olmedo F (1993) Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens. FEBS Lett 316: 119-122.
41. Muller PY, Janovjak H, Miserez AR, Dobbie Z (2002) Processing of gene expression data generated by quantitative real-time RT-PCR. Biotechniques 32: 1372-1379.
42. Murashige T (1973) Nutrition of plant cells and organs in vitro. In Vitro 9: 81-85.
43. Nielsen KK, Nielsen JE, Madrid SM, Mikkelsen JD (1997) Characterization of a new antifungal chitin-binding peptide from sugar beet leaves. Plant Physiol 113: 83-91.
44. Odintsova TI, Vassilevski AA, Slavokhotova AA, Musolyamov AK, Finkina EI, Khadeeva NV, Rogozhin EA, Korostyleva TV, Pukhalsky VA, Grishin EV, Egorov TA (2009) A novel antifungal hevein-type peptide from Triticum kiharae seeds with a unique 10-cysteine motif. FEBS J 276: 4266-4275.
45. Parijs J, Broekaert W, Goldstein I, Peumans W (1991) Hevein: an antifungal protein from rubber-tree (Hevea brasiliensis) latex. Planta 183: 258-264.
46. Patel SU, Osborn R, Rees S, Thornton JM (1998) Structural studies of Impatiens balsamina antimicrobial protein (Ib-AMP1). Biochemistry 37: 983-990.
47. Pieterse CM, van Loon LC (1999) Salicylic acid-independent plant defence pathways. Trends Plant Sci 4: 52-58.
48. Portieles R, Ayra C, Gonzalez E, Gallo A, Rodriguez R, Chacon O, Lopez Y, Rodriguez M, Castillo J, Pujol M, Enriquez G, Borroto C, Trujillo L, Thomma BP, Borras-Hidalgo O (2010) NmDef02, a novel antimicrobial gene isolated from Nicotiana megalosiphon confers high-level pathogen resistance under greenhouse and field conditions. Plant Biotechnol J 8: 678-690.
49. Raikhel NV, Lee HI, Broekaert WF (1993) Structure and function of chitin-binding proteins. Ann Rev Plant Physiol Plant Mol Biol 44: 591-615.
50. Reymond P, Farmer EE (1998) Jasmonate and salicylate as global signals for defense gene expression. Curr Opin Plant Biol 1: 404-411.
51. Shao F, Hu Z, Xiong YM, Huang QZ, Wang CG, Zhu RH, Wang DC (1999) A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning. Biochim Biophys Acta 1430: 262-268.
52. Swathi Anuradha T, Divya K, Jami SK, Kirti PB (2008) Transgenic tobacco and peanut plants expressing a mustard defensin show resistance to fungal pathogens. Plant Cell Rep 27: 1777-1786.
53. Tailor RH, Acland DP, Attenborough S, Cammue BP, Evans IJ, Osborn RW, Ray JA, Rees SB, Broekaert WF (1997) A novel family of small cysteine-rich antimicrobial peptides from seed of Impatiens balsamina is derived from a single precursor protein. J Biol Chem 272: 24480-24487.
54. Terras FR, Schoofs HM, De Bolle MF, van Leuven F, Rees SB, Vanderleyden J, Cammue BP, Broekaert WF (1992) Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J Biol Chem 267: 15301-15309.
55. Terras FR, Eggermont K, Kovaleva V, Raikhel NV, Osborn RW, Kester A, Rees SB, Torrekens S, van Leuven F, Vanderleyden J et al (1995) Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7: 573-588.
56. Thevissen K, Osborn RW, Acland DP, Broekaert WF (2000) Specific binding sites for an antifungal plant defensin from Dahlia (Dahlia merckii) on fungal cells are required for antifungal activity. Mol Plant Microbe Interact 13: 54-61.
57. van Damme EJ, Charels D, Roy S, Tierens K, Barre A, Martins JC, Rouge P, van Leuven F, Does M, Peumans WJ (1999) A gene encoding a hevein-like protein from elderberry fruits is homologous to PR-4 and class V chitinase genes. Plant Physiol 119: 1547-1556.
58. van den Bergh KP, Proost P, van Damme J, Coosemans J, van Damme EJ, Peumans WJ (2002) Five disulfide bridges stabilize a hevein-type antimicrobial peptide from the bark of spindle tree (Euonymus europaeus L.). FEBS Lett 530: 181-185.
59. Zhang X, Henriques R, Lin SS, Niu QW, Chua NH (2006) Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nat Protoc 1: 641-646.